FastQCFastQC Report
Thu 26 May 2016
SRR1926957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590774
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8800.14895713081482936No Hit
GTCCTACAGTGGACATTTCTAAATT6100.10325437476937036No Hit
CACACACACACACACACACACACAC5980.10122314116734994No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5920.10020752436633974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG451.6124832E-619.0634882
TCGCCTT508.3757E-515.2676831
CTAATAC400.00516140114.2964013
GTAGGAC2650.013.6673
TAGGACC1351.8189894E-1213.4013624
TCTAGAC500.001461368413.3433073
AGTGCAA500.001513721513.28114610
CTAAATT1950.013.12963919
TAAGACT801.9422314E-613.0927784
GTCCTAC3850.012.8883051
TCCTACA4150.012.8621122
GGTCTAC751.4090119E-512.7230681
TAAGCCT604.0062916E-412.6960274
GACGTGG1207.166818E-1012.6884767
ACGTGGA1457.2759576E-1212.4601798
TTAGAAC550.002990825612.130283
ATTTCTA2200.012.06865715
AGGACGT2300.011.9988845
GGACGTG2250.011.8425776
CGTGGAA1459.822543E-1111.7763849
Produced by FastQC (version 0.11.5)