##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926957_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590774 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.970261047371753 30.0 26.0 31.0 7.0 31.0 2 26.616577574503957 31.0 27.0 31.0 10.0 33.0 3 26.234724276965473 31.0 26.0 31.0 7.0 34.0 4 29.914984410282106 35.0 30.0 37.0 10.0 37.0 5 29.420527985320952 35.0 28.0 35.0 7.0 37.0 6 29.26365073615291 35.0 28.0 37.0 2.0 37.0 7 29.307268092366964 35.0 29.0 37.0 2.0 37.0 8 29.007784702779745 35.0 28.0 36.0 2.0 37.0 9 31.181910510618273 37.0 30.0 39.0 2.0 39.0 10 31.421230453608317 37.0 31.0 39.0 2.0 39.0 11 31.615572113870957 37.0 32.0 39.0 2.0 39.0 12 31.492914380118286 37.0 32.0 39.0 2.0 39.0 13 31.593973329902806 37.0 32.0 39.0 2.0 39.0 14 32.460262299965805 38.0 32.0 40.0 2.0 41.0 15 32.0318006547343 38.0 31.0 40.0 2.0 41.0 16 32.07669768811762 38.0 31.0 40.0 2.0 41.0 17 32.291720691838165 38.0 32.0 40.0 2.0 41.0 18 32.324721467092324 38.0 32.0 40.0 2.0 41.0 19 31.731296570262064 38.0 31.0 40.0 2.0 41.0 20 32.02626723586346 38.0 31.0 40.0 2.0 41.0 21 31.998576443783918 38.0 31.0 40.0 2.0 41.0 22 31.98956453736962 38.0 32.0 40.0 2.0 41.0 23 31.953665868843245 38.0 32.0 40.0 2.0 41.0 24 30.671451350262537 38.0 29.0 40.0 2.0 41.0 25 31.20038458022865 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.8149237311483013 1101 2 -0.8213069772652553 1101 3 -1.6687437754482914 1101 4 -1.209585364608735 1101 5 -1.530471282160505 1101 6 -3.5532921001319266 1101 7 -1.3445883668066578 1101 8 -0.7019289761843872 1101 9 -1.024389855154606 1101 10 -9.971127003345117 1101 11 -5.015398104529723 1101 12 -2.4143216298199945 1101 13 -1.6353528943161457 1101 14 -1.3647340126771468 1101 15 -2.5817564534613915 1101 16 -1.1587191200172242 1101 17 -0.9839605065276302 1101 18 -0.7747408979808448 1101 19 -1.7409859261266476 1101 20 -0.8866383337241501 1101 21 -0.8517456747387158 1101 22 -0.7457298862085366 1101 23 -0.7115099420322721 1101 24 -0.67616320796742 1101 25 -0.666855654657379 1102 1 -1.1706240859276278 1102 2 -1.0817392093176892 1102 3 -1.2822559634240136 1102 4 -0.9911712345824277 1102 5 -1.2280948339028726 1102 6 -1.6647603260441954 1102 7 -1.106419970829613 1102 8 -0.4901454616397096 1102 9 -0.582398504891227 1102 10 -0.4414803176974402 1102 11 -0.681979420334283 1102 12 -0.7081247750629593 1102 13 -0.8705875136449315 1102 14 -1.2223076277957148 1102 15 -0.7514785536021549 1102 16 -0.8788370825520175 1102 17 -0.7325652747108684 1102 18 -1.2516471203134714 1102 19 -0.8055990765288819 1102 20 -0.8503170832703262 1102 21 -0.8572246905286249 1102 22 -0.8425305027729735 1102 23 -1.3518108356685374 1102 24 -4.305745561995153 1102 25 -3.0342006695937798 1103 1 -1.5428014793358642 1103 2 -1.206467984268464 1103 3 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1.1626329278194838 2215 5 1.6822799441852432 2215 6 1.936188040048247 2215 7 1.3063263007616186 2215 8 0.048041886925307864 2215 9 0.5525748078556205 2215 10 0.5742371154687724 2215 11 0.2994441696939951 2215 12 -0.09732034087320329 2215 13 0.46722511465917194 2215 14 0.610567135835602 2215 15 0.4317365231056236 2215 16 0.5533564766543648 2215 17 0.004969783029594055 2215 18 -0.6378009753638096 2215 19 0.7832911616613565 2215 20 -2.9393443719263637 2215 21 -1.1412013514704 2215 22 -0.6473833614606441 2215 23 -0.3962764834199284 2215 24 -0.21749662746132614 2215 25 0.18218755910123363 2216 1 0.12656928021051428 2216 2 -0.8026249392247422 2216 3 -0.0014285531816220498 2216 4 -0.3363325249050071 2216 5 -0.05032920605523472 2216 6 -2.02645159631939 2216 7 -9.217974554390757 2216 8 -9.925811581559518 2216 9 -5.402336540247525 2216 10 -3.2397854024432 2216 11 -2.6198325276789163 2216 12 -2.1580427630345262 2216 13 -2.018227389715644 2216 14 -2.2013125485174214 2216 15 -3.4119852056889073 2216 16 -2.1936174725647746 2216 17 -2.4532323058754386 2216 18 -2.116855795363591 2216 19 -1.4109571957196962 2216 20 -2.3985766057780715 2216 21 -5.282119897381186 2216 22 -3.805005443542065 2216 23 -3.835353755296751 2216 24 -4.169842518437651 2216 25 -3.498406975407523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54634.0 3 7.0 4 393.0 5 2193.0 6 2564.0 7 2082.0 8 1728.0 9 1644.0 10 1393.0 11 1487.0 12 1390.0 13 1394.0 14 1413.0 15 1462.0 16 1530.0 17 1640.0 18 2010.0 19 2287.0 20 3053.0 21 3913.0 22 4803.0 23 5792.0 24 6650.0 25 7643.0 26 8445.0 27 9356.0 28 10329.0 29 12028.0 30 14096.0 31 17168.0 32 21690.0 33 27799.0 34 37331.0 35 50940.0 36 70515.0 37 103024.0 38 94948.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18153286578518 18.719399855460438 12.825001950944767 25.274065327809613 2 13.72089081726043 20.248974611030135 42.76312667367208 23.267007898037352 3 19.775494569565158 24.411024935255117 28.963336090358098 26.85014440482163 4 10.96928059406209 16.972234643684704 39.52955404691247 32.528930715340735 5 12.07471907544202 38.076219145704904 36.14001107275341 13.709050706099665 6 30.233944068777994 37.85414134163148 19.965834481777826 11.946080107812696 7 27.285380388935188 31.24415468465425 23.57068779099274 17.899777135417814 8 24.99715719602788 37.502100280546564 21.129246597545514 16.37149592588004 9 25.854380339348054 16.169484409019034 20.651912599760948 37.32422265187197 10 13.056671763687872 30.751632824816568 35.95618255125385 20.235512860241712 11 33.80572833252635 23.718167935829673 23.95653807996912 18.519565651674856 12 21.86078403155765 26.217906935403317 32.67012604437372 19.25118298866532 13 28.00344009710825 22.83804625508523 25.965792026491457 23.19272162131506 14 19.634144276838168 23.216686136082753 29.073763692078487 28.075405895000593 15 22.765656319410585 31.498015873015873 23.780574516496017 21.955753291077524 16 20.92730323249117 30.095198752579744 28.499692716101844 20.477805298827242 17 20.78785508586056 29.73333536493489 28.211665794770997 21.267143754433548 18 20.148707947342757 29.592441356519856 29.88125711035267 20.377593585784712 19 22.47388853804595 28.21493936248923 28.40473316278416 20.906438936680658 20 21.92149333983436 28.65479552241838 29.417142392783862 20.0065687449634 21 22.196207492580726 27.900374307378982 28.20408640050382 21.699331799536477 22 21.19520684774733 29.313701589365404 28.867771841077005 20.623319721810265 23 21.34018436846605 28.56390678303268 28.44065587092514 21.655252977576122 24 21.684521673178892 28.464458153598198 29.21562121201861 20.6353989612043 25 21.444037576330093 28.520664780739846 28.86433008120997 21.170967561720087 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 195.0 2 285.5 3 376.0 4 376.0 5 376.0 6 530.0 7 684.0 8 684.0 9 684.0 10 1072.0 11 1460.0 12 1460.0 13 1460.0 14 2372.0 15 3284.0 16 3284.0 17 3284.0 18 5557.5 19 7831.0 20 7831.0 21 7831.0 22 12242.0 23 16653.0 24 16653.0 25 16653.0 26 23314.0 27 29975.0 28 29975.0 29 29975.0 30 38612.0 31 47249.0 32 47249.0 33 47249.0 34 55129.0 35 63009.0 36 63009.0 37 63009.0 38 68848.5 39 74688.0 40 74688.0 41 74688.0 42 77578.0 43 80468.0 44 80468.0 45 80468.0 46 80908.0 47 81348.0 48 81348.0 49 81348.0 50 76355.5 51 71363.0 52 71363.0 53 71363.0 54 60961.0 55 50559.0 56 50559.0 57 50559.0 58 41031.5 59 31504.0 60 31504.0 61 31504.0 62 24182.0 63 16860.0 64 16860.0 65 16860.0 66 12433.0 67 8006.0 68 8006.0 69 8006.0 70 5625.0 71 3244.0 72 3244.0 73 3244.0 74 2262.5 75 1281.0 76 1281.0 77 1281.0 78 887.5 79 494.0 80 494.0 81 494.0 82 318.0 83 142.0 84 142.0 85 142.0 86 98.5 87 55.0 88 55.0 89 55.0 90 44.0 91 33.0 92 33.0 93 33.0 94 19.5 95 6.0 96 6.0 97 6.0 98 6.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2227586183549039 2 0.12762917799361517 3 0.12966041159563557 4 0.06736924780034327 5 0.022851378022729504 6 0.020312336020204002 7 0.2754014225405993 8 0.26524525453049724 9 0.018958180285523738 10 0.03943978577256277 11 0.016249868816163204 12 0.018450371885018635 13 0.015742060415658104 14 0.01760402455084347 15 0.01455717414781287 16 0.019465988686028838 17 0.01811183295134857 18 0.01455717414781287 19 0.022512839089059437 20 0.016588407749833268 21 0.014049365747307769 22 0.016588407749833268 23 0.018450371885018635 24 0.015234252015153002 25 0.014049365747307769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 590774.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.84778050699879 #Duplication Level Percentage of deduplicated Percentage of total 1 96.41159225786737 88.55190764030866 2 2.8206949476739713 5.181491408623186 3 0.356441878007629 0.9821518612424144 4 0.12326038804336603 0.4528477226485833 5 0.07046732927546251 0.3236133896103545 6 0.03850234869432519 0.2121813163128187 7 0.03392415276925279 0.21811006962053578 8 0.02170876877856131 0.15951217838803897 9 0.015826765626827798 0.13082879658857333 >10 0.09265146240734323 1.6365171021569134 >50 0.008109999877563934 0.5170170765436353 >100 0.005898119765157741 1.0900607438803454 >500 9.215812132140359E-4 0.5437606940759768 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 880 0.14895713081482936 No Hit GTCCTACAGTGGACATTTCTAAATT 610 0.10325437476937036 No Hit CACACACACACACACACACACACAC 598 0.10122314116734994 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 592 0.10020752436633974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCTTG 45 1.6124832E-6 19.063488 2 TCGCCTT 50 8.3757E-5 15.267683 1 CTAATAC 40 0.005161401 14.296401 3 GTAGGAC 265 0.0 13.667 3 TAGGACC 135 1.8189894E-12 13.401362 4 TCTAGAC 50 0.0014613684 13.343307 3 AGTGCAA 50 0.0015137215 13.281146 10 CTAAATT 195 0.0 13.129639 19 TAAGACT 80 1.9422314E-6 13.092778 4 GTCCTAC 385 0.0 12.888305 1 TCCTACA 415 0.0 12.862112 2 GGTCTAC 75 1.4090119E-5 12.723068 1 TAAGCCT 60 4.0062916E-4 12.696027 4 GACGTGG 120 7.166818E-10 12.688476 7 ACGTGGA 145 7.2759576E-12 12.460179 8 TTAGAAC 55 0.0029908256 12.13028 3 ATTTCTA 220 0.0 12.068657 15 AGGACGT 230 0.0 11.998884 5 GGACGTG 225 0.0 11.842577 6 CGTGGAA 145 9.822543E-11 11.776384 9 >>END_MODULE