FastQCFastQC Report
Thu 26 May 2016
SRR1926957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590774
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14640.24781049944648886No Hit
GGTATCAACGCAGAGTACTTTTTTT9610.16266795762846706No Hit
CACACACACACACACACACACACAC8780.14861859188115928No Hit
TATCAACGCAGAGTACTTTTTTTTT8050.13626192080220184No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG7190.12170474665438899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA350.002191033716.26003319
TCCAACG508.8172616E-515.17603118
GGACTAG400.005132104314.308671
CGTTTCC400.005329482714.22752914
TAGGACC2250.013.5494384
CTATTAC655.2354546E-513.2080031
ATAGGAC1102.6138878E-912.9923983
GTTCTAG751.41386845E-512.7188181
CTTGTAC751.4312976E-512.7036783
GACGTGG1352.7284841E-1112.6509757
TTACACT1301.8735591E-1012.4583184
TAGCCCT1800.012.1733244
GTGTTAC550.002971665712.140691
TCTAGCC550.002998303612.1262393
GGACGTG2050.012.082966
CTAGAAC951.0002386E-612.0350633
CTAGGAC951.0002386E-612.0350633
CTGTAGG4300.011.9793511
GTCCTAC3100.011.6931071
GTAGGAC4100.011.6192183
Produced by FastQC (version 0.11.5)