##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926957_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590774 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.76352039866345 31.0 30.0 33.0 25.0 34.0 2 30.09627708734643 31.0 30.0 34.0 26.0 34.0 3 30.153022983408206 31.0 30.0 34.0 26.0 34.0 4 33.7260746072102 35.0 35.0 37.0 28.0 37.0 5 33.664340339960795 37.0 35.0 37.0 28.0 37.0 6 33.63073696540471 37.0 35.0 37.0 29.0 37.0 7 33.6212764949033 37.0 35.0 37.0 28.0 37.0 8 33.636050333968655 37.0 35.0 37.0 28.0 37.0 9 35.15266074674918 39.0 35.0 39.0 29.0 39.0 10 35.04660326960902 39.0 35.0 39.0 28.0 39.0 11 35.082982663421205 39.0 35.0 39.0 29.0 39.0 12 34.96251189118004 39.0 35.0 39.0 28.0 39.0 13 34.98205743651549 39.0 35.0 39.0 28.0 39.0 14 36.058081432155106 40.0 36.0 41.0 27.0 41.0 15 36.04746654388988 40.0 36.0 41.0 27.0 41.0 16 36.02299017898554 40.0 36.0 41.0 27.0 41.0 17 36.00430960062562 40.0 36.0 41.0 27.0 41.0 18 35.97652909572865 40.0 36.0 41.0 27.0 41.0 19 36.05998740635167 40.0 36.0 41.0 27.0 41.0 20 36.02338457684326 40.0 36.0 41.0 27.0 41.0 21 35.967314065954156 40.0 36.0 41.0 27.0 41.0 22 35.879788210043095 40.0 36.0 41.0 27.0 41.0 23 35.81601255302366 40.0 36.0 41.0 27.0 41.0 24 35.736201999410945 40.0 36.0 41.0 27.0 41.0 25 35.62866679982531 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2631687369885256 1101 2 0.22891462574987642 1101 3 0.29697173281955713 1101 4 0.25307091066362375 1101 5 0.018202673495153476 1101 6 0.1280167725201835 1101 7 0.10995623937343879 1101 8 0.09179121238760501 1101 9 0.35769850332587083 1101 10 0.3334326322521335 1101 11 0.2155687284403811 1101 12 0.299956966663423 1101 13 0.3024864166467083 1101 14 0.4023823064714165 1101 15 0.4390356462967304 1101 16 0.4620420679762418 1101 17 0.3613969792040095 1101 18 0.3696511108545977 1101 19 0.27235613563742334 1101 20 0.34793628477623173 1101 21 0.3944373023027623 1101 22 0.3765107485206727 1101 23 0.35250479142944613 1101 24 0.43911088533825904 1101 25 0.41617433643635593 1102 1 -0.5276847079378761 1102 2 -0.3454954023394734 1102 3 -0.2512024593727489 1102 4 -0.2712993082549673 1102 5 -0.5531219846236866 1102 6 -0.33017595482203177 1102 7 -0.4364077502061079 1102 8 -0.552852384068423 1102 9 -0.45921880187989217 1102 10 -0.7741706008894766 1102 11 -0.712531450541654 1102 12 -0.3886232153759508 1102 13 -0.442654635207127 1102 14 -0.49703106558827415 1102 15 -0.6569343917289885 1102 16 -0.4373152527710218 1102 17 -0.4185226381183895 1102 18 -0.5323176914944625 1102 19 -0.5223470153380632 1102 20 -0.48961045119023794 1102 21 -0.5312679569664027 1102 22 -0.6691748927891439 1102 23 -0.5002382196725321 1102 24 -0.5616598202076375 1102 25 -0.40521051314867407 1103 1 -0.7352069400267247 1103 2 -0.697153639473175 1103 3 -0.4833286321073942 1103 4 -0.5160684934859106 1103 5 -0.7218889562978816 1103 6 -0.6877606040633708 1103 7 -0.6456184627912123 1103 8 -0.6989754423801955 1103 9 -0.6757561299109867 1103 10 -0.7503143791219742 1103 11 -0.6536328237190077 1103 12 -0.7607156699787367 1103 13 -0.7789762701504372 1103 14 -0.7381800559747447 1103 15 -0.8789605683244872 1103 16 -0.8604983988529042 1103 17 -0.8328283920789872 1103 18 -0.6879693394359876 1103 19 -0.42001891989772844 1103 20 -0.645833505641157 1103 21 -0.7167485690405826 1103 22 -0.8403947942086489 1103 23 -0.9055237298844929 1103 24 -0.9066400655006106 1103 25 -0.6335581959923218 1104 1 -0.032243898582994035 1104 2 -0.04702889709330904 1104 3 0.3092345021678753 1104 4 -0.013217959830697623 1104 5 -0.03386977447672024 1104 6 -0.08349539848440202 1104 7 -0.20537372051224878 1104 8 -0.18830107675006502 1104 9 -0.3136475422368932 1104 10 -0.09200381221894105 1104 11 -0.06687948064845273 1104 12 0.17055241388245435 1104 13 -0.053439777013160494 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0.5216681596401003 1106 1 0.3619975189142366 1106 2 0.41365770312817673 1106 3 0.572800393640744 1106 4 0.5748219072789382 1106 5 0.4401058622896201 1106 6 0.6507238706185916 1106 7 0.5986129904512083 1106 8 0.5702814260184823 1106 9 0.5490324134663211 1106 10 0.7518500083790656 1106 11 0.41817035323859386 1106 12 0.6013386818780191 1106 13 0.6121194699414403 1106 14 0.5616809489771057 1106 15 0.5536180042392544 1106 16 0.6020751032925702 1106 17 0.5040668470252925 1106 18 0.7328173574009327 1106 19 0.5719112950022378 1106 20 0.5625884338821194 1106 21 0.5649724469602404 1106 22 0.6693726981037358 1106 23 0.6796592970795388 1106 24 0.5115988092196915 1106 25 0.5871651836811083 1107 1 0.34944352765472786 1107 2 0.4770935667881915 1107 3 0.5781810221651114 1107 4 0.5650965061273681 1107 5 0.5254208461756278 1107 6 0.43634900480073924 1107 7 0.5074564585549766 1107 8 0.3586308369512494 1107 9 0.35291602401846234 1107 10 0.24249637518121858 1107 11 0.24272377683936952 1107 12 0.27379595282226177 1107 13 0.27274687839737055 1107 14 0.25643580652266706 1107 15 0.20479171145106534 1107 16 0.45558640006527895 1107 17 0.25403136835434026 1107 18 0.4212952193167325 1107 19 0.30637557920033487 1107 20 0.3555761503778214 1107 21 0.3957943755686699 1107 22 0.5962405439965224 1107 23 0.5264460211428101 1107 24 0.5463036206126546 1107 25 0.4184007268807832 1108 1 0.8056449567477237 1108 2 0.6077330076685961 1108 3 0.8218439857075914 1108 4 0.8334295786773112 1108 5 0.8748184365370761 1108 6 0.971372214522745 1108 7 0.8294848317881645 1108 8 0.7643315619290831 1108 9 0.9085411218122346 1108 10 0.9164780160303252 1108 11 0.9215429496344427 1108 12 0.988029510429314 1108 13 0.8553239398900914 1108 14 0.8830591831460382 1108 15 0.889543706444023 1108 16 0.9298210008059087 1108 17 1.0039661725060114 1108 18 1.20513186459916 1108 19 1.0529593167479305 1108 20 1.2329297205024758 1108 21 1.2094907193854922 1108 22 1.3759568116645937 1108 23 1.2053564681255438 1108 24 1.0842606435094382 1108 25 1.2231261219676242 1109 1 0.46929405274975977 1109 2 0.2828921622395164 1109 3 0.35923894369078724 1109 4 0.4901013382902448 1109 5 0.5015564044515131 1109 6 0.4553039690427383 1109 7 0.43912323260303765 1109 8 0.37866820567749215 1109 9 0.23825846329428657 1109 10 0.2494675754904776 1109 11 0.23251824884503947 1109 12 0.18599233192154685 1109 13 0.258417904748768 1109 14 0.35055345358149026 1109 15 0.4180769379184355 1109 16 0.4185655895504965 1109 17 0.583314275383529 1109 18 0.621862193094195 1109 19 0.48678921234252925 1109 20 0.38803539913756424 1109 21 0.35056533104833676 1109 22 0.23037494255331836 1109 23 0.45545832705093403 1109 24 0.42025504126853974 1109 25 0.4955478179187338 1110 1 0.47012846174846956 1110 2 0.1542744690807467 1110 3 0.35004315722573054 1110 4 0.3095220924853379 1110 5 0.43927768418597424 1110 6 0.4364882766215672 1110 7 0.46977162832496333 1110 8 0.3271860924560315 1110 9 0.5020112292909644 1110 10 0.4381650215535373 1110 11 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16 0.30111328876486 2214 17 0.22171241803003738 2214 18 0.23055808446981985 2214 19 0.1332329525771243 2214 20 0.17841633265575751 2214 21 0.21140136018502176 2214 22 0.21586381823826883 2214 23 0.0287357637270631 2214 24 0.1299423280800056 2214 25 0.15571924900519463 2215 1 0.49524493370696376 2215 2 0.34854550462234 2215 3 0.23047961824291718 2215 4 0.454753580502846 2215 5 0.4163649146239834 2215 6 0.5308062980100559 2215 7 0.35862142361667537 2215 8 0.2984761958690072 2215 9 -0.003886266561927698 2215 10 0.447351526688891 2215 11 0.14350079390548842 2215 12 0.13384207384679314 2215 13 0.07653185173260368 2215 14 0.40354662814730347 2215 15 0.1493803385938577 2215 16 0.14669436967518124 2215 17 0.18974780813411485 2215 18 0.22114297733494226 2215 19 0.15163247913655198 2215 20 0.20596981585577367 2215 21 0.12136867425218867 2215 22 0.05658307724480238 2215 23 0.11963930231625852 2215 24 -0.00132581029605916 2215 25 0.07088430032969484 2216 1 -0.38310661426191217 2216 2 -0.21615332105644214 2216 3 -2.3187439210093466 2216 4 -1.8711717339885183 2216 5 -0.6469801949389762 2216 6 -0.4593357871529946 2216 7 -0.4454837480191216 2216 8 -0.31900875366929426 2216 9 -0.36714663129487235 2216 10 -0.6972979929069751 2216 11 -0.6345106741611133 2216 12 -0.5119146314503098 2216 13 -0.37152286921477895 2216 14 -0.3899962560384296 2216 15 -0.4114268283974809 2216 16 -0.6938488777026848 2216 17 -0.646753000793808 2216 18 -0.603777505600533 2216 19 -0.5887038587862108 2216 20 -0.6139771360818003 2216 21 -0.514318804424029 2216 22 -0.5516789933905599 2216 23 -0.6732289249759802 2216 24 -0.4994218030762383 2216 25 -0.6511372490699401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14867.0 3 7.0 4 225.0 5 1687.0 6 2257.0 7 1724.0 8 1278.0 9 1156.0 10 1040.0 11 916.0 12 852.0 13 786.0 14 802.0 15 738.0 16 812.0 17 843.0 18 949.0 19 1105.0 20 1501.0 21 1882.0 22 2363.0 23 2975.0 24 3478.0 25 4059.0 26 4998.0 27 5448.0 28 6149.0 29 7575.0 30 9750.0 31 12414.0 32 15964.0 33 21140.0 34 28764.0 35 39784.0 36 58398.0 37 100489.0 38 231599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.616336214200985 19.669208608710388 10.59769530588036 28.11675987120827 2 13.637765494148708 21.22953403171526 41.094105326659054 24.03859514747698 3 18.432836452593175 24.84048181348773 29.11670348832288 27.609978245596217 4 11.18549563792584 17.844725732682885 39.00814863213344 31.961629997257834 5 12.136601088123072 39.171753489679084 35.824437741010456 12.867207681187388 6 30.61840018205223 39.12781357308072 18.310344710465625 11.943441534401417 7 27.990999700328622 32.1555853157639 21.616633962417485 18.236781021489993 8 24.486171795779 38.85964927130434 19.925920032232234 16.728258900684427 9 25.79455231839651 16.19965803863148 20.168272079362122 37.83751756360989 10 13.091729623679848 31.38873324790477 35.05358469869504 20.46595242972034 11 33.93111363524793 23.721732535916757 23.822792052693142 18.524361776142168 12 21.809429018301476 26.554068236138477 32.32990053692457 19.306602208635482 13 28.098339788207895 22.749031768816184 25.993114048154265 23.159514394821656 14 19.647986620918694 23.365150404639344 28.68225189709076 28.3046110773512 15 22.26401424615239 31.688886782360232 23.831327993283182 22.2157709782042 16 20.8964925376924 30.523944215082377 27.931898931731354 20.647664315493873 17 20.499582750057975 29.82753104830451 28.235887557488237 21.436998644149273 18 20.011544275342814 29.74038922150621 29.753592351663677 20.4944741514873 19 21.71014100242057 28.45946815173418 28.85522284476192 20.975168001083333 20 21.6609791933267 28.873406322574528 29.474992044308124 19.99062243979065 21 21.94100827333319 28.050781031771155 28.34958260890175 21.658628085993907 22 21.06930907589027 29.381963322691927 28.699299562946234 20.849428038471565 23 21.027196571271876 28.710689215654746 28.51907585485457 21.743038358218808 24 20.719633429422128 28.58779937471753 29.762870002522117 20.92969719333822 25 21.24370487612889 28.630773527893215 28.92887673830059 21.1966448576773 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 209.0 2 226.0 3 243.0 4 243.0 5 243.0 6 418.0 7 593.0 8 593.0 9 593.0 10 935.5 11 1278.0 12 1278.0 13 1278.0 14 2213.0 15 3148.0 16 3148.0 17 3148.0 18 5289.0 19 7430.0 20 7430.0 21 7430.0 22 11942.5 23 16455.0 24 16455.0 25 16455.0 26 23037.0 27 29619.0 28 29619.0 29 29619.0 30 38501.0 31 47383.0 32 47383.0 33 47383.0 34 55061.5 35 62740.0 36 62740.0 37 62740.0 38 68194.0 39 73648.0 40 73648.0 41 73648.0 42 77499.0 43 81350.0 44 81350.0 45 81350.0 46 82133.5 47 82917.0 48 82917.0 49 82917.0 50 78071.5 51 73226.0 52 73226.0 53 73226.0 54 61883.0 55 50540.0 56 50540.0 57 50540.0 58 41045.5 59 31551.0 60 31551.0 61 31551.0 62 24162.5 63 16774.0 64 16774.0 65 16774.0 66 12169.0 67 7564.0 68 7564.0 69 7564.0 70 5158.5 71 2753.0 72 2753.0 73 2753.0 74 1854.0 75 955.0 76 955.0 77 955.0 78 623.5 79 292.0 80 292.0 81 292.0 82 190.0 83 88.0 84 88.0 85 88.0 86 52.0 87 16.0 88 16.0 89 16.0 90 9.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11408762064681248 2 0.10968661450910162 3 0.015064982548317968 4 0.0 5 0.005416622938721068 6 0.32702860992528443 7 0.021497222288049237 8 0.010325437476937035 9 0.010833245877442136 10 0.004570275604545901 11 0.005585892405556101 12 3.3853893367006676E-4 13 0.0010156168010102002 14 1.6926946683503338E-4 15 0.0030468504030306005 16 8.463473341751667E-4 17 1.6926946683503338E-4 18 8.463473341751667E-4 19 6.770778673401335E-4 20 3.3853893367006676E-4 21 0.013710826813637703 22 0.0 23 1.6926946683503338E-4 24 1.6926946683503338E-4 25 0.006601509206566301 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 590774.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.20597047113897 #Duplication Level Percentage of deduplicated Percentage of total 1 94.87798906836943 84.6368309119401 2 4.161720278968886 7.425005926296775 3 0.45884299754992586 1.2279460467098273 4 0.14916385622610878 0.5322522621546992 5 0.07821783959415249 0.34887491445761265 6 0.0483242605675701 0.2586487536738171 7 0.038217598415340434 0.23864665690016945 8 0.029372901989384234 0.20961905820133356 9 0.02259502647907942 0.1814050138398626 >10 0.11637807958925378 1.965511041845848 >50 0.010063790503430932 0.6040325796610242 >100 0.007405370170030225 1.1777369940520739 >500 0.0015190502910685107 0.9455095498679209 >1k 1.8988128638356384E-4 0.24798029039889577 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1464 0.24781049944648886 No Hit GGTATCAACGCAGAGTACTTTTTTT 961 0.16266795762846706 No Hit CACACACACACACACACACACACAC 878 0.14861859188115928 No Hit TATCAACGCAGAGTACTTTTTTTTT 805 0.13626192080220184 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 719 0.12170474665438899 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.6926946683503335E-4 0.0 7 0.0 0.0 0.0 1.6926946683503335E-4 0.0 8 0.0 0.0 0.0 1.6926946683503335E-4 0.0 9 0.0 0.0 0.0 1.6926946683503335E-4 0.0 10 0.0 0.0 0.0 1.6926946683503335E-4 0.0 11 0.0 0.0 0.0 1.6926946683503335E-4 0.0 12 0.0 0.0 0.0 1.6926946683503335E-4 0.0 13 0.0 0.0 0.0 1.6926946683503335E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 35 0.0021910337 16.260033 19 TCCAACG 50 8.8172616E-5 15.176031 18 GGACTAG 40 0.0051321043 14.30867 1 CGTTTCC 40 0.0053294827 14.227529 14 TAGGACC 225 0.0 13.549438 4 CTATTAC 65 5.2354546E-5 13.208003 1 ATAGGAC 110 2.6138878E-9 12.992398 3 GTTCTAG 75 1.41386845E-5 12.718818 1 CTTGTAC 75 1.4312976E-5 12.703678 3 GACGTGG 135 2.7284841E-11 12.650975 7 TTACACT 130 1.8735591E-10 12.458318 4 TAGCCCT 180 0.0 12.173324 4 GTGTTAC 55 0.0029716657 12.14069 1 TCTAGCC 55 0.0029983036 12.126239 3 GGACGTG 205 0.0 12.08296 6 CTAGAAC 95 1.0002386E-6 12.035063 3 CTAGGAC 95 1.0002386E-6 12.035063 3 CTGTAGG 430 0.0 11.979351 1 GTCCTAC 310 0.0 11.693107 1 GTAGGAC 410 0.0 11.619218 3 >>END_MODULE