FastQCFastQC Report
Thu 26 May 2016
SRR1926956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400562
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7890.19697325258012494No Hit
GGTATCAACGCAGAGTACTTTTTTT4350.10859742062402325No Hit
CACACACACACACACACACACACAC4300.10734917440995402No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG4170.10410373425337402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC551.0807478E-515.6101071
TCCAACG456.836256E-414.75213318
CGCCTTG653.2491207E-614.6614332
TCGCCTT400.005126840414.3092651
GTGCTAC400.005126840414.3092651
GTCTTAT602.4605868E-514.3092651
CCTAGAC400.00516113914.2948973
GGTACTG500.001474966613.3251815
TAATACT751.4418574E-512.692244
AGGACGT1700.012.3173955
ATAAGCC550.002990271412.1290043
GTAGGAC1900.012.0378093
GACGTGG802.8027474E-511.8959927
TAGGACG1800.011.6345534
AGACCAG1406.511982E-1011.5575565
TAGAGAG1001.8744377E-611.4215845
GGACGTG1501.6916601E-1011.4201536
CTGTAGG2300.011.1985551
CGTGGAA855.380651E-511.1598639
CCTATAC600.005726975411.1182553
Produced by FastQC (version 0.11.5)