##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926956_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 400562 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.376481044132994 30.0 26.0 31.0 2.0 31.0 2 26.02875210329487 30.0 26.0 31.0 2.0 33.0 3 25.67071764171339 30.0 26.0 31.0 2.0 34.0 4 29.289937637619143 35.0 28.0 37.0 2.0 37.0 5 28.76685257213615 35.0 28.0 35.0 2.0 37.0 6 28.60885705583655 35.0 28.0 36.0 2.0 37.0 7 28.625061788187598 35.0 28.0 36.0 2.0 37.0 8 28.326923172942017 35.0 27.0 36.0 2.0 37.0 9 30.4462080776509 37.0 30.0 39.0 2.0 39.0 10 30.68869238719599 37.0 30.0 39.0 2.0 39.0 11 30.87547745417688 37.0 30.0 39.0 2.0 39.0 12 30.760633809497655 37.0 30.0 39.0 2.0 39.0 13 30.85006565775086 37.0 30.0 39.0 2.0 39.0 14 31.67765289767876 38.0 31.0 40.0 2.0 41.0 15 31.240761729769673 38.0 30.0 40.0 2.0 41.0 16 31.282670348160835 38.0 30.0 40.0 2.0 41.0 17 31.49997004209086 38.0 31.0 40.0 2.0 41.0 18 31.531348455420133 38.0 31.0 40.0 2.0 41.0 19 30.918816562729365 38.0 29.0 40.0 2.0 41.0 20 31.21875514901563 38.0 30.0 40.0 2.0 41.0 21 31.174457387370744 38.0 30.0 40.0 2.0 41.0 22 31.17262745842092 38.0 30.0 40.0 2.0 41.0 23 31.12802013171494 38.0 30.0 40.0 2.0 41.0 24 29.856491629260887 37.0 27.0 40.0 2.0 41.0 25 30.37063925185115 38.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.107656725463329 1101 2 -1.1803986230446775 1101 3 -1.915734857469097 1101 4 -1.2642101870387776 1101 5 -1.3313317751688096 1101 6 -3.2621533199966564 1101 7 -2.0132727174074034 1101 8 -1.4305923304204207 1101 9 -1.4006458771617574 1101 10 -10.101698011526192 1101 11 -5.396814131104794 1101 12 -2.707286072821802 1101 13 -1.7077126890320464 1101 14 -1.6716516226441236 1101 15 -2.742343032277624 1101 16 -1.5961794532331837 1101 17 -1.4698901998443645 1101 18 -1.4446990010167546 1101 19 -1.8818249857212734 1101 20 -1.378698696341118 1101 21 -1.2710486968854333 1101 22 -0.7774205225971045 1101 23 -1.206899770772079 1101 24 -1.3075910124671282 1101 25 -1.2513978190591537 1102 1 -0.7306259131383968 1102 2 -0.43709716453023617 1102 3 -0.8428091354561538 1102 4 -0.3331950223522142 1102 5 -0.9144864163356203 1102 6 -1.6641623994905217 1102 7 -0.9649389011222667 1102 8 -0.279930686452488 1102 9 -0.02473551301610044 1102 10 0.10628035223310661 1102 11 0.08959560520352383 1102 12 0.12856826891489348 1102 13 -0.39773490481491436 1102 14 -0.5198888485689785 1102 15 0.23335483307541693 1102 16 0.051381640169694265 1102 17 -0.15734311204372986 1102 18 -0.5500984021565216 1102 19 -0.136205358560062 1102 20 0.12753138189707158 1102 21 0.2595028340717782 1102 22 0.019952219603222687 1102 23 -0.37332496153803874 1102 24 -3.506006930558506 1102 25 -2.1142201893865966 1103 1 -1.0500709004356423 1103 2 -0.9251956597422755 1103 3 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0.5429755050932812 1104 15 0.6530625181296337 1104 16 0.22759430345282894 1104 17 0.3043786063465994 1104 18 0.43694433573071123 1104 19 -0.7297821952353267 1104 20 0.0860137847592064 1104 21 -0.2464100261218043 1104 22 0.3321162931534367 1104 23 0.301342925418723 1104 24 1.723623931687385 1104 25 1.0531592376317356 1105 1 0.2995128839534722 1105 2 0.12415295349450162 1105 3 0.8974420211862792 1105 4 0.6523965176345676 1105 5 0.3443937649503752 1105 6 0.26785535143007166 1105 7 0.715846671533761 1105 8 1.1510390047947965 1105 9 0.43456011534760464 1105 10 0.9661236153810613 1105 11 0.4387977579747435 1105 12 0.4575079346688362 1105 13 0.5339618188183621 1105 14 0.3407097729020805 1105 15 0.7158588459165074 1105 16 0.15936011245748816 1105 17 0.40496450763335545 1105 18 0.35098742543294037 1105 19 -0.4494713391095857 1105 20 0.30315219201887444 1105 21 0.5448772085406652 1105 22 0.6295830382228047 1105 23 0.3180167436833763 1105 24 -3.0988310264890124 1105 25 -0.9072897767721102 1106 1 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1107 13 -0.5827178853488988 1107 14 -0.23888416821582226 1107 15 -1.5783992356406777 1107 16 -0.977381257394395 1107 17 -0.7856911601237186 1107 18 -0.9496277075495634 1107 19 -0.1398273136712227 1107 20 -0.03859456215143098 1107 21 -0.37938930506235735 1107 22 -0.420734941337102 1107 23 -0.7369033042675355 1107 24 -4.78811521691102 1107 25 -2.528779810703476 1108 1 0.290170366538522 1108 2 0.06192924214893836 1108 3 0.555657047133387 1108 4 0.4866467007447923 1108 5 0.32097903197962907 1108 6 -2.0907757632826396 1108 7 -1.2084677689800891 1108 8 0.19217776858000946 1108 9 0.033935722468385165 1108 10 0.1637060409551978 1108 11 0.1984558483298997 1108 12 0.5583172558883227 1108 13 0.24606319695006462 1108 14 0.2633125873451938 1108 15 -0.764749651436837 1108 16 0.284693217227705 1108 17 -0.07283629961001736 1108 18 -0.054452376756071175 1108 19 0.14015916783301918 1108 20 -1.386542208704217 1108 21 -0.2610051749570772 1108 22 -0.03492526537134921 1108 23 -0.1243515857799693 1108 24 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0.2834225699516857 1110 12 0.5150858948331845 1110 13 0.3760931169598365 1110 14 0.302284926601466 1110 15 0.7489433326261796 1110 16 0.4857984257518524 1110 17 0.16397447159350165 1110 18 0.5828438313406537 1110 19 -2.0142799846369073 1110 20 0.02768200818532307 1110 21 -0.037368367238936884 1110 22 0.1134382932779765 1110 23 0.1060630200696977 1110 24 -1.444770737968259 1110 25 -0.8222377360278941 1111 1 1.2444379098591547 1111 2 0.8298770935274149 1111 3 1.5983939280477273 1111 4 1.428947302932329 1111 5 1.3080750132995256 1111 6 1.4975092311870668 1111 7 1.8468710127166865 1111 8 2.137806788284241 1111 9 1.271671784079956 1111 10 1.3101256878357006 1111 11 1.0675261445078519 1111 12 1.0408302623198509 1111 13 1.1388011019928719 1111 14 0.7927614014044266 1111 15 0.26495519473906626 1111 16 0.4118592489347215 1111 17 0.5196885867117231 1111 18 0.8684102671339815 1111 19 1.2620714205059969 1111 20 0.6402029062029975 1111 21 1.1601676569629618 1111 22 1.0428726535996091 1111 23 1.223539733707149 1111 24 1.5599860489117638 1111 25 1.3857771513218395 1112 1 1.5579747445159668 1112 2 1.6811527441288767 1112 3 0.09827599424790279 1112 4 1.5170241957698174 1112 5 1.632864698023166 1112 6 2.515779142343604 1112 7 2.334714003517849 1112 8 2.987716372371043 1112 9 1.7396232739769566 1112 10 2.1989730814987283 1112 11 2.2195919515681553 1112 12 2.2704571983997326 1112 13 1.7828277550316365 1112 14 2.1719087400316823 1112 15 2.484597069100257 1112 16 2.2737784249705726 1112 17 1.4689082580695327 1112 18 1.7770115722680302 1112 19 2.6187818537161505 1112 20 2.243168941268646 1112 21 2.1929335976815665 1112 22 2.191380791031797 1112 23 2.3335621411291143 1112 24 3.3983774318821247 1112 25 2.443397640353993 1113 1 -0.17882528649148455 1113 2 -0.15504312606617887 1113 3 -4.6033330017889575 1113 4 -1.1345610601870035 1113 5 0.45686349648442004 1113 6 0.8236782906076705 1113 7 0.9055732975169377 1113 8 1.2903127619409105 1113 9 0.38480839815211354 1113 10 0.8410356584117409 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1.323170085667222 2215 3 1.4745763703640478 2215 4 1.7483188775066516 2215 5 2.3154963626002996 2215 6 2.6858783872401943 2215 7 1.9712810105712215 2215 8 1.0669041337621934 2215 9 1.3622127331544789 2215 10 1.1061068467754538 2215 11 1.2359668809865667 2215 12 0.7398487132960341 2215 13 1.0550573677181383 2215 14 1.259236114205038 2215 15 1.1088996272642753 2215 16 1.2460003813487575 2215 17 0.798586501712407 2215 18 0.4078770222197008 2215 19 1.3834460767444128 2215 20 -1.6835936844622914 2215 21 -0.6440540278101103 2215 22 0.3930096706267747 2215 23 0.6514448847672014 2215 24 0.6545814342172989 2215 25 0.5968491998482968 2216 1 0.39168725848357155 2216 2 -0.7029865997208447 2216 3 0.06977956290811704 2216 4 0.035670319570616726 2216 5 0.5107400113505278 2216 6 -1.589131414649259 2216 7 -8.797721544163277 2216 8 -9.696759200678276 2216 9 -5.265120678427483 2216 10 -2.9549021110277245 2216 11 -2.3022679797766585 2216 12 -1.9273215748670296 2216 13 -1.6483811600755267 2216 14 -1.5584762122016258 2216 15 -3.1804446575474152 2216 16 -2.0757338413551665 2216 17 -2.1450145501489537 2216 18 -1.810559020763769 2216 19 -1.22926066820418 2216 20 -2.1079942708275503 2216 21 -4.645142849160997 2216 22 -3.3101241869723736 2216 23 -3.0415624348093573 2216 24 -3.231594280571443 2216 25 -2.9329173278854306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42996.0 3 7.0 4 338.0 5 1675.0 6 2003.0 7 1585.0 8 1287.0 9 1129.0 10 1104.0 11 1134.0 12 1061.0 13 1050.0 14 1036.0 15 1068.0 16 1151.0 17 1233.0 18 1411.0 19 1780.0 20 2273.0 21 2921.0 22 3452.0 23 4099.0 24 4769.0 25 5272.0 26 5888.0 27 6479.0 28 7118.0 29 8230.0 30 9805.0 31 11797.0 32 14927.0 33 19014.0 34 25218.0 35 34376.0 36 46976.0 37 67058.0 38 57842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69838923118873 18.333595999469665 12.765073909247599 25.20294086009401 2 13.913465095122415 20.08642493676834 42.62088994191684 23.379220026192403 3 20.347654395041364 24.353552772987427 28.478918297468198 26.819874534503015 4 11.058123471470362 16.706594754473485 39.14559721011334 33.08968456394281 5 12.086707468418595 38.31943726543363 35.789179295483095 13.80467597066469 6 30.432828441727715 37.43289336349699 19.863814104004675 12.270464090770622 7 27.57038472225351 31.3069642512948 22.9511643799611 18.17148664649059 8 25.47925522061511 37.25398899552912 20.702473752985178 16.564282030870594 9 25.988988351623654 16.089491491566477 20.367054945878124 37.554465210931745 10 13.374591971548522 30.883539251899972 35.3945169967108 20.347351779840707 11 34.06874923532893 23.751869542745425 23.458735227803317 18.720645994122332 12 21.994087302181807 26.41288009308504 32.18999535573567 19.40303724899749 13 28.07940074906367 22.718352059925092 25.805493133583024 23.396754057428215 14 19.65887299748312 23.237185889497024 28.879938875794014 28.224002237225843 15 23.107250400747063 31.274001128583627 23.60162995440722 22.01711851626209 16 20.81310800822996 30.239357983260422 28.370388126485686 20.57714588202393 17 20.755970156707253 29.573374749058658 28.166605075756816 21.504050018477272 18 20.131185034818717 29.578305281109007 29.727367834947554 20.563141849124726 19 22.425925278544927 28.19359059514475 28.467016585346368 20.913467540963957 20 21.906835523950303 28.549348807478726 29.47694858375614 20.06686708481483 21 22.387653494863947 27.815341746108636 28.18412891820765 21.612875840819772 22 21.36880610244822 29.10573165707437 28.77663890934968 20.74882333112773 23 21.2654871234026 28.697308823602853 28.429639395346744 21.607564657647803 24 21.760245292770804 28.292313876869223 29.05960284743359 20.887837982926385 25 21.36038931044212 28.582058061987052 28.914390154380865 21.143162473189964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 174.0 1 174.0 2 224.0 3 274.0 4 274.0 5 274.0 6 389.0 7 504.0 8 504.0 9 504.0 10 769.0 11 1034.0 12 1034.0 13 1034.0 14 1601.0 15 2168.0 16 2168.0 17 2168.0 18 3652.5 19 5137.0 20 5137.0 21 5137.0 22 8034.5 23 10932.0 24 10932.0 25 10932.0 26 15271.0 27 19610.0 28 19610.0 29 19610.0 30 25483.0 31 31356.0 32 31356.0 33 31356.0 34 36248.5 35 41141.0 36 41141.0 37 41141.0 38 44997.5 39 48854.0 40 48854.0 41 48854.0 42 51600.5 43 54347.0 44 54347.0 45 54347.0 46 55210.0 47 56073.0 48 56073.0 49 56073.0 50 52940.0 51 49807.0 52 49807.0 53 49807.0 54 42660.0 55 35513.0 56 35513.0 57 35513.0 58 28863.5 59 22214.0 60 22214.0 61 22214.0 62 17114.0 63 12014.0 64 12014.0 65 12014.0 66 8815.0 67 5616.0 68 5616.0 69 5616.0 70 3991.0 71 2366.0 72 2366.0 73 2366.0 74 1623.0 75 880.0 76 880.0 77 880.0 78 603.0 79 326.0 80 326.0 81 326.0 82 235.0 83 144.0 84 144.0 85 144.0 86 94.0 87 44.0 88 44.0 89 44.0 90 31.5 91 19.0 92 19.0 93 19.0 94 13.5 95 8.0 96 8.0 97 8.0 98 7.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20346413289328494 2 0.11134356229497556 3 0.11284145775185865 4 0.06465915388878626 5 0.022218782610432343 6 0.019971939425107724 7 0.2693715329961404 8 0.27161837618146506 9 0.019222991696666185 10 0.04019352809302929 11 0.015727902297272334 12 0.016976148511341566 13 0.015478253054458487 14 0.016476850025713873 15 0.014978954568830794 16 0.018474043968224645 17 0.01747544699696926 18 0.014729305326016948 19 0.021969133367618496 20 0.016476850025713873 21 0.014978954568830794 22 0.01672649926852772 23 0.016976148511341566 24 0.01522860381164464 25 0.014729305326016948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 400562.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.39144566818418 #Duplication Level Percentage of deduplicated Percentage of total 1 96.82654101710892 89.45944103620424 2 2.4868623148251503 4.595296088888451 3 0.3044051744234534 0.8437330240157584 4 0.11291368149533654 0.41729033076284155 5 0.0667544740505608 0.3083771181175299 6 0.035446740589154487 0.19649853643542511 7 0.033438742355036966 0.21626176232554645 8 0.01748091341440757 0.12920694895659748 9 0.012715809478136351 0.10573488184735944 >10 0.08641318404435626 1.5421103738353172 >50 0.010811395816901895 0.6824850896542978 >100 0.005946267511706915 1.3065356386748288 >500 2.7028488689576844E-4 0.19702917028181177 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 789 0.19697325258012494 No Hit GGTATCAACGCAGAGTACTTTTTTT 435 0.10859742062402325 No Hit CACACACACACACACACACACACAC 430 0.10734917440995402 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 417 0.10410373425337402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 2.4964924281384657E-4 3 0.0 0.0 0.0 0.0 2.4964924281384657E-4 4 0.0 0.0 0.0 0.0 2.4964924281384657E-4 5 0.0 0.0 0.0 0.0 2.4964924281384657E-4 6 0.0 0.0 0.0 0.0 2.4964924281384657E-4 7 0.0 0.0 0.0 0.0 2.4964924281384657E-4 8 0.0 0.0 0.0 0.0 2.4964924281384657E-4 9 0.0 0.0 0.0 0.0 2.4964924281384657E-4 10 0.0 0.0 0.0 0.0 2.4964924281384657E-4 11 0.0 0.0 0.0 0.0 2.4964924281384657E-4 12 0.0 0.0 0.0 0.0 2.4964924281384657E-4 13 0.0 0.0 0.0 0.0 2.4964924281384657E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCTAC 55 1.0807478E-5 15.610107 1 TCCAACG 45 6.836256E-4 14.752133 18 CGCCTTG 65 3.2491207E-6 14.661433 2 TCGCCTT 40 0.0051268404 14.309265 1 GTGCTAC 40 0.0051268404 14.309265 1 GTCTTAT 60 2.4605868E-5 14.309265 1 CCTAGAC 40 0.005161139 14.294897 3 GGTACTG 50 0.0014749666 13.325181 5 TAATACT 75 1.4418574E-5 12.69224 4 AGGACGT 170 0.0 12.317395 5 ATAAGCC 55 0.0029902714 12.129004 3 GTAGGAC 190 0.0 12.037809 3 GACGTGG 80 2.8027474E-5 11.895992 7 TAGGACG 180 0.0 11.634553 4 AGACCAG 140 6.511982E-10 11.557556 5 TAGAGAG 100 1.8744377E-6 11.421584 5 GGACGTG 150 1.6916601E-10 11.420153 6 CTGTAGG 230 0.0 11.198555 1 CGTGGAA 85 5.380651E-5 11.159863 9 CCTATAC 60 0.0057269754 11.118255 3 >>END_MODULE