FastQCFastQC Report
Thu 26 May 2016
SRR1926956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400562
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13370.3337810376421128No Hit
GGTATCAACGCAGAGTACTTTTTTT9430.2354192359734573No Hit
TATCAACGCAGAGTACTTTTTTTTT6560.16376990328588334No Hit
CACACACACACACACACACACACAC6320.15777832145835102No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG4960.1238260244356679No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4890.12207847973597097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTG451.6169834E-619.0546655
ATTAGGT350.002125218316.332573
TCCAACG350.00218831716.26121318
ATAGGAC601.4170655E-615.8788853
GGCTTAC456.557547E-414.8333131
TAGGACT456.601371E-414.8202944
ATCAGAC400.005170503614.29099753
CGTGTGC400.00532295814.2285610
GTCCTAG903.4384357E-813.773791
CCTAGAC500.001464096513.3382643
AGACTGT655.2881995E-513.1916916
TAGGACC1450.013.1411484
GTATAGG603.953076E-412.7142671
GTCCTAC2450.012.4547931
GTAGGAC2250.012.2796723
GTATAAC550.002976747212.1363471
CTAGACA550.002996398412.1256954
ACTGATC550.003093437512.0742258
TAGACAG1052.601355E-711.7957445
GGTATCA3950.011.58768751
Produced by FastQC (version 0.11.5)