##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926956_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 400562 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.641785790963695 31.0 30.0 33.0 25.0 34.0 2 29.97026677518087 31.0 30.0 34.0 26.0 34.0 3 30.040458156290413 31.0 30.0 34.0 26.0 34.0 4 33.59111198765734 35.0 35.0 37.0 28.0 37.0 5 33.55037422421498 35.0 35.0 37.0 28.0 37.0 6 33.473981555913944 37.0 35.0 37.0 28.0 37.0 7 33.4502274304602 37.0 35.0 37.0 28.0 37.0 8 33.4684418392159 37.0 35.0 37.0 28.0 37.0 9 34.9612020111743 39.0 35.0 39.0 28.0 39.0 10 34.8643356084701 39.0 35.0 39.0 28.0 39.0 11 34.88000858793395 39.0 35.0 39.0 28.0 39.0 12 34.75694649018129 39.0 35.0 39.0 27.0 39.0 13 34.7811325088251 39.0 35.0 39.0 27.0 39.0 14 35.84582911010031 40.0 36.0 41.0 27.0 41.0 15 35.82360034152016 40.0 36.0 41.0 27.0 41.0 16 35.78998007799042 40.0 36.0 41.0 27.0 41.0 17 35.769296638223295 40.0 36.0 41.0 27.0 41.0 18 35.74870806516844 40.0 36.0 41.0 27.0 41.0 19 35.827981685731544 40.0 36.0 41.0 27.0 41.0 20 35.78858703521552 40.0 36.0 41.0 27.0 41.0 21 35.736003415201644 40.0 36.0 41.0 27.0 41.0 22 35.645363264613216 40.0 36.0 41.0 27.0 41.0 23 35.56979443881347 40.0 36.0 41.0 27.0 41.0 24 35.48794194157209 40.0 36.0 41.0 27.0 41.0 25 35.37814620458256 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21826744999020065 1101 2 0.14040364398325522 1101 3 0.4200183847151848 1101 4 0.2620149696285523 1101 5 0.09508075434894891 1101 6 0.20584940252982165 1101 7 0.36150070865591033 1101 8 0.48131032572089083 1101 9 0.40270256533498383 1101 10 0.2761575700085004 1101 11 0.30654966009953455 1101 12 0.3091481414204509 1101 13 0.3083017313301255 1101 14 0.44946614018951436 1101 15 0.6106337199532206 1101 16 0.5326231538691317 1101 17 0.5834964012300787 1101 18 0.47406032358891537 1101 19 0.6451311077969137 1101 20 0.5055990482061361 1101 21 0.16909105649284584 1101 22 0.497703032331664 1101 23 0.6393058514888423 1101 24 0.4641857944151937 1101 25 0.6991944617765782 1102 1 -0.4421594794022461 1102 2 -0.5295084587888894 1102 3 -0.19964741263823882 1102 4 -0.3941813019835365 1102 5 -0.5041465227687922 1102 6 -0.2866841626203751 1102 7 0.11262598635319421 1102 8 0.16779733431743438 1102 9 -0.07176860244247507 1102 10 0.06995646887838802 1102 11 0.04252956930459817 1102 12 -0.010662541473394072 1102 13 0.21744883844883844 1102 14 0.13506451747146286 1102 15 0.08947077201542442 1102 16 -0.16450811243048946 1102 17 0.08885716622572915 1102 18 -0.0407469786423178 1102 19 -0.09780330734845677 1102 20 0.22695517686450017 1102 21 0.14094462550665554 1102 22 -0.08481411245728765 1102 23 -0.011307497298950864 1102 24 0.009689513144060413 1102 25 0.14640782988244183 1103 1 -0.9262126355484241 1103 2 -0.6291957755760684 1103 3 -0.4933546629802379 1103 4 -0.5672514602794152 1103 5 -0.5473945196419621 1103 6 -0.54918171978278 1103 7 -0.6659747562694349 1103 8 -0.7076765761770005 1103 9 -0.5867578539570317 1103 10 -0.7177707150184247 1103 11 -0.7101380093547718 1103 12 -0.5922533173186153 1103 13 -0.5796197564309722 1103 14 -0.4924516052566972 1103 15 -0.6867848465110526 1103 16 -0.7778362343769416 1103 17 -0.68005039679948 1103 18 -0.686496441218047 1103 19 -0.527664554172766 1103 20 -0.6071859214352884 1103 21 -0.8938218392187665 1103 22 -0.7270458888887887 1103 23 -0.9372015758606054 1103 24 -0.8891574675086673 1103 25 -0.6861112242508369 1104 1 -0.029948258280132478 1104 2 0.008886305057245636 1104 3 0.3215856350362749 1104 4 -0.04907771599469157 1104 5 0.33734611388982927 1104 6 0.06544718496816415 1104 7 -0.06533113709024008 1104 8 -0.3014125278452511 1104 9 -0.029632540096741877 1104 10 0.18586184092820446 1104 11 -0.011257490073610654 1104 12 -0.032184021352478 1104 13 -0.21281712932440655 1104 14 0.013562451298462008 1104 15 -0.19420784794741053 1104 16 -5.427242950375444E-4 1104 17 0.04225040470924313 1104 18 -0.10914701192295695 1104 19 -0.158044037303803 1104 20 -0.4038044537204115 1104 21 -0.2572693135343016 1104 22 -0.307081633327023 1104 23 -0.33197477411066245 1104 24 -0.2937439394448944 1104 25 -0.24136708206253132 1105 1 0.09620328143242673 1105 2 0.032213758187790376 1105 3 0.1675322682228675 1105 4 0.15196164957910696 1105 5 0.07829139621902215 1105 6 0.11926428417407209 1105 7 -0.12954189203411204 1105 8 -0.33644211427099435 1105 9 -0.3223209582162454 1105 10 -0.16735823228297164 1105 11 -0.32251622429374294 1105 12 -0.046730901401637936 1105 13 -0.13462367595018065 1105 14 0.11089305113211623 1105 15 0.18702529944437885 1105 16 0.03951417064003948 1105 17 0.1618436641957075 1105 18 0.21613150308745332 1105 19 0.1781102041235485 1105 20 -0.03405889168426768 1105 21 -0.09134694280606226 1105 22 -0.021401781232661676 1105 23 -0.12776486676359156 1105 24 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1107 11 0.7193112946529681 1107 12 0.48134628737459906 1107 13 0.367560528694554 1107 14 0.38703239322936156 1107 15 0.37533929664046894 1107 16 -4.6439367716999413E-4 1107 17 0.18364761405042884 1107 18 0.3120053881271758 1107 19 0.22775514388013818 1107 20 0.4424482095205988 1107 21 0.4442022576734459 1107 22 0.413361613529311 1107 23 0.2546588892556727 1107 24 -0.04160290255639865 1107 25 0.12458834259386009 1108 1 0.6410120416333527 1108 2 0.46580578341284706 1108 3 0.6173496774599343 1108 4 0.7677860092127844 1108 5 0.8209085478031213 1108 6 0.6415310069425928 1108 7 0.6300772386987958 1108 8 0.5961811150141756 1108 9 0.7926462897279904 1108 10 0.394535620927563 1108 11 0.5861182669195628 1108 12 0.7647175134611999 1108 13 0.5241497712491423 1108 14 0.7188397751473516 1108 15 0.649795484424665 1108 16 0.8178910608310446 1108 17 0.6918867275648282 1108 18 0.7091265630073522 1108 19 0.7263162433844599 1108 20 0.9381161461065659 1108 21 0.819775769614786 1108 22 1.0577330034764643 1108 23 0.7894485332686187 1108 24 0.7278043318870928 1108 25 0.9466716247207643 1109 1 0.5326436702040525 1109 2 0.46071188131565677 1109 3 0.500809147938746 1109 4 0.5557558382268724 1109 5 0.22939413758983562 1109 6 0.40961703502403424 1109 7 0.5468878800870698 1109 8 -0.029145694862741323 1109 9 0.33544373130992966 1109 10 0.2518528494778778 1109 11 0.25215042552110134 1109 12 0.3039143128903987 1109 13 0.2535766754103932 1109 14 0.3639535534007976 1109 15 0.4799690185581369 1109 16 0.4872486532679119 1109 17 0.37450528374045433 1109 18 0.21737516785407252 1109 19 0.5080327278530277 1109 20 0.7153426578411413 1109 21 0.5521535185126822 1109 22 0.3975388287191848 1109 23 0.5408793616966179 1109 24 0.7549684121052209 1109 25 0.520972773970314 1110 1 0.5056392627361177 1110 2 0.23835264791794586 1110 3 0.5251319330623048 1110 4 0.6420456416240938 1110 5 0.5269749824370606 1110 6 0.4554621298588799 1110 7 0.2843637380576496 1110 8 0.45925127438402313 1110 9 0.34445675562528066 1110 10 0.3319325800775843 1110 11 0.33221034727616683 1110 12 -0.07666063882554397 1110 13 0.22211032568524303 1110 14 0.18440123328986857 1110 15 0.3427184861954373 1110 16 0.3987972096983583 1110 17 0.3019801512688076 1110 18 -0.012083060244769683 1110 19 0.2402840030473996 1110 20 0.19567247510454422 1110 21 0.062753726505548 1110 22 0.31735014947390283 1110 23 0.2766714678720277 1110 24 0.426054927234226 1110 25 0.49294078268620467 1111 1 0.15214431806611017 1111 2 0.26650526315558665 1111 3 0.28813846120496933 1111 4 0.33796953303401267 1111 5 0.35473551105739176 1111 6 0.17627456517453766 1111 7 0.31729304304928974 1111 8 -0.04316692480393414 1111 9 -0.03195634842694517 1111 10 0.041738726085071676 1111 11 0.2066530668048614 1111 12 -0.05188156072666317 1111 13 -0.14351542067252865 1111 14 -0.16676507512507044 1111 15 0.06837727132401028 1111 16 0.13719383417093667 1111 17 0.017508373544103506 1111 18 0.07941045141831182 1111 19 0.23724881476621107 1111 20 0.29529631078641927 1111 21 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2214 14 0.12526478919691897 2214 15 -0.09280668986752971 2214 16 -0.049306582716809544 2214 17 -0.03890411792864512 2214 18 -0.008477064688712233 2214 19 -0.06907840421354905 2214 20 0.023968712817797666 2214 21 -0.033958509400001446 2214 22 -0.006652788047283309 2214 23 -0.044742308349114523 2214 24 -0.24239119938047082 2214 25 -0.007972485117811345 2215 1 1.0835724158211768 2215 2 1.0741377454555234 2215 3 0.936202181436677 2215 4 0.9938520007057932 2215 5 1.0331843417804123 2215 6 1.2481451157781933 2215 7 1.000639866789811 2215 8 1.0751480095644226 2215 9 1.1355238060463506 2215 10 1.0253966418659246 2215 11 0.5832036509557454 2215 12 1.1026209845718427 2215 13 1.2995298680871983 2215 14 1.3397103203226521 2215 15 1.062282207837832 2215 16 0.9285866293182892 2215 17 1.1807244216024557 2215 18 1.056980617789165 2215 19 1.1335571567139056 2215 20 1.1450139874457435 2215 21 1.3770907326398074 2215 22 1.2557822801829772 2215 23 1.2411766088242402 2215 24 1.4242354856162578 2215 25 1.361347426676474 2216 1 -0.4168758489322286 2216 2 -0.45084645914300125 2216 3 -2.183870340058803 2216 4 -1.8556633988741602 2216 5 -1.206109123043916 2216 6 -0.46045532424547986 2216 7 -0.2823238250138118 2216 8 -0.2530816817946828 2216 9 -0.11541705047646644 2216 10 -0.238832330271741 2216 11 -0.27706510811255924 2216 12 -0.3479138115113969 2216 13 -0.36193909176376593 2216 14 -0.2932345168910686 2216 15 -0.14038090933782854 2216 16 -0.22491970008013595 2216 17 -0.5051256456996853 2216 18 -0.35987313802016985 2216 19 -0.43979968450547346 2216 20 -0.482089672842001 2216 21 -0.4338289468071963 2216 22 -0.30262324583510747 2216 23 -0.6233800086373549 2216 24 -0.6203937032867586 2216 25 -0.8632894039543686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10927.0 3 7.0 4 182.0 5 1258.0 6 1747.0 7 1267.0 8 1002.0 9 855.0 10 805.0 11 673.0 12 624.0 13 599.0 14 576.0 15 572.0 16 589.0 17 621.0 18 626.0 19 776.0 20 1100.0 21 1367.0 22 1713.0 23 2047.0 24 2411.0 25 2901.0 26 3359.0 27 3742.0 28 4297.0 29 5272.0 30 6677.0 31 8595.0 32 11021.0 33 14725.0 34 19353.0 35 26931.0 36 39583.0 37 67895.0 38 153867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.394953047242694 19.393225905624583 10.19938362789721 28.012437419235507 2 13.814482453575632 21.082947869264583 40.85119462624354 24.251375050916245 3 18.891069431128155 24.560562480025567 28.840084691594758 27.70828339725152 4 11.221234165996774 17.73508220949566 38.40304372356839 32.64063990093918 5 12.058380748134407 39.350331924412615 35.62763548482935 12.963651842623639 6 30.737393805320817 38.767978875056684 18.176420334261138 12.318206985361355 7 28.21015413733779 32.37782999198476 20.83887866522509 18.573137205452362 8 24.823298837804437 38.721959379330144 19.504912179959305 16.949829602906114 9 25.974648527322614 16.293202240037548 19.76560998080039 37.96653925183944 10 13.41039307960205 31.501042303803473 34.40301581555592 20.685548801038557 11 34.14786983061003 23.95501242026688 23.156869843092707 18.740247906030383 12 21.86378138715105 26.77687848572755 31.724926478297995 19.634413648823404 13 28.083363083275703 22.628014210070376 25.864475073709126 23.424147632944795 14 19.65638278219102 23.504476210923652 28.48847369445928 28.350667312426044 15 22.577005077916308 31.560972443441397 23.575861672966212 22.28616080567608 16 20.98681599057322 30.491236041451863 27.86292225154221 20.6590257164327 17 20.38460962347901 29.897494020900634 28.02462540131116 21.693270954309195 18 19.980227730607822 29.61271816277671 29.81917860201068 20.587875504604792 19 21.616129338279716 28.56012302714686 28.829993858628626 20.9937537759448 20 21.679095069165495 28.756668772047206 29.57701823192972 19.987217926857582 21 22.034131056265057 27.752768310799848 28.457361147522565 21.75573948541253 22 21.090867331399384 29.36199639506493 28.71989854254772 20.827237730987964 23 21.06864854878895 28.5933263764411 28.650745702288283 21.68727937248166 24 20.8224943953745 28.307477993419244 29.969642652073837 20.90038495913242 25 21.202641467037836 28.49488808718339 29.05189309298813 21.250577352790643 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 209.5 3 206.0 4 206.0 5 206.0 6 306.5 7 407.0 8 407.0 9 407.0 10 695.0 11 983.0 12 983.0 13 983.0 14 1538.0 15 2093.0 16 2093.0 17 2093.0 18 3466.5 19 4840.0 20 4840.0 21 4840.0 22 7813.0 23 10786.0 24 10786.0 25 10786.0 26 15035.0 27 19284.0 28 19284.0 29 19284.0 30 25118.0 31 30952.0 32 30952.0 33 30952.0 34 36188.5 35 41425.0 36 41425.0 37 41425.0 38 45254.0 39 49083.0 40 49083.0 41 49083.0 42 51716.0 43 54349.0 44 54349.0 45 54349.0 46 55658.0 47 56967.0 48 56967.0 49 56967.0 50 53864.5 51 50762.0 52 50762.0 53 50762.0 54 43147.0 55 35532.0 56 35532.0 57 35532.0 58 29099.5 59 22667.0 60 22667.0 61 22667.0 62 17179.0 63 11691.0 64 11691.0 65 11691.0 66 8490.0 67 5289.0 68 5289.0 69 5289.0 70 3652.5 71 2016.0 72 2016.0 73 2016.0 74 1366.5 75 717.0 76 717.0 77 717.0 78 460.5 79 204.0 80 204.0 81 204.0 82 135.0 83 66.0 84 66.0 85 66.0 86 43.0 87 20.0 88 20.0 89 20.0 90 14.0 91 8.0 92 8.0 93 8.0 94 5.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1195819873078325 2 0.09861145091146939 3 0.012482462140692329 4 0.0 5 0.004743335613463085 6 0.35275438009596516 7 0.018723693211038492 8 0.0067405295559738575 9 0.008238425012856937 10 0.001747544699696926 11 0.001747544699696926 12 0.0 13 2.4964924281384657E-4 14 0.0 15 9.985969712553863E-4 16 2.4964924281384657E-4 17 0.0 18 2.4964924281384657E-4 19 0.0 20 2.4964924281384657E-4 21 0.011733514412250788 22 0.0 23 0.0 24 0.0 25 0.0067405295559738575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 400562.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.7448716105311 #Duplication Level Percentage of deduplicated Percentage of total 1 95.51043638471214 85.7157185081179 2 3.616272688218056 6.490838562255992 3 0.39480065558795857 1.0629400244248457 4 0.13615759657678364 0.4887778409432775 5 0.07661093916182503 0.34377194495200986 6 0.051397216384900295 0.2767581951360938 7 0.03894843943934902 0.24467958878404464 8 0.030979384568782516 0.22241927125589245 9 0.020164625592764607 0.16287045614073806 >10 0.1027883453939059 1.7938656815891523 >50 0.012253556903141206 0.7692858583742411 >100 0.00807620776824841 1.536322315610343 >500 8.354697691291457E-4 0.5575947756835326 >1k 2.7848992304304855E-4 0.33415697673190636 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1337 0.3337810376421128 No Hit GGTATCAACGCAGAGTACTTTTTTT 943 0.2354192359734573 No Hit TATCAACGCAGAGTACTTTTTTTTT 656 0.16376990328588334 No Hit CACACACACACACACACACACACAC 632 0.15777832145835102 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 496 0.1238260244356679 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 489 0.12207847973597097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.4964924281384657E-4 0.0 0.0 0.0 6 0.0 2.4964924281384657E-4 0.0 0.0 0.0 7 0.0 2.4964924281384657E-4 0.0 0.0 0.0 8 0.0 2.4964924281384657E-4 0.0 0.0 0.0 9 0.0 2.4964924281384657E-4 0.0 0.0 0.0 10 0.0 2.4964924281384657E-4 0.0 0.0 0.0 11 0.0 2.4964924281384657E-4 0.0 0.0 0.0 12 0.0 2.4964924281384657E-4 0.0 0.0 0.0 13 0.0 2.4964924281384657E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTG 45 1.6169834E-6 19.054665 5 ATTAGGT 35 0.0021252183 16.33257 3 TCCAACG 35 0.002188317 16.261213 18 ATAGGAC 60 1.4170655E-6 15.878885 3 GGCTTAC 45 6.557547E-4 14.833313 1 TAGGACT 45 6.601371E-4 14.820294 4 ATCAGAC 40 0.0051705036 14.2909975 3 CGTGTGC 40 0.005322958 14.22856 10 GTCCTAG 90 3.4384357E-8 13.77379 1 CCTAGAC 50 0.0014640965 13.338264 3 AGACTGT 65 5.2881995E-5 13.191691 6 TAGGACC 145 0.0 13.141148 4 GTATAGG 60 3.953076E-4 12.714267 1 GTCCTAC 245 0.0 12.454793 1 GTAGGAC 225 0.0 12.279672 3 GTATAAC 55 0.0029767472 12.136347 1 CTAGACA 55 0.0029963984 12.125695 4 ACTGATC 55 0.0030934375 12.074225 8 TAGACAG 105 2.601355E-7 11.795744 5 GGTATCA 395 0.0 11.5876875 1 >>END_MODULE