FastQCFastQC Report
Thu 26 May 2016
SRR1926955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586191
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8790.1499511251452172No Hit
GTCCTACAGTGGACATTTCTAAATT7910.13493895334455833No Hit
GTCCTACAGTGTGCATTTCTCATTT7320.12487397452366208No Hit
CTGTAGGACGTGGAATATGGCAAGA6330.1079852812479209No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG6030.1028674954067872No Hit
CACACACACACACACACACACACAC5960.10167334537718935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT453.418308E-516.9538691
CGCCTTG704.254598E-714.9795832
GCAGGAC400.00515878814.2974663
TAGGACC2000.014.2827684
GACGTGG1800.013.7502437
GTAGGAC4000.013.5825923
CTTAGAC500.001460524613.3443013
AGGACGT3100.013.2054595
TGTAGGA4550.013.1987922
ACGTGGA2000.012.8390928
GGACGTG3200.012.7916936
GTGTTAG751.4177347E-512.7154011
CTGTAGG4750.012.64847851
GTCCTAC4950.012.5227441
CCTGGAC1103.623427E-812.1311843
TAGGACG3300.012.1187114
AATCCCG550.00310825712.06805419
TGAGTTA2050.012.02600612
CGTGGAA2150.011.9117469
CTAGGCT657.8573835E-411.7191944
Produced by FastQC (version 0.11.5)