##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926955_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 586191 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.0248212613295 30.0 26.0 31.0 7.0 31.0 2 26.681530422677934 31.0 27.0 31.0 10.0 33.0 3 26.315700855182016 31.0 26.0 31.0 7.0 34.0 4 29.985143067703188 35.0 30.0 37.0 10.0 37.0 5 29.524794819435986 35.0 28.0 37.0 7.0 37.0 6 29.340017161641853 35.0 29.0 37.0 2.0 37.0 7 29.340527234297355 35.0 29.0 37.0 2.0 37.0 8 29.084839241817086 35.0 28.0 37.0 2.0 37.0 9 31.256605782074445 37.0 31.0 39.0 2.0 39.0 10 31.482849446682053 37.0 32.0 39.0 2.0 39.0 11 31.6820490249765 37.0 32.0 39.0 2.0 39.0 12 31.549767908412104 37.0 32.0 39.0 2.0 39.0 13 31.66212548469697 37.0 32.0 39.0 2.0 39.0 14 32.55017562535078 38.0 32.0 40.0 2.0 41.0 15 32.1247170290912 38.0 31.0 40.0 2.0 41.0 16 32.186553529481 38.0 31.0 40.0 2.0 41.0 17 32.406382902501065 38.0 32.0 40.0 2.0 41.0 18 32.442637297399656 38.0 32.0 40.0 2.0 41.0 19 31.82409999471162 38.0 31.0 40.0 2.0 41.0 20 32.11560225250814 38.0 32.0 40.0 2.0 41.0 21 32.071979951926934 38.0 32.0 40.0 2.0 41.0 22 32.06898775313848 38.0 32.0 40.0 2.0 41.0 23 32.04057380614851 38.0 32.0 40.0 2.0 41.0 24 30.765033922390483 38.0 29.0 40.0 2.0 41.0 25 31.285669687866243 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.627044651384491 1101 2 -0.8133815824522621 1101 3 -1.2924021657577853 1101 4 -1.1083988510921436 1101 5 -0.9167633289558061 1101 6 -3.2289886419556737 1101 7 -1.2911065222049913 1101 8 -0.8146599389610323 1101 9 -0.8912699835719415 1101 10 -9.664045220506086 1101 11 -4.653121748585825 1101 12 -2.21654339279306 1101 13 -1.443059883285386 1101 14 -1.583958792117759 1101 15 -2.6240868837857434 1101 16 -1.220162079733889 1101 17 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0.26766952065230143 1103 4 -0.0035002797362011506 1103 5 -0.29088758539995396 1103 6 0.7923164574393127 1103 7 0.481271331270019 1103 8 0.5662745264413758 1103 9 0.5361843862485891 1103 10 0.43110704956356827 1103 11 0.2946437707593361 1103 12 0.16039493655769732 1103 13 0.4594135520697442 1103 14 0.43259771812678594 1103 15 0.7417171733600227 1103 16 0.8302166888867859 1103 17 0.2516640602876947 1103 18 -0.005544509914486184 1103 19 -1.3695570564818595 1103 20 -0.21222568287534926 1103 21 0.1603834680723466 1103 22 0.3830676757873377 1103 23 0.1085875954600013 1103 24 1.7062430220829725 1103 25 1.237149715448254 1104 1 -0.9430381114690718 1104 2 -0.6439584927758375 1104 3 -2.057078715416761 1104 4 -1.1442727358121054 1104 5 -1.2121244271718652 1104 6 -1.891355305731583 1104 7 -0.6364186475754785 1104 8 -0.31858796928338506 1104 9 -0.30083346207235095 1104 10 0.09846070979943278 1104 11 0.021912812979675067 1104 12 -0.251788537594539 1104 13 -0.3121684754691145 1104 14 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0.906593831278439 2215 4 1.3356959618310462 2215 5 1.6809982267478354 2215 6 2.090210439086867 2215 7 1.4911140741853508 2215 8 0.17036506208747326 2215 9 0.546215745093285 2215 10 0.25453361085217097 2215 11 0.36944676547114597 2215 12 -0.2995617859223145 2215 13 0.32513752480722147 2215 14 0.5820162487739822 2215 15 0.7818877377035065 2215 16 0.8752426669703084 2215 17 0.35617579960428003 2215 18 -0.14790440762216406 2215 19 0.7535084730891803 2215 20 -2.9390288164455143 2215 21 -1.5798380110005397 2215 22 -0.7648153129072774 2215 23 -0.3840841549652545 2215 24 -0.3351923104249295 2215 25 -0.046403669802675296 2216 1 0.7710023381952844 2216 2 -0.37736954173308845 2216 3 0.41950655751244526 2216 4 0.14177906688286157 2216 5 0.45229024358904724 2216 6 -1.1013216755533186 2216 7 -8.654066404105926 2216 8 -9.851534641429286 2216 9 -5.042569986937959 2216 10 -2.6401212636103217 2216 11 -1.8227737506439041 2216 12 -1.4811410817828197 2216 13 -1.0393267313963293 2216 14 -1.3857810087833826 2216 15 -2.1901088782943283 2216 16 -1.331264642233588 2216 17 -1.3798085543106922 2216 18 -1.1042155388222916 2216 19 -0.6951585562012603 2216 20 -1.790544229962478 2216 21 -4.3747775487756115 2216 22 -2.5791515745917692 2216 23 -2.572151279034024 2216 24 -2.574426597852792 2216 25 -1.9842308383494043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54191.0 3 6.0 4 416.0 5 2087.0 6 2396.0 7 1989.0 8 1626.0 9 1584.0 10 1482.0 11 1424.0 12 1432.0 13 1346.0 14 1376.0 15 1449.0 16 1532.0 17 1593.0 18 1795.0 19 2383.0 20 3113.0 21 3765.0 22 4784.0 23 5604.0 24 6613.0 25 7387.0 26 8204.0 27 8973.0 28 10136.0 29 11742.0 30 13481.0 31 16416.0 32 20979.0 33 27326.0 34 36105.0 35 49701.0 36 69757.0 37 103139.0 38 98859.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2362384301604 18.485415249108435 12.699042272013896 25.579304048717272 2 13.713814255479429 20.00095658308522 43.0428224667203 23.242406694715047 3 20.16979987871437 23.996788493239606 28.702841671008468 27.130569957037554 4 10.939812593997477 16.669711004170455 39.42491041965766 32.96556598217441 5 11.959615332113898 38.1427625651806 36.27914357476317 13.61847852794234 6 30.51983728850577 37.76148842796399 19.69140099783643 12.02727328569381 7 27.607330478828136 30.908372435948973 23.21711745154155 18.267179633681337 8 24.86854394241592 37.942477497665095 20.83865030464963 16.35032825526936 9 25.917268396554782 16.091269570439934 20.430379055704943 37.56108297730034 10 13.236368228484055 30.650836882918302 35.75746095456531 20.35533393403233 11 34.19171780972904 23.434698115171276 24.02469881470941 18.348885260390276 12 21.992301602467855 25.945920122300787 32.70010373727171 19.361674537959654 13 28.38911116608799 22.646283515752298 25.76671415531347 23.197891162846247 14 19.539357167962816 23.13639109084891 28.90184850894225 28.422403232246022 15 22.743760535600945 31.857268626260005 23.456769059627234 21.942201778511812 16 20.701098826098825 29.986691236691236 28.792656292656293 20.519553644553646 17 20.61182147329914 29.653668574236548 28.471248246844322 21.263261705619993 18 19.958130512005486 29.493420143420913 30.23048692474241 20.317962419831193 19 22.603587047587848 28.05138784376157 28.216389985206114 21.128635123444468 20 21.935655592257923 28.691033550817615 29.3462163195108 20.027094537413667 21 22.10197472812085 27.897342801472767 27.932831262604374 22.067851207802004 22 21.166777286198606 29.377419624530575 28.711143112099958 20.74465997717086 23 21.12457131157331 28.85290655018854 28.540326571004453 21.4821955672337 24 21.938518709360398 28.290538938093867 29.23645744646364 20.5344849060821 25 21.472067101062787 28.521022827123655 29.016159330900305 20.990750740913256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 252.5 3 320.0 4 320.0 5 320.0 6 479.0 7 638.0 8 638.0 9 638.0 10 1014.5 11 1391.0 12 1391.0 13 1391.0 14 2203.0 15 3015.0 16 3015.0 17 3015.0 18 5196.0 19 7377.0 20 7377.0 21 7377.0 22 11755.5 23 16134.0 24 16134.0 25 16134.0 26 22789.5 27 29445.0 28 29445.0 29 29445.0 30 38372.5 31 47300.0 32 47300.0 33 47300.0 34 54779.0 35 62258.0 36 62258.0 37 62258.0 38 67922.0 39 73586.0 40 73586.0 41 73586.0 42 76466.5 43 79347.0 44 79347.0 45 79347.0 46 80082.5 47 80818.0 48 80818.0 49 80818.0 50 75953.0 51 71088.0 52 71088.0 53 71088.0 54 60416.0 55 49744.0 56 49744.0 57 49744.0 58 40860.0 59 31976.0 60 31976.0 61 31976.0 62 24810.5 63 17645.0 64 17645.0 65 17645.0 66 12940.0 67 8235.0 68 8235.0 69 8235.0 70 5855.5 71 3476.0 72 3476.0 73 3476.0 74 2440.5 75 1405.0 76 1405.0 77 1405.0 78 964.0 79 523.0 80 523.0 81 523.0 82 343.5 83 164.0 84 164.0 85 164.0 86 120.0 87 76.0 88 76.0 89 76.0 90 51.5 91 27.0 92 27.0 93 27.0 94 19.5 95 12.0 96 12.0 97 12.0 98 9.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21579996963447065 2 0.13203887470124925 3 0.13579191765141396 4 0.06891951599393371 5 0.021665293394132628 6 0.020982921948648137 7 0.27601924969847713 8 0.27038968527323004 9 0.017571064721225674 10 0.03531272230382247 11 0.015353357523401076 12 0.016206321830256692 13 0.014670986077916582 14 0.016547507552998938 15 0.014159207493803214 16 0.01893580761219466 17 0.017912250443967923 18 0.014329800355174338 19 0.023541814869214982 20 0.016206321830256692 21 0.01450039321654546 22 0.01671810041437006 23 0.01722987899848343 24 0.016035728968885568 25 0.015353357523401076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 586191.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.70902620752732 #Duplication Level Percentage of deduplicated Percentage of total 1 96.08989481284166 87.16220786856597 2 3.0529088893799665 5.53852784911921 3 0.3777815072166286 1.02804577916497 4 0.15176035485189063 0.5506413602209517 5 0.07952146927325963 0.3606657520184593 6 0.04973447771222133 0.27068196253293403 7 0.03809117658159273 0.24186494743706718 8 0.02364712624399585 0.1716006235359461 9 0.017629788372782434 0.14392628419858938 >10 0.10115451614522272 1.8341749030584595 >50 0.00903206145606478 0.553019564776228 >100 0.007526655255275864 1.3338946174944588 >500 0.0013171646695430412 0.8107484878767739 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 879 0.1499511251452172 No Hit GTCCTACAGTGGACATTTCTAAATT 791 0.13493895334455833 No Hit GTCCTACAGTGTGCATTTCTCATTT 732 0.12487397452366208 No Hit CTGTAGGACGTGGAATATGGCAAGA 633 0.1079852812479209 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 603 0.1028674954067872 No Hit CACACACACACACACACACACACAC 596 0.10167334537718935 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.7059286137112307E-4 0.0 11 0.0 0.0 0.0 1.7059286137112307E-4 0.0 12 0.0 0.0 0.0 1.7059286137112307E-4 0.0 13 0.0 0.0 0.0 3.4118572274224613E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTT 45 3.418308E-5 16.953869 1 CGCCTTG 70 4.254598E-7 14.979583 2 GCAGGAC 40 0.005158788 14.297466 3 TAGGACC 200 0.0 14.282768 4 GACGTGG 180 0.0 13.750243 7 GTAGGAC 400 0.0 13.582592 3 CTTAGAC 50 0.0014605246 13.344301 3 AGGACGT 310 0.0 13.205459 5 TGTAGGA 455 0.0 13.198792 2 ACGTGGA 200 0.0 12.839092 8 GGACGTG 320 0.0 12.791693 6 GTGTTAG 75 1.4177347E-5 12.715401 1 CTGTAGG 475 0.0 12.6484785 1 GTCCTAC 495 0.0 12.522744 1 CCTGGAC 110 3.623427E-8 12.131184 3 TAGGACG 330 0.0 12.118711 4 AATCCCG 55 0.003108257 12.068054 19 TGAGTTA 205 0.0 12.026006 12 CGTGGAA 215 0.0 11.911746 9 CTAGGCT 65 7.8573835E-4 11.719194 4 >>END_MODULE