FastQCFastQC Report
Thu 26 May 2016
SRR1926955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586191
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14290.24377719889933486No Hit
GGTATCAACGCAGAGTACTTTTTTT9900.16888693275741185No Hit
CACACACACACACACACACACACAC8920.15216883234304177No Hit
GTCCTACAGTGGACATTTCTAAATT8460.14432156071997013No Hit
CTGTAGGACGTGGAATATGGCAAGA8080.13783903198786743No Hit
GTCCTACAGTGTGCATTTCTCATTT7860.13408598903770275No Hit
TATCAACGCAGAGTACTTTTTTTTT7330.1250445673850332No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG7290.12436219593954873No Hit
CTGTAGGACCTGGAATATGGCGAGA5930.10116156679307597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2200.014.2864184
AAAGGCG500.001469062513.3339895
TCCAACG500.001512277513.28280218
GACAGAT655.494433E-513.14027
TGTAGGA5550.013.0534332
CTGTAGG5000.012.9630111
GACGTGG2250.012.6535257
GTCCTAC4300.012.6349451
TATGGCG3700.012.56481216
TGGCGAG3650.012.47699618
AGGACGT4050.012.463875
AGGACCT3450.012.4229725
GTAGGAC5000.012.3815623
CCTACAC1001.3894532E-712.3815613
GGACGTG3950.012.2971696
ACGTGGA2400.012.2549668
GTCTAAG701.05539184E-412.2549461
TAGACAG701.0630196E-412.2455015
TAGGACG4050.012.2287034
TATTAGG550.002987237412.1321992
Produced by FastQC (version 0.11.5)