##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926955_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 586191 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.732495040012555 31.0 30.0 33.0 25.0 34.0 2 30.06469393081777 31.0 30.0 34.0 26.0 34.0 3 30.126736848569834 31.0 30.0 34.0 26.0 34.0 4 33.718103826227285 35.0 35.0 37.0 28.0 37.0 5 33.656543686272904 37.0 35.0 37.0 28.0 37.0 6 33.567567567567565 37.0 35.0 37.0 28.0 37.0 7 33.54901559389346 37.0 35.0 37.0 28.0 37.0 8 33.57315960156331 37.0 35.0 37.0 28.0 37.0 9 35.099411284035405 39.0 35.0 39.0 28.0 39.0 10 35.01630014790401 39.0 35.0 39.0 28.0 39.0 11 35.01810843223454 39.0 35.0 39.0 28.0 39.0 12 34.91796871668108 39.0 35.0 39.0 27.0 39.0 13 34.92421241540727 39.0 35.0 39.0 27.0 39.0 14 36.00480730683344 40.0 36.0 41.0 27.0 41.0 15 35.982239577202655 40.0 36.0 41.0 27.0 41.0 16 35.95853740504375 40.0 36.0 41.0 27.0 41.0 17 35.94107040196796 40.0 36.0 41.0 27.0 41.0 18 35.918750031986164 40.0 36.0 41.0 27.0 41.0 19 36.008635410642604 40.0 36.0 41.0 27.0 41.0 20 35.9575854968773 40.0 36.0 41.0 27.0 41.0 21 35.910706578572515 40.0 36.0 41.0 27.0 41.0 22 35.82902330469079 40.0 36.0 41.0 27.0 41.0 23 35.74960720993669 40.0 36.0 41.0 27.0 41.0 24 35.674660648150514 40.0 36.0 41.0 27.0 41.0 25 35.56636829975213 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25639570150717006 1101 2 -0.19359403687018428 1101 3 0.059051545261063154 1101 4 -0.21817098317607986 1101 5 -0.3125483305971173 1101 6 -0.27179432185871377 1101 7 -0.24535071139167997 1101 8 -0.20072171664799754 1101 9 -0.36302271274314535 1101 10 -0.348552437859766 1101 11 -0.1522701263975179 1101 12 -0.11665405676545504 1101 13 -0.2987914709658739 1101 14 -0.2906827716699425 1101 15 -0.032676856866181936 1101 16 -0.20867998859171166 1101 17 -0.3250366089647514 1101 18 -0.23898035429120057 1101 19 -0.2910461336242278 1101 20 -0.3981200398533744 1101 21 -0.057798809992846145 1101 22 -0.6148470669261314 1101 23 -0.584382264199867 1101 24 -0.24859778202218052 1101 25 -0.5212638149143913 1102 1 -0.6619444923769073 1102 2 -0.6950219301922367 1102 3 -0.32610300774512524 1102 4 -0.5779562445308599 1102 5 -0.46250679657275384 1102 6 -0.4154801182542798 1102 7 -0.5438578082923655 1102 8 -0.5476907647707066 1102 9 -0.6795919507356842 1102 10 -0.6725410456702292 1102 11 -0.6172461088964738 1102 12 -0.659636612475218 1102 13 -0.6882168051728854 1102 14 -0.6121470911052853 1102 15 -0.5162967147733823 1102 16 -0.6723506160392532 1102 17 -0.6620736077832277 1102 18 -0.5574121739974203 1102 19 -0.5315887588841335 1102 20 -0.5119297159911795 1102 21 -0.5503213957309399 1102 22 -0.3360144567835732 1102 23 -0.43598970833531325 1102 24 -0.3122970447287585 1102 25 -0.3760727326047899 1103 1 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25 0.5599053526939315 2207 1 0.18633886692107282 2207 2 0.220198330584779 2207 3 0.46974906084204093 2207 4 0.48169241646535 2207 5 0.5686520450538666 2207 6 0.3210725781668984 2207 7 0.4301594055723754 2207 8 0.33803863509793075 2207 9 0.379442923729151 2207 10 0.4274656616877408 2207 11 0.3448484597887713 2207 12 0.6190882808081852 2207 13 0.7057974473300348 2207 14 0.5180553825177086 2207 15 0.48266933966758074 2207 16 0.6381355155489885 2207 17 0.6763720822330583 2207 18 0.6383781362748309 2207 19 0.6087873846887604 2207 20 0.504018609510581 2207 21 0.45528494310200784 2207 22 0.5663618462472613 2207 23 0.6178716417166399 2207 24 0.4754694936544155 2207 25 0.39348902381181006 2208 1 0.37763022487169806 2208 2 0.1344125670187708 2208 3 0.34862718424846406 2208 4 0.09716471451256581 2208 5 0.40900480625013813 2208 6 0.015079837368389803 2208 7 0.0033627532041364816 2208 8 -0.0611130582158097 2208 9 0.02568463714067093 2208 10 0.2693098588260554 2208 11 -0.037035932928034754 2208 12 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2211 10 -0.16384906933286913 2211 11 -0.3971167830088831 2211 12 -0.1723353036231714 2211 13 -0.43793174245908517 2211 14 -0.35126597931197523 2211 15 -0.47605292524225007 2211 16 -0.3965256396726531 2211 17 -0.45305320021663675 2211 18 -0.3225022245777822 2211 19 -0.49454035182432676 2211 20 -0.3138440217538303 2211 21 -0.37831163050566374 2211 22 -0.4016117728084865 2211 23 -0.4545431490666303 2211 24 -0.6100231213887 2211 25 -0.8540315373879963 2212 1 0.4516531483187052 2212 2 0.45628040344158194 2212 3 0.4775135575327454 2212 4 0.5648879351153653 2212 5 0.6903632327394504 2212 6 0.6767297595418711 2212 7 0.6306614655290232 2212 8 0.5809868427322584 2212 9 0.7150351394754679 2212 10 0.8469297824145698 2212 11 0.8989878395355291 2212 12 0.8468305799406224 2212 13 0.6526441737712787 2212 14 0.6124763574670098 2212 15 0.45880522126510925 2212 16 0.7720797659746381 2212 17 0.7515950747195959 2212 18 0.8887375391488916 2212 19 0.5726588247989355 2212 20 0.7092228328575274 2212 21 0.5092773076068369 2212 22 0.5772142441065355 2212 23 0.6380621223344463 2212 24 0.6280457469157739 2212 25 0.5076646634443307 2213 1 0.2168336388119556 2213 2 0.16650360542559994 2213 3 0.2701065583594193 2213 4 0.3653012721228066 2213 5 0.10698489159864266 2213 6 0.3863742897154694 2213 7 0.3548279025640255 2213 8 0.24503203424507802 2213 9 0.2973773825176309 2213 10 0.34424584744625975 2213 11 0.4344576659339907 2213 12 0.31310779129361066 2213 13 0.3052261522777542 2213 14 0.24946726405284636 2213 15 0.2770802281540554 2213 16 0.3033776441779992 2213 17 0.3353206189466533 2213 18 0.41823326799981686 2213 19 0.28836563108165336 2213 20 0.4192531442380769 2213 21 0.3389218377804326 2213 22 0.3014853931834196 2213 23 0.5558797029351652 2213 24 0.24870157496757628 2213 25 0.49964592549999054 2214 1 0.3456119871217531 2214 2 0.24314031916115653 2214 3 0.338095432985007 2214 4 0.4469230093928118 2214 5 0.22147569176980397 2214 6 0.37516333550066605 2214 7 0.2662742012375361 2214 8 0.19492078050095785 2214 9 0.11289514032678483 2214 10 -0.1396672890535271 2214 11 -0.15410031438266003 2214 12 0.07143684563678221 2214 13 0.042759314451487285 2214 14 0.0898951630686824 2214 15 -0.15470625023704088 2214 16 0.04580811058792733 2214 17 0.03256280978961712 2214 18 0.08759783796130449 2214 19 0.14791847661010848 2214 20 0.18435797949695143 2214 21 0.11746698112660425 2214 22 0.016305675596768765 2214 23 0.021014491125150414 2214 24 -0.08172418455317398 2214 25 -0.07284016778199742 2215 1 0.6154547022842287 2215 2 0.5029554456337415 2215 3 0.560435573482188 2215 4 0.5602982909314136 2215 5 0.3198896560845199 2215 6 0.3608498710477974 2215 7 0.33914141549155374 2215 8 0.41960951811954317 2215 9 0.17999866280382548 2215 10 0.2164036879796214 2215 11 0.3242934293900035 2215 12 0.1777443569611279 2215 13 0.0363216046057957 2215 14 0.23240184045265266 2215 15 0.10292878363132019 2215 16 0.12332018848934467 2215 17 0.3866870886740088 2215 18 0.22358098332313858 2215 19 -0.008544770566132343 2215 20 0.18163050886488463 2215 21 0.2755807352816575 2215 22 0.10699355080713957 2215 23 0.22903488749801681 2215 24 0.12012224817479478 2215 25 0.26644629408365716 2216 1 -0.04613632595117423 2216 2 0.0477721327481575 2216 3 -1.9610652073992938 2216 4 -1.3225727700495753 2216 5 -0.42864461788175134 2216 6 -0.10408378959544962 2216 7 -0.24002639577481233 2216 8 -0.20183971473417728 2216 9 -0.06516638391226337 2216 10 -0.20465286053517673 2216 11 -0.11998162037956206 2216 12 0.12004777675140588 2216 13 -0.0888350368267794 2216 14 -0.13056741350182932 2216 15 -0.15265969018589232 2216 16 -0.19130774091065206 2216 17 -0.1772997736927593 2216 18 -0.3171512613362779 2216 19 -0.3538235293859344 2216 20 -0.03585620192222905 2216 21 -0.0379549450373986 2216 22 0.023294219180399978 2216 23 -0.13303802176979218 2216 24 -0.21515881990909236 2216 25 -0.07993346932074985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15028.0 3 15.0 4 246.0 5 1759.0 6 2434.0 7 1844.0 8 1345.0 9 1196.0 10 1030.0 11 928.0 12 847.0 13 856.0 14 802.0 15 809.0 16 803.0 17 883.0 18 949.0 19 1076.0 20 1449.0 21 1902.0 22 2354.0 23 3020.0 24 3429.0 25 4247.0 26 4909.0 27 5405.0 28 6079.0 29 7379.0 30 9629.0 31 12419.0 32 16032.0 33 20564.0 34 28136.0 35 39067.0 36 57248.0 37 99307.0 38 230766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94324613266934 19.515290002834963 10.367417760517535 28.174046103978167 2 13.631480883751829 20.986288081783076 41.323441761492205 24.058789272972884 3 18.79938507003274 24.438688718150843 29.0095430169447 27.752383194871715 4 11.19328000600487 17.642543130140176 38.862247970371435 32.30192889348352 5 12.143566366518014 38.8940449096995 35.98218215690757 12.980206566874916 6 30.898575431281632 38.818684563207 18.11956244533562 12.163177560175747 7 28.240408962782475 31.846645781718163 21.223987660135002 18.68895759536436 8 24.28957982161015 39.38157275153037 19.637385435362685 16.691461991496794 9 25.701675217058245 16.176533312405205 20.019176535018502 38.10261493551805 10 13.225456995897058 31.308249383705956 34.92071345099077 20.545580169406225 11 34.13760336288711 23.520931890958657 23.834837749909155 18.50662699624507 12 22.117174212324738 26.20699537860785 32.2226524982642 19.453177910803205 13 28.29445462330812 22.530033334356904 26.02860545018441 23.14690659215056 14 19.48153417572088 23.367298372032323 28.600575580314263 28.55059187193253 15 22.216630357456637 31.922022488394987 23.55395798822524 22.307389165923137 16 20.770675569400208 30.226361212377622 28.426253055765482 20.576710162456692 17 20.425253884825935 29.878657297706717 28.336668423773137 21.359420393694208 18 19.89859891126893 29.50986630546225 30.325135153116666 20.26639963015215 19 21.909107968406147 28.253467305822344 28.60437742029035 21.233047305481158 20 21.958330418998845 28.7724140754679 29.334525225057707 19.934730280475552 21 21.751533402148045 27.877123090178547 28.3138974433345 22.057446064338908 22 21.161532674503704 29.468722651831914 28.598187280255072 20.771557393409314 23 20.950509304987623 28.834970171838187 28.55127424337801 21.663246279796176 24 21.062831271142926 28.42717962977811 29.753373058859857 20.75661604021911 25 21.278577606722997 28.56523823415511 28.947893688999894 21.208290470121995 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 229.0 2 231.0 3 233.0 4 233.0 5 233.0 6 385.5 7 538.0 8 538.0 9 538.0 10 897.5 11 1257.0 12 1257.0 13 1257.0 14 2047.5 15 2838.0 16 2838.0 17 2838.0 18 4992.0 19 7146.0 20 7146.0 21 7146.0 22 11358.0 23 15570.0 24 15570.0 25 15570.0 26 22301.0 27 29032.0 28 29032.0 29 29032.0 30 37990.0 31 46948.0 32 46948.0 33 46948.0 34 54415.5 35 61883.0 36 61883.0 37 61883.0 38 67751.5 39 73620.0 40 73620.0 41 73620.0 42 76685.0 43 79750.0 44 79750.0 45 79750.0 46 81176.0 47 82602.0 48 82602.0 49 82602.0 50 77504.5 51 72407.0 52 72407.0 53 72407.0 54 61268.5 55 50130.0 56 50130.0 57 50130.0 58 40963.0 59 31796.0 60 31796.0 61 31796.0 62 24553.0 63 17310.0 64 17310.0 65 17310.0 66 12775.0 67 8240.0 68 8240.0 69 8240.0 70 5689.5 71 3139.0 72 3139.0 73 3139.0 74 2088.5 75 1038.0 76 1038.0 77 1038.0 78 694.0 79 350.0 80 350.0 81 350.0 82 221.0 83 92.0 84 92.0 85 92.0 86 63.5 87 35.0 88 35.0 89 35.0 90 21.5 91 8.0 92 8.0 93 8.0 94 4.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11003239558437439 2 0.10952061700026101 3 0.018424029028081292 4 0.0 5 0.006311935870731554 6 0.3316325225054632 7 0.020982921948648137 8 0.010064978820896261 9 0.009894385959525137 10 0.0044354143956492 11 0.004776600118391446 12 6.823714454844923E-4 13 0.0015353357523401076 14 0.0 15 0.003753042950164707 16 6.823714454844923E-4 17 0.0 18 6.823714454844923E-4 19 1.7059286137112307E-4 20 0.0013647428909689845 21 0.012965057464205353 22 0.0 23 0.0 24 3.4118572274224613E-4 25 0.004094228672906953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 586191.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.96504923151535 #Duplication Level Percentage of deduplicated Percentage of total 1 94.63878698126288 83.24905556017683 2 4.267717232432957 7.50819912914303 3 0.5068763922227202 1.3376222038849341 4 0.17635088164206528 0.6205085594266163 5 0.09121665039240921 0.40119385712510997 6 0.07042370594025876 0.3716894856060373 7 0.0453715459138082 0.27937771890126684 8 0.028329469654999138 0.19936025543237754 9 0.02524960727614542 0.1998974652410304 >10 0.1285134345589863 2.2639300434874907 >50 0.010290823601986736 0.6553537318060877 >100 0.008737453206060028 1.4082285964815646 >500 0.0019416562679263085 1.2615132096224704 >1k 1.9416562679263087E-4 0.24407018366511105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1429 0.24377719889933486 No Hit GGTATCAACGCAGAGTACTTTTTTT 990 0.16888693275741185 No Hit CACACACACACACACACACACACAC 892 0.15216883234304177 No Hit GTCCTACAGTGGACATTTCTAAATT 846 0.14432156071997013 No Hit CTGTAGGACGTGGAATATGGCAAGA 808 0.13783903198786743 No Hit GTCCTACAGTGTGCATTTCTCATTT 786 0.13408598903770275 No Hit TATCAACGCAGAGTACTTTTTTTTT 733 0.1250445673850332 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 729 0.12436219593954873 No Hit CTGTAGGACCTGGAATATGGCGAGA 593 0.10116156679307597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 220 0.0 14.286418 4 AAAGGCG 50 0.0014690625 13.333989 5 TCCAACG 50 0.0015122775 13.282802 18 GACAGAT 65 5.494433E-5 13.1402 7 TGTAGGA 555 0.0 13.053433 2 CTGTAGG 500 0.0 12.963011 1 GACGTGG 225 0.0 12.653525 7 GTCCTAC 430 0.0 12.634945 1 TATGGCG 370 0.0 12.564812 16 TGGCGAG 365 0.0 12.476996 18 AGGACGT 405 0.0 12.46387 5 AGGACCT 345 0.0 12.422972 5 GTAGGAC 500 0.0 12.381562 3 CCTACAC 100 1.3894532E-7 12.381561 3 GGACGTG 395 0.0 12.297169 6 ACGTGGA 240 0.0 12.254966 8 GTCTAAG 70 1.05539184E-4 12.254946 1 TAGACAG 70 1.0630196E-4 12.245501 5 TAGGACG 405 0.0 12.228703 4 TATTAGG 55 0.0029872374 12.132199 2 >>END_MODULE