FastQCFastQC Report
Thu 26 May 2016
SRR1926954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587914
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11640.19798814112268118No Hit
GTCCTACAGTGGACATTTCTAAATT8790.14951166327047835No Hit
GTACATGGGAAGCAGTGGTATCAAC8570.14576961936609775No Hit
CCCATGTACTCTGCGTTGATACCAC7270.12365754174930346No Hit
GTCCTACAGTGTGCATTTCTCATTT7060.12008559074966746No Hit
CTGTAGGACGTGGAATATGGCAAGA7030.11957531203543376No Hit
GGTATCAACGCAGAGTACTTTTTTT6420.10919964484601491No Hit
GTCCTAAAGTGTGTATTTCTCATTT5970.10154546413250919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA551.1454133E-515.51733319
TCCAACG755.9550075E-815.17250318
CGCCTTG456.577635E-414.8292662
ATAGGAC602.4801626E-514.2996493
GGACGTG2700.013.3940446
TAGGACA1900.013.0252414
TAGGACG3150.012.9934344
AGGACGT3050.012.7931035
TGTAGGA5000.012.0117052
GTCCTAA2500.011.8331881
GTAGGAC4600.011.8127543
ATAAGCC657.7799254E-411.7330463
CAATACT657.881024E-411.7150114
TTAGGAC1800.011.65156653
GACGTGG1800.011.6316697
TGGCGAG3400.011.43515918
TAAGACA752.0326549E-411.4221354
CAGGATA1001.8782921E-611.4221344
CTGTAGG4950.011.3743581
TATGGCG3600.011.326694516
Produced by FastQC (version 0.11.5)