##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926954_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 587914 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.246651721170103 30.0 26.0 31.0 10.0 31.0 2 26.874032596604266 31.0 27.0 31.0 10.0 34.0 3 26.503798174562945 31.0 26.0 31.0 10.0 34.0 4 30.18421741955456 35.0 30.0 37.0 10.0 37.0 5 29.763904244498345 35.0 30.0 37.0 10.0 37.0 6 29.53733879444953 35.0 29.0 37.0 8.0 37.0 7 29.555239371744847 35.0 29.0 37.0 2.0 37.0 8 29.28632759213082 35.0 29.0 37.0 2.0 37.0 9 31.43248332239069 37.0 31.0 39.0 2.0 39.0 10 31.630835462329525 37.0 32.0 39.0 2.0 39.0 11 31.830061879798745 37.0 32.0 39.0 2.0 39.0 12 31.701381494572335 37.0 32.0 39.0 2.0 39.0 13 31.808305296352867 37.0 32.0 39.0 2.0 39.0 14 32.71486986191858 38.0 32.0 40.0 2.0 41.0 15 32.28409597322057 38.0 32.0 40.0 2.0 41.0 16 32.35888582343676 38.0 32.0 40.0 2.0 41.0 17 32.583597260823865 38.0 32.0 40.0 2.0 41.0 18 32.62934374755491 38.0 32.0 40.0 2.0 41.0 19 32.016672506523065 38.0 31.0 40.0 2.0 41.0 20 32.28336287280112 38.0 32.0 40.0 2.0 41.0 21 32.24513619338883 38.0 32.0 40.0 2.0 41.0 22 32.269857496164406 39.0 32.0 40.0 2.0 41.0 23 32.25483829267546 39.0 32.0 40.0 2.0 41.0 24 30.97909728293594 38.0 30.0 40.0 2.0 41.0 25 31.50431525699337 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.240350295920667 1101 2 -1.1231945336686557 1101 3 -1.9414644742003553 1101 4 -1.6893538589609456 1101 5 -1.503597082229419 1101 6 -3.4976634066433405 1101 7 -1.9826805033417472 1101 8 -1.0909125629664231 1101 9 -1.5013611732311922 1101 10 -9.495317748535193 1101 11 -4.880961480430273 1101 12 -2.676427615279181 1101 13 -1.8378972787081942 1101 14 -1.7236155809782154 1101 15 -3.3948146841401403 1101 16 -1.5493815896699452 1101 17 -1.2015463968675135 1101 18 -1.4256783472872065 1101 19 -2.1631396380633063 1101 20 -1.4106119564766217 1101 21 -1.4989365467189053 1101 22 -1.1750699998542267 1101 23 -1.3116108332635363 1101 24 -1.4944886719001715 1101 25 -1.3577434077853283 1102 1 -0.8646472588003071 1102 2 -0.541129748514205 1102 3 -0.9880429944458804 1102 4 -0.8407674582125964 1102 5 -0.9585527560227902 1102 6 -1.3945474034101792 1102 7 -0.4095185845677527 1102 8 0.08291648694904907 1102 9 -0.2342388541785141 1102 10 -0.1266428121409966 1102 11 -0.2997633689485326 1102 12 -0.245280816587492 1102 13 -0.4571524842642454 1102 14 -0.8011167064863152 1102 15 -0.0472850284612889 1102 16 -0.08283999387461449 1102 17 -0.21724642398349658 1102 18 -0.5965700481083402 1102 19 0.02355199088100335 1102 20 -0.18431022689178178 1102 21 -0.18631256243320138 1102 22 -0.32415603538006366 1102 23 -0.5657719696958736 1102 24 -3.635567057863174 1102 25 -2.1623993136863646 1103 1 -1.1210520503066306 1103 2 -0.8179030465887855 1103 3 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0.1183821424678051 1107 12 0.46123325402210114 1107 13 0.07217526424087595 1107 14 0.4136734677682199 1107 15 -1.3443798817362058 1107 16 -0.34359029482383363 1107 17 -0.1328202996174639 1107 18 -0.04307879043660279 1107 19 0.9432996868926864 1107 20 0.7642385564839813 1107 21 0.5132007385357582 1107 22 0.32910729025686436 1107 23 -0.07465490538154285 1107 24 -3.7306250769265787 1107 25 -1.7534401539760651 1108 1 -0.012474189725978135 1108 2 -0.3044165279322648 1108 3 0.3662031008983959 1108 4 0.2544088361222947 1108 5 0.45306738991029505 1108 6 -2.716197462639503 1108 7 -1.332186989955133 1108 8 0.0678142541529283 1108 9 -0.04618948977384463 1108 10 0.43116177091444285 1108 11 0.14243702524997914 1108 12 0.3078700340252034 1108 13 0.2083536526898726 1108 14 0.10511307341951692 1108 15 -0.9542327772493273 1108 16 -0.028014297307919378 1108 17 -0.09099837755567819 1108 18 -0.3197924218602992 1108 19 0.040392529618969775 1108 20 -1.1543396922351619 1108 21 -0.41455698542015185 1108 22 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1110 9 1.3331800393067077 1110 10 1.3559609503365735 1110 11 1.0774333739667448 1110 12 1.1989861256147627 1110 13 1.0548099862249884 1110 14 0.965037117119607 1110 15 1.5995347705296368 1110 16 1.4280537991243918 1110 17 0.8647009873132134 1110 18 1.1171196494742475 1110 19 -0.6818288918832458 1110 20 1.0197424424203234 1110 21 0.7910559016879333 1110 22 1.085268356603514 1110 23 1.0978620955899387 1110 24 0.18952035995552663 1110 25 0.5058282327888897 1111 1 0.6917811993073286 1111 2 0.34642009778682237 1111 3 0.8667380158281546 1111 4 0.7108144424061358 1111 5 0.5654945429428864 1111 6 0.8669818089377443 1111 7 1.0331833682501816 1111 8 1.1461734260670795 1111 9 0.2908808186937648 1111 10 0.2912991126687743 1111 11 0.06852609327802028 1111 12 0.3156673575736626 1111 13 0.06418704076596171 1111 14 0.20358551591615992 1111 15 -0.9295445763465402 1111 16 -0.37337578718676667 1111 17 -0.05408455516619881 1111 18 0.19679697931651674 1111 19 0.7491149275393667 1111 20 0.01731873173653753 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1.4105076860380983 2215 5 1.7372877689723119 2215 6 2.0278281903288615 2215 7 1.704035287999794 2215 8 0.5168173353753858 2215 9 0.7679653237405439 2215 10 0.7578564432658439 2215 11 0.47001844476200105 2215 12 0.2588884497036119 2215 13 0.4674181792206298 2215 14 0.68484418301788 2215 15 0.44197301974827496 2215 16 0.8197025526795372 2215 17 0.5441365154972431 2215 18 -0.3245396541343126 2215 19 0.7309177705634013 2215 20 -3.153524601410755 2215 21 -1.521127871450748 2215 22 -0.5181430471250543 2215 23 -0.23946597150840176 2215 24 -0.516556251015647 2215 25 -0.49728293400305645 2216 1 0.014452535221902707 2216 2 -0.7427559608089496 2216 3 0.09411723603087196 2216 4 -0.4545903764549948 2216 5 0.016874355022100218 2216 6 -1.743193534473928 2216 7 -9.08894048190642 2216 8 -10.28177794141424 2216 9 -5.56590973101153 2216 10 -3.355794527125056 2216 11 -2.543449541139598 2216 12 -2.1242941704541565 2216 13 -1.7472263109813042 2216 14 -2.0830060153176575 2216 15 -2.6447286745980385 2216 16 -1.9226618361645471 2216 17 -2.257838038605989 2216 18 -1.7980734243925767 2216 19 -1.2759165158994321 2216 20 -2.1284061936623644 2216 21 -4.489534391757115 2216 22 -3.2347985245835744 2216 23 -3.379071470049027 2216 24 -3.3097786610892115 2216 25 -2.8320227644456146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52924.0 3 12.0 4 431.0 5 2077.0 6 2472.0 7 1998.0 8 1670.0 9 1523.0 10 1427.0 11 1385.0 12 1373.0 13 1322.0 14 1349.0 15 1386.0 16 1426.0 17 1628.0 18 1852.0 19 2314.0 20 2908.0 21 3725.0 22 4702.0 23 5515.0 24 6370.0 25 6971.0 26 7820.0 27 8790.0 28 9703.0 29 11275.0 30 13539.0 31 16288.0 32 20379.0 33 26644.0 34 35525.0 35 48942.0 36 68879.0 37 104403.0 38 106967.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93080165323075 18.884614859980083 12.835215724788911 25.349367762000245 2 13.775130432116534 20.332080253863566 42.463893767022604 23.428895546997293 3 19.67513299072855 24.836348584753804 29.04112109097537 26.447397333542284 4 11.270278688887338 16.916907375476807 39.21014602528336 32.602667910352494 5 12.0844328074572 38.158770018084965 36.14594551372024 13.610851660737588 6 30.229776722219576 37.87079695400383 19.562495321442423 12.336931002334175 7 26.804857078519813 31.57476338971948 23.676040095804137 17.944339435956575 8 24.978292576828924 37.497803669364814 21.2507569877432 16.273146766063068 9 25.887030370667773 16.15436437996339 20.76831733026655 37.19028791910229 10 13.755515359627823 30.499917471672365 35.22343055794254 20.52113661075727 11 33.445102812610045 23.832247110920576 24.350435594751428 18.37221448171795 12 22.250575862432676 26.167971501189154 32.295249857948264 19.28620277842991 13 28.16022537434802 22.98059616686684 25.983716158973298 22.875462299811847 14 19.88972645969538 23.369756605439505 28.458807337732704 28.28170959713241 15 22.71316827202793 31.780198767654156 23.64491291581465 21.86172004450327 16 20.693028375442545 29.85503498656849 28.85673115038015 20.595205487608816 17 20.66658273846384 29.793604670944735 28.38684323345877 21.15296935713265 18 20.292537536873407 29.11374531746005 30.313258095137645 20.2804590505289 19 22.707954785188814 28.109198804978803 28.156155788298864 21.02669062153352 20 22.278419423930483 28.665846675206826 29.028548291882935 20.027185608979757 21 21.925573087228344 27.930421468974608 28.119763535065704 22.02424190873134 22 21.6834777401426 29.263176518823354 28.32579401061913 20.727551730414913 23 21.07613553428251 28.89009487808073 28.500170974486522 21.533598613150236 24 22.315902649477557 28.2281469988806 28.89740632240811 20.558544029233737 25 21.580622606618704 28.357878870876917 28.75408926897565 21.30740925352872 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 289.0 1 289.0 2 386.5 3 484.0 4 484.0 5 484.0 6 679.5 7 875.0 8 875.0 9 875.0 10 1274.0 11 1673.0 12 1673.0 13 1673.0 14 2613.5 15 3554.0 16 3554.0 17 3554.0 18 5915.0 19 8276.0 20 8276.0 21 8276.0 22 12766.5 23 17257.0 24 17257.0 25 17257.0 26 24292.0 27 31327.0 28 31327.0 29 31327.0 30 40020.0 31 48713.0 32 48713.0 33 48713.0 34 55881.5 35 63050.0 36 63050.0 37 63050.0 38 67268.0 39 71486.0 40 71486.0 41 71486.0 42 73951.5 43 76417.0 44 76417.0 45 76417.0 46 77422.5 47 78428.0 48 78428.0 49 78428.0 50 73097.0 51 67766.0 52 67766.0 53 67766.0 54 58263.0 55 48760.0 56 48760.0 57 48760.0 58 40610.0 59 32460.0 60 32460.0 61 32460.0 62 25887.0 63 19314.0 64 19314.0 65 19314.0 66 14701.0 67 10088.0 68 10088.0 69 10088.0 70 7358.0 71 4628.0 72 4628.0 73 4628.0 74 3264.5 75 1901.0 76 1901.0 77 1901.0 78 1319.5 79 738.0 80 738.0 81 738.0 82 504.0 83 270.0 84 270.0 85 270.0 86 189.5 87 109.0 88 109.0 89 109.0 90 72.5 91 36.0 92 36.0 93 36.0 94 23.0 95 10.0 96 10.0 97 10.0 98 7.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24255248216575892 2 0.1406668322237606 3 0.14338831869967378 4 0.07024836965950802 5 0.022622356331028007 6 0.02143170599781601 7 0.2922196103511738 8 0.29068877420847267 9 0.01871021952190286 10 0.04031201842446344 11 0.016499011760223434 12 0.01700929047445715 13 0.01564854723650058 14 0.017519569188690864 15 0.01564854723650058 16 0.019900869855114866 17 0.01871021952190286 18 0.01564854723650058 19 0.02415319247372915 20 0.017519569188690864 21 0.015138268522266863 22 0.018199940807669148 23 0.018199940807669148 24 0.01700929047445715 25 0.01615882595073429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 587914.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.72413279015731 #Duplication Level Percentage of deduplicated Percentage of total 1 94.28256376548097 82.70856143559527 2 4.426113691158259 7.765539703750007 3 0.6469331647920958 1.7025495256373562 4 0.21901353426916223 0.7685108945227862 5 0.11807919625638334 0.517919754607501 6 0.06671695723454119 0.3511612329478883 7 0.03904492687548754 0.2397627645005075 8 0.029351555822852194 0.2059871824481281 9 0.02111332805123265 0.16669335542476377 >10 0.12796867294514067 2.075593597656812 >50 0.012035959689684296 0.7453908326270958 >100 0.009318103094186013 1.5917600480123966 >500 0.0015530171822321693 0.9623447128385855 >1k 1.9412714777902116E-4 0.19822495943091722 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1164 0.19798814112268118 No Hit GTCCTACAGTGGACATTTCTAAATT 879 0.14951166327047835 No Hit GTACATGGGAAGCAGTGGTATCAAC 857 0.14576961936609775 No Hit CCCATGTACTCTGCGTTGATACCAC 727 0.12365754174930346 No Hit GTCCTACAGTGTGCATTTCTCATTT 706 0.12008559074966746 No Hit CTGTAGGACGTGGAATATGGCAAGA 703 0.11957531203543376 No Hit GGTATCAACGCAGAGTACTTTTTTT 642 0.10919964484601491 No Hit GTCCTAAAGTGTGTATTTCTCATTT 597 0.10154546413250919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 55 1.1454133E-5 15.517333 19 TCCAACG 75 5.9550075E-8 15.172503 18 CGCCTTG 45 6.577635E-4 14.829266 2 ATAGGAC 60 2.4801626E-5 14.299649 3 GGACGTG 270 0.0 13.394044 6 TAGGACA 190 0.0 13.025241 4 TAGGACG 315 0.0 12.993434 4 AGGACGT 305 0.0 12.793103 5 TGTAGGA 500 0.0 12.011705 2 GTCCTAA 250 0.0 11.833188 1 GTAGGAC 460 0.0 11.812754 3 ATAAGCC 65 7.7799254E-4 11.733046 3 CAATACT 65 7.881024E-4 11.715011 4 TTAGGAC 180 0.0 11.6515665 3 GACGTGG 180 0.0 11.631669 7 TGGCGAG 340 0.0 11.435159 18 TAAGACA 75 2.0326549E-4 11.422135 4 CAGGATA 100 1.8782921E-6 11.422134 4 CTGTAGG 495 0.0 11.374358 1 TATGGCG 360 0.0 11.3266945 16 >>END_MODULE