FastQCFastQC Report
Thu 26 May 2016
SRR1926954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587914
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18210.3097391795398647No Hit
GGTATCAACGCAGAGTACTTTTTTT11420.19424609721830063No Hit
GTACATGGGAAGCAGTGGTATCAAC10300.17519569188690864No Hit
TATCAACGCAGAGTACTTTTTTTTT9450.16073779498362004No Hit
CCCATGTACTCTGCGTTGATACCAC8650.14713036260405435No Hit
GTCCTACAGTGGACATTTCTAAATT8590.1461098051755869No Hit
CTGTAGGACGTGGAATATGGCAAGA8420.14321822579492918No Hit
GTCCTACAGTGTGCATTTCTCATTT7710.1311416295580646No Hit
GTCCTAAAGTGTGTATTTCTCATTT6390.10868936613178118No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6330.10766880870331376No Hit
CTTTAGGACGTGAAATATGGCGAGG6280.1068183441795909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2350.014.9891574
TAGAGCA1009.729774E-913.3281974
TGGTACT500.001473892813.3281974
TGTAGGA6000.013.1864072
AGGACCT3650.012.7804615
GTAGGAC5850.012.6946033
CTGTAGG6000.012.3920451
GCCATAT1103.619789E-812.13207151
CTGGACC550.00301630112.1165424
GTTCTAG802.7614677E-511.9154281
GTAGAGC802.7954804E-511.9011913
GGACCTG3600.011.9001756
GGCTTAT657.785135E-411.7321141
CTACCCT657.8692805E-411.7170944
AGAAGTG907.2649764E-611.6357275
TAGCCTG907.2649764E-611.6357275
TGGCGAG3700.011.54014118
GGACGTG4150.011.2406476
AGGACGT4350.011.1615435
GTACAAT600.005721639411.1210661
Produced by FastQC (version 0.11.5)