##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926954_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 587914 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.829762516286397 31.0 30.0 33.0 26.0 34.0 2 30.115248148538733 31.0 30.0 34.0 26.0 34.0 3 30.186226216759593 31.0 30.0 34.0 26.0 34.0 4 33.753756501801284 35.0 35.0 37.0 28.0 37.0 5 33.7183227478849 37.0 35.0 37.0 28.0 37.0 6 33.66697680272965 37.0 35.0 37.0 29.0 37.0 7 33.64762023016972 37.0 35.0 37.0 28.0 37.0 8 33.668289919954276 37.0 35.0 37.0 29.0 37.0 9 35.18724847511711 39.0 35.0 39.0 29.0 39.0 10 35.083541130165294 39.0 35.0 39.0 28.0 39.0 11 35.09045030395602 39.0 35.0 39.0 29.0 39.0 12 34.98993050003912 39.0 35.0 39.0 28.0 39.0 13 34.99415900965107 39.0 35.0 39.0 28.0 39.0 14 36.09821334412856 40.0 36.0 41.0 27.0 41.0 15 36.086476933701185 40.0 36.0 41.0 28.0 41.0 16 36.06600965447328 40.0 36.0 41.0 27.0 41.0 17 36.0594967971506 40.0 36.0 41.0 28.0 41.0 18 36.03910435880078 40.0 36.0 41.0 27.0 41.0 19 36.110619920600634 40.0 37.0 41.0 27.0 41.0 20 36.06487343386958 40.0 37.0 41.0 27.0 41.0 21 36.023455811564276 40.0 37.0 41.0 27.0 41.0 22 35.92589052140279 40.0 36.0 41.0 27.0 41.0 23 35.8561269165218 40.0 36.0 41.0 27.0 41.0 24 35.7866388621465 40.0 36.0 41.0 27.0 41.0 25 35.67015754004837 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0899921037437359 1101 2 0.12846372624559166 1101 3 0.40143377488045573 1101 4 -0.05559888496075871 1101 5 -0.03552011727235538 1101 6 0.17318300420114952 1101 7 0.17659597771061186 1101 8 0.20006358021757364 1101 9 0.2480631390604202 1101 10 0.32034979611393766 1101 11 0.34840566805983286 1101 12 0.14598346645830418 1101 13 0.18166563209069864 1101 14 0.2791968515247092 1101 15 0.39168992094963784 1101 16 0.5317124800089701 1101 17 0.3096989650258237 1101 18 0.41468917514387726 1101 19 0.3652067516087385 1101 20 0.42717579456176935 1101 21 0.29085852450897676 1101 22 0.039835933790492106 1101 23 0.241026640464014 1101 24 0.23948030131992226 1101 25 0.3898262436392699 1102 1 -0.3847789996953246 1102 2 -0.4373677939493348 1102 3 -0.23286366804298453 1102 4 -0.3043887204873741 1102 5 -0.3677039403152378 1102 6 -0.18500881351713616 1102 7 -0.3725365052964875 1102 8 -0.11044431909778041 1102 9 0.0035482881918653675 1102 10 -0.13622116019818264 1102 11 -0.1830910436401325 1102 12 0.12264166100059981 1102 13 -0.03089776288285151 1102 14 -0.06611033109822273 1102 15 -0.21450420097888667 1102 16 -0.09798302950562743 1102 17 -0.17322380111629343 1102 18 -0.2287394793192945 1102 19 -0.3818231395510594 1102 20 -0.44548415408652176 1102 21 -0.08362104962729688 1102 22 -0.13381537017713185 1102 23 -0.1946533286055967 1102 24 -0.26116775781109425 1102 25 -0.3835230219585881 1103 1 -0.9954487186120389 1103 2 -0.8110210872876671 1103 3 -0.5587637200101732 1103 4 -0.6868540702133927 1103 5 -0.7234660489990219 1103 6 -0.7139413811567081 1103 7 -0.6264797623893514 1103 8 -0.8504149806598207 1103 9 -0.8237877684852606 1103 10 -0.595083539242907 1103 11 -0.804066053342865 1103 12 -0.8774609577510049 1103 13 -0.6901245700582734 1103 14 -0.8555327059329443 1103 15 -0.9804058316961033 1103 16 -0.6935085888107153 1103 17 -0.8862701966326441 1103 18 -0.9620201876518095 1103 19 -0.8909700225064796 1103 20 -0.9712539591766713 1103 21 -0.9486445006432902 1103 22 -0.9202644324653946 1103 23 -1.0715818704326026 1103 24 -1.0587167483977993 1103 25 -1.0168675381045986 1104 1 -0.13470885396915833 1104 2 -0.09990997617655495 1104 3 -0.03393291640941243 1104 4 0.10185740885881955 1104 5 0.06564624484420278 1104 6 0.05270557330810277 1104 7 -0.20201034915589133 1104 8 -0.0525898935975917 1104 9 -0.2730091365120657 1104 10 -0.0035269323699225197 1104 11 -0.17843362020008158 1104 12 -0.09876984795403843 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15 0.10825138109092336 2211 16 0.14128019358189903 2211 17 0.1417775585380312 2211 18 0.1095976215143466 2211 19 0.18542704043842662 2211 20 -0.05105142942311147 2211 21 0.14778133497878088 2211 22 -0.06845493000850666 2211 23 0.01598048593755408 2211 24 0.15614788923462442 2211 25 -0.1412290275577277 2212 1 -0.06765201739483473 2212 2 -0.007008097262151125 2212 3 -0.08741746990334676 2212 4 0.09287099052474446 2212 5 -0.22883664319501662 2212 6 -0.21523794197420187 2212 7 -0.3412095234656647 2212 8 -0.3078970644357142 2212 9 -0.42274758975032256 2212 10 -0.4433688314574411 2212 11 -0.48474827385587105 2212 12 -0.6016597637430223 2212 13 -0.509649896945426 2212 14 -0.6548990759289168 2212 15 -0.4192086494572749 2212 16 -0.4232000757047061 2212 17 -0.6090222751588144 2212 18 -0.6471695607365717 2212 19 -0.5008480578872039 2212 20 -0.5744050170579769 2212 21 -0.48936652142244697 2212 22 -0.33832960234904874 2212 23 -0.583042149522818 2212 24 -0.8182596557706106 2212 25 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12 0.070435018314285 2214 13 0.2656882718345486 2214 14 0.22538148671314673 2214 15 0.30369729527706824 2214 16 0.20325113761601443 2214 17 0.2808266575107865 2214 18 0.22117476575930084 2214 19 0.15597883298239879 2214 20 -0.09573687855045421 2214 21 -0.13731918095321305 2214 22 0.11466277792519008 2214 23 0.020605712551088118 2214 24 -0.051568200944402065 2214 25 -0.08631950640408803 2215 1 0.2859502917343413 2215 2 0.44462443677890917 2215 3 0.2021449619511415 2215 4 0.20405911017346767 2215 5 0.2117341309380052 2215 6 0.3160181330133298 2215 7 -0.10830612316307509 2215 8 -0.11899347818568629 2215 9 -0.1791184027632582 2215 10 -0.10516001247727758 2215 11 -0.2889963277468226 2215 12 -0.07428956773750883 2215 13 -0.05348489394491196 2215 14 0.1492581172789258 2215 15 0.03542862624361476 2215 16 -0.15846585376797861 2215 17 0.03059889918382197 2215 18 -0.07776467708050916 2215 19 -0.07011982007733053 2215 20 0.11556565395873264 2215 21 -0.09326888210977557 2215 22 -0.13128557506549043 2215 23 0.1648553765309586 2215 24 0.07711321111637659 2215 25 -0.018869337474818337 2216 1 -0.4017224075529633 2216 2 -0.22124465767301515 2216 3 -2.126860740451402 2216 4 -1.6017542292729914 2216 5 -0.7062615953303037 2216 6 -0.730427997089869 2216 7 -0.8590603713096954 2216 8 -0.6129277675140443 2216 9 -0.7331663717173029 2216 10 -0.6601986357134422 2216 11 -0.6497132012091598 2216 12 -0.5625194077181845 2216 13 -0.6711793303158444 2216 14 -0.8216086642818041 2216 15 -0.9676538641466266 2216 16 -0.7488057464334545 2216 17 -0.8915528674147595 2216 18 -0.8673673100922201 2216 19 -0.9426969130391782 2216 20 -0.7784015889521569 2216 21 -0.8059934679385066 2216 22 -0.6527770419297383 2216 23 -0.6672819396146608 2216 24 -0.7508353157970831 2216 25 -0.8071548505887023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14865.0 3 15.0 4 229.0 5 1719.0 6 2461.0 7 1818.0 8 1342.0 9 1172.0 10 1017.0 11 944.0 12 902.0 13 760.0 14 817.0 15 712.0 16 799.0 17 806.0 18 938.0 19 1095.0 20 1528.0 21 1986.0 22 2346.0 23 2763.0 24 3428.0 25 3928.0 26 4679.0 27 5166.0 28 5927.0 29 7179.0 30 9284.0 31 12084.0 32 15626.0 33 20212.0 34 27264.0 35 38289.0 36 56673.0 37 98576.0 38 238565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.826810914273935 19.70740645504069 10.672631383561153 27.793151247124214 2 13.68532188987373 21.29045441773736 40.74450603250828 24.279717659880625 3 18.278633304525936 25.387879117813178 29.37741999142583 26.956067586235054 4 11.43602635759652 17.934425783362872 38.477396353888494 32.15215150515212 5 12.14283041269906 39.323100056813736 35.76322620371977 12.770843326767434 6 30.7245130116727 38.89796329388954 18.06290184110859 12.31462185332917 7 27.5051719679338 32.585099288173886 21.712738012603268 18.196990731289045 8 24.44854973726693 38.77647531125661 20.056850367179884 16.718124584296575 9 25.859180038682876 16.167367233868102 20.182459339601298 37.79099338784773 10 13.709737776749629 30.791330439397445 34.6668458366786 20.83208594717433 11 33.45076052677238 23.989848302890714 24.231223272697584 18.32816789763932 12 22.38817648189443 26.23075182892707 32.13383612881498 19.247235560363524 13 28.39951148819203 22.790810807133088 25.959503867952126 22.850173836722753 14 19.828070091884186 23.647676360828285 28.12060947689628 28.40364407039125 15 22.274781555586173 31.94433813511321 23.719227700979918 22.061652608320703 16 20.854651716583433 29.990712895807537 28.49474070949394 20.659894678115094 17 20.44026922350756 30.04117956228217 28.288709383871424 21.22984183033884 18 20.19543807725672 29.26995628582606 30.32521984657516 20.20938579034206 19 22.035403197411863 28.37562700603661 28.540447311081742 21.04852248546979 20 22.06923180288106 28.63997659501726 29.21302446467055 20.07776713743113 21 21.850376287747853 27.95365468624542 28.233148705107443 21.96282032089928 22 21.535669393260566 29.321855444598093 28.309460753546865 20.83301440859447 23 20.95595614324544 28.79979044554136 28.75080368897492 21.493449722238285 24 21.486208820367676 28.25558927186382 29.445733375062932 20.812468532705573 25 21.310373921119687 28.35724419012108 28.984755340393754 21.34762654836548 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 306.0 1 306.0 2 319.5 3 333.0 4 333.0 5 333.0 6 523.5 7 714.0 8 714.0 9 714.0 10 1146.5 11 1579.0 12 1579.0 13 1579.0 14 2447.0 15 3315.0 16 3315.0 17 3315.0 18 5675.5 19 8036.0 20 8036.0 21 8036.0 22 12561.5 23 17087.0 24 17087.0 25 17087.0 26 24032.0 27 30977.0 28 30977.0 29 30977.0 30 40204.5 31 49432.0 32 49432.0 33 49432.0 34 55782.0 35 62132.0 36 62132.0 37 62132.0 38 66995.0 39 71858.0 40 71858.0 41 71858.0 42 73970.0 43 76082.0 44 76082.0 45 76082.0 46 77681.5 47 79281.0 48 79281.0 49 79281.0 50 74212.0 51 69143.0 52 69143.0 53 69143.0 54 59183.5 55 49224.0 56 49224.0 57 49224.0 58 40863.5 59 32503.0 60 32503.0 61 32503.0 62 25803.0 63 19103.0 64 19103.0 65 19103.0 66 14510.0 67 9917.0 68 9917.0 69 9917.0 70 7097.5 71 4278.0 72 4278.0 73 4278.0 74 3007.5 75 1737.0 76 1737.0 77 1737.0 78 1172.5 79 608.0 80 608.0 81 608.0 82 400.5 83 193.0 84 193.0 85 193.0 86 129.0 87 65.0 88 65.0 89 65.0 90 37.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11617345394054233 2 0.11549308232156404 3 0.01734947628394629 4 0.0 5 0.004762601332848002 6 0.3430773888698007 7 0.022112077616794294 8 0.00935510976095143 9 0.009695295570440575 10 0.003061672285402287 11 0.005442972951826287 12 1.7009290474457147E-4 13 0.001020557428467429 14 0.0 15 0.0011906503332120004 16 3.4018580948914294E-4 17 1.7009290474457147E-4 18 6.803716189782859E-4 19 1.7009290474457147E-4 20 8.504645237228575E-4 21 0.011226131713141718 22 1.7009290474457147E-4 23 0.0 24 3.4018580948914294E-4 25 0.006123344570804574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 587914.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.211424557663 #Duplication Level Percentage of deduplicated Percentage of total 1 92.27741682893996 77.70812725666299 2 5.975412533544674 10.063960035390226 3 0.9092243057051851 2.2970122207765713 4 0.2982781357522733 1.0047370690441169 5 0.13807484853395538 0.5813739845313964 6 0.09094250010906257 0.459503849221175 7 0.0545726432235398 0.32169480194119665 8 0.03732401823618627 0.2514486996708351 9 0.026626105756653976 0.20180000665717407 >10 0.16338443914901088 2.497342946321928 >50 0.01416944575851028 0.8250316172804947 >100 0.011942743363613856 1.868926100617509 >500 0.002024193790296084 1.2383936629365866 >1k 6.072581370888253E-4 0.6806477489478032 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1821 0.3097391795398647 No Hit GGTATCAACGCAGAGTACTTTTTTT 1142 0.19424609721830063 No Hit GTACATGGGAAGCAGTGGTATCAAC 1030 0.17519569188690864 No Hit TATCAACGCAGAGTACTTTTTTTTT 945 0.16073779498362004 No Hit CCCATGTACTCTGCGTTGATACCAC 865 0.14713036260405435 No Hit GTCCTACAGTGGACATTTCTAAATT 859 0.1461098051755869 No Hit CTGTAGGACGTGGAATATGGCAAGA 842 0.14321822579492918 No Hit GTCCTACAGTGTGCATTTCTCATTT 771 0.1311416295580646 No Hit GTCCTAAAGTGTGTATTTCTCATTT 639 0.10868936613178118 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 633 0.10766880870331376 No Hit CTTTAGGACGTGAAATATGGCGAGG 628 0.1068183441795909 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 1.700929047445715E-4 0.0 3.40185809489143E-4 0.0 13 0.0 1.700929047445715E-4 0.0 3.40185809489143E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 235 0.0 14.989157 4 TAGAGCA 100 9.729774E-9 13.328197 4 TGGTACT 50 0.0014738928 13.328197 4 TGTAGGA 600 0.0 13.186407 2 AGGACCT 365 0.0 12.780461 5 GTAGGAC 585 0.0 12.694603 3 CTGTAGG 600 0.0 12.392045 1 GCCATAT 110 3.619789E-8 12.1320715 1 CTGGACC 55 0.003016301 12.116542 4 GTTCTAG 80 2.7614677E-5 11.915428 1 GTAGAGC 80 2.7954804E-5 11.901191 3 GGACCTG 360 0.0 11.900175 6 GGCTTAT 65 7.785135E-4 11.732114 1 CTACCCT 65 7.8692805E-4 11.717094 4 AGAAGTG 90 7.2649764E-6 11.635727 5 TAGCCTG 90 7.2649764E-6 11.635727 5 TGGCGAG 370 0.0 11.540141 18 GGACGTG 415 0.0 11.240647 6 AGGACGT 435 0.0 11.161543 5 GTACAAT 60 0.0057216394 11.121066 1 >>END_MODULE