FastQCFastQC Report
Thu 26 May 2016
SRR1926953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670210
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14480.21605168529266944No Hit
CTGTAGGACGTGGAATATGGCAAGA12260.18292773906685963No Hit
GTCCTAAAGTGTGTATTTCTCATTT11950.17830232315244476No Hit
GTCCTACAGTGTGCATTTCTCATTT9940.14831172319123856No Hit
GTATCAACGCAGAGTACTTTTTTTT9900.14771489533131407No Hit
CTTTAGGACGTGAAATATGGCGAGG9380.13995613315229555No Hit
CTGTAGGACCTGGAATATGGCGAGA7220.10772742871637249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGGT400.005196252414.2823134
GACGTGG3450.012.1370517
CGCCTTG550.00299012412.1310522
TCTATGC550.00299012412.1310523
TAGGACC2350.011.7499884
GTCCAGA907.0954393E-611.6610321
CCTGTAC752.0076577E-411.4378493
TTAGTAC752.0076577E-411.4378493
GGACGTG6750.011.419866
TGTACTG1104.841186E-711.2468335
AGGACGT6800.011.1959435
GTCATAC600.005685187411.1309841
ACGTGGA3850.011.1149188
TTAGGCT951.32322275E-511.0249434
GTGTAGT1303.0519004E-811.0086661
GTGCTAG700.00143709310.9038221
TAGGCAG700.001467999910.8760595
TGTAGGA7100.010.8740112
GTATAGA1158.3136365E-710.7853021
CCTCTAG803.6182394E-410.733451
Produced by FastQC (version 0.11.5)