##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 670210 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.126663284642127 30.0 26.0 31.0 10.0 31.0 2 26.76917831724385 31.0 27.0 31.0 10.0 34.0 3 26.371740200832576 31.0 26.0 31.0 10.0 34.0 4 30.040590262753465 35.0 30.0 37.0 10.0 37.0 5 29.565631667686247 35.0 29.0 37.0 7.0 37.0 6 29.380920905387864 35.0 29.0 37.0 7.0 37.0 7 29.42220498052849 35.0 29.0 37.0 2.0 37.0 8 29.130964921442533 35.0 28.0 37.0 2.0 37.0 9 31.311681413288373 37.0 31.0 39.0 2.0 39.0 10 31.513767326658808 37.0 32.0 39.0 2.0 39.0 11 31.72323450858686 37.0 32.0 39.0 2.0 39.0 12 31.570182480118174 37.0 32.0 39.0 2.0 39.0 13 31.687941093090224 37.0 32.0 39.0 2.0 39.0 14 32.579883916981245 38.0 32.0 40.0 2.0 41.0 15 32.1407349935095 38.0 31.0 40.0 2.0 41.0 16 32.208503304934275 38.0 31.0 40.0 2.0 41.0 17 32.43115441428806 38.0 32.0 40.0 2.0 41.0 18 32.48267856343534 38.0 32.0 40.0 2.0 41.0 19 31.847386640008356 38.0 31.0 40.0 2.0 41.0 20 32.130500887781444 38.0 32.0 40.0 2.0 41.0 21 32.10151146655526 38.0 32.0 40.0 2.0 41.0 22 32.106668059265004 38.0 32.0 40.0 2.0 41.0 23 32.060882409990896 38.0 32.0 40.0 2.0 41.0 24 30.78046134793572 38.0 29.0 40.0 2.0 41.0 25 31.300216350099223 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.1781158353845846 1101 2 -1.2102744836378605 1101 3 -2.257810936179375 1101 4 -1.5685817540688802 1101 5 -1.7379672168844635 1101 6 -3.748340915102304 1101 7 -2.109670652259929 1101 8 -1.4703033598396615 1101 9 -1.6478001741952966 1101 10 -10.470344867762797 1101 11 -5.511630779528886 1101 12 -3.193358141702941 1101 13 -2.4489767082137703 1101 14 -2.293446797397607 1101 15 -3.7796443844330483 1101 16 -2.062649330876063 1101 17 -1.5950080049142166 1101 18 -1.3538104523529988 1101 19 -2.252276751160405 1101 20 -1.4486397750241018 1101 21 -1.5138531851937174 1101 22 -1.6734454434948596 1101 23 -1.8946379596101508 1101 24 -1.7530133826676284 1101 25 -1.7157241419141833 1102 1 -0.8671805723713497 1102 2 -0.726032100417541 1102 3 -1.01096143398264 1102 4 -1.0357876563296067 1102 5 -1.1706448118403863 1102 6 -1.7298030250333447 1102 7 -0.9955707388165749 1102 8 -0.16274011400648192 1102 9 -0.3714209084089646 1102 10 -0.39177009881981206 1102 11 -0.5238738273073338 1102 12 -0.5813688156551322 1102 13 -0.5546701664183971 1102 14 -0.8326290627203896 1102 15 -0.0186453320437181 1102 16 -0.3707715029145753 1102 17 -0.4103110967504051 1102 18 -0.7997484433363411 1102 19 -0.4545536831990802 1102 20 -0.6572925680142454 1102 21 -0.820781613923959 1102 22 -0.7360421018340375 1102 23 -1.084620566236861 1102 24 -4.080156480082824 1102 25 -2.1031030754214513 1103 1 -1.1234851915973145 1103 2 -1.015477112334466 1103 3 -0.7070685727864081 1103 4 -0.49046930426968416 1103 5 -1.1749576026700304 1103 6 -0.1369985760491197 1103 7 -0.45178456244612875 1103 8 -0.25963036482931656 1103 9 -0.41509359028389525 1103 10 -0.41315246177521203 1103 11 -0.754347099353641 1103 12 -0.745061926539254 1103 13 -0.6046417927945171 1103 14 -0.5024281774633224 1103 15 -0.4667442992438069 1103 16 -0.43489589288166286 1103 17 -0.7874079075550817 1103 18 -0.84254721759579 1103 19 -2.0120613440775266 1103 20 -1.0330501437718205 1103 21 -0.8320289184296925 1103 22 -0.7693867846169233 1103 23 -0.8387233922498005 1103 24 0.5802225915322019 1103 25 -0.1469459990396551 1104 1 -0.9728738146701481 1104 2 -0.7596527314852572 1104 3 -1.8481095686436895 1104 4 -0.9362819490892775 1104 5 -0.9812930525010124 1104 6 -2.37884705086681 1104 7 -0.688018801560208 1104 8 -0.469844722908622 1104 9 -0.559613265687382 1104 10 -0.30977308709494267 1104 11 -0.37091897501191085 1104 12 -0.4349910088218607 1104 13 -0.5578491393484377 1104 14 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-0.03836596233503897 1107 12 0.04621976647154469 1107 13 -0.22677377544523125 1107 14 0.007885332273815493 1107 15 -1.3591670861910892 1107 16 -0.5473460427957875 1107 17 -0.36919697983406863 1107 18 -0.3837139279267632 1107 19 0.49882853453569354 1107 20 0.7792631913080825 1107 21 0.36554625695528387 1107 22 0.13814385317307654 1107 23 -0.322750988092956 1107 24 -3.8234044570516197 1107 25 -1.9973549880653572 1108 1 0.6129785713970577 1108 2 0.25068597627760525 1108 3 0.8303405033981761 1108 4 0.6589961962162256 1108 5 0.8936291393860998 1108 6 -2.482800619810348 1108 7 -0.932984315074453 1108 8 0.6389148541240637 1108 9 0.44395167284300285 1108 10 0.8844533337897715 1108 11 0.607018745685675 1108 12 0.8814981347146826 1108 13 0.6900868345871736 1108 14 0.5878978230667613 1108 15 -0.5451846797250042 1108 16 0.7783443815831035 1108 17 0.6062723351047552 1108 18 0.7338199526489291 1108 19 0.5558156768607141 1108 20 -0.5719493398138731 1108 21 0.20866077736204147 1108 22 0.7530857541821447 1108 23 0.6428209137843055 1108 24 -0.39292580202435445 1108 25 0.5272331098333325 1109 1 0.8806248745817165 1109 2 0.6893469907655003 1109 3 -0.7232127734133691 1109 4 0.49113989113261525 1109 5 0.9324962461631259 1109 6 1.0822804208160832 1109 7 1.1015766357426564 1109 8 1.743748248894125 1109 9 0.7678740168216436 1109 10 0.8591916691896131 1109 11 0.5614662061148401 1109 12 0.12895409575262917 1109 13 0.4838465387972626 1109 14 0.5361820628710561 1109 15 -0.8813385172862986 1109 16 0.33600274870663327 1109 17 0.34063110324255064 1109 18 0.4065297590673964 1109 19 -1.006592866195259 1109 20 -0.10330441057781314 1109 21 0.4679014194316835 1109 22 0.6348451903047092 1109 23 0.6323319891504084 1109 24 -0.24940123291920813 1109 25 0.16672519566602162 1110 1 0.12796950985738675 1110 2 0.41617726931991683 1110 3 0.5360440203261838 1110 4 0.5872655734651957 1110 5 0.3728466951342675 1110 6 0.4355141964636502 1110 7 0.4552763946148275 1110 8 0.7403384351394813 1110 9 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2215 7 1.2589443482827818 2215 8 0.004688499489546416 2215 9 0.3790369038466004 2215 10 0.4379531893304396 2215 11 0.14647190146536104 2215 12 0.0810095995322726 2215 13 0.1348613467086217 2215 14 0.4596520846169412 2215 15 0.11256228006277524 2215 16 0.33911448112871057 2215 17 -0.026501546648717067 2215 18 -0.799631574680145 2215 19 0.19146035944417505 2215 20 -3.6335415442632204 2215 21 -1.502244588645361 2215 22 -0.7224034376335773 2215 23 -0.7646857182121245 2215 24 -0.6000121887025784 2215 25 -0.4172708693645255 2216 1 0.9264438357569169 2216 2 -0.02696918629253986 2216 3 0.805480306761563 2216 4 0.5750489791996429 2216 5 0.8104449267034859 2216 6 -0.8050792009747134 2216 7 -8.727360814540965 2216 8 -9.38471713461237 2216 9 -4.5147703213296175 2216 10 -2.2964844214444327 2216 11 -1.3662787458274437 2216 12 -1.2045424983498343 2216 13 -0.8985683658688686 2216 14 -0.7465447740731186 2216 15 -1.9469919250943342 2216 16 -1.069437134857175 2216 17 -1.2837475505855664 2216 18 -0.9537068648388463 2216 19 -0.4219682096507569 2216 20 -1.1487621138808883 2216 21 -3.6077855784111925 2216 22 -2.425022012690956 2216 23 -2.3228685610731183 2216 24 -2.6209019814872043 2216 25 -1.902588553322282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61070.0 3 12.0 4 458.0 5 2271.0 6 2803.0 7 2373.0 8 1861.0 9 1764.0 10 1632.0 11 1577.0 12 1536.0 13 1501.0 14 1550.0 15 1608.0 16 1765.0 17 1838.0 18 2241.0 19 2678.0 20 3463.0 21 4358.0 22 5445.0 23 6552.0 24 7287.0 25 8553.0 26 9397.0 27 10430.0 28 11628.0 29 13592.0 30 15697.0 31 19265.0 32 24244.0 33 31315.0 34 41909.0 35 57076.0 36 79387.0 37 116848.0 38 113226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87056899289245 19.143595279174004 13.31298047740504 25.67285525052851 2 13.793448578752695 20.12512473633577 42.605749523462386 23.475677161449152 3 19.27085200721858 24.597241643062876 29.36998792920058 26.761918420517965 4 11.666671643618404 16.916360335287283 38.88681515899868 32.53015286209563 5 12.22931609313765 37.99021583077387 36.05681265315747 13.723655422931014 6 30.57678072542324 37.56954559564459 19.579174526612384 12.274499152319779 7 26.842291139164736 31.187410586552218 23.981509747936382 17.988788526346664 8 24.83293381096457 37.580839685290094 21.213440710581207 16.372785793164127 9 25.818968154596234 15.834055882796259 21.04521692153635 37.30175904107115 10 14.222833850016196 29.63898868471997 35.32051004583725 20.81766741942658 11 33.4761476255072 23.068462813814634 25.258580422951166 18.196809137726998 12 22.857974301959437 25.255861152979453 32.256413317614054 19.62975122744706 13 28.3414862723211 22.956815787353047 25.833972535688382 22.867725404637476 14 19.816145471910698 23.233571359284877 28.358964027488643 28.591319141315786 15 22.30915705873399 32.44367309052598 23.393015433379198 21.854154417360835 16 20.870207590251766 28.987120748578505 29.22575253331742 20.916919127852314 17 20.694569636045628 29.666280645411618 28.62760489729646 21.011544821246293 18 20.476699278325285 28.530177225163705 30.77190542420028 20.22121807231073 19 23.319523377429675 27.450869191460907 27.658764745212284 21.57084268589713 20 22.743261010038903 28.21399417692768 28.917030919588772 20.12571389344464 21 22.017528543948952 27.611560541823916 27.58395301048937 22.78695790373776 22 22.144007306470314 29.383024096727162 27.893823376228006 20.579145220574517 23 21.109215806541638 28.944733217227274 28.51791659764475 21.428134378586343 24 22.93409581342319 27.58553277599859 28.92325554325214 20.557115867326075 25 21.661192334841076 28.467371141789922 28.881191827451847 20.99024469591716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 217.0 2 271.5 3 326.0 4 326.0 5 326.0 6 552.5 7 779.0 8 779.0 9 779.0 10 1197.0 11 1615.0 12 1615.0 13 1615.0 14 2574.0 15 3533.0 16 3533.0 17 3533.0 18 6181.5 19 8830.0 20 8830.0 21 8830.0 22 13839.5 23 18849.0 24 18849.0 25 18849.0 26 27102.5 27 35356.0 28 35356.0 29 35356.0 30 45933.5 31 56511.0 32 56511.0 33 56511.0 34 64213.0 35 71915.0 36 71915.0 37 71915.0 38 76940.5 39 81966.0 40 81966.0 41 81966.0 42 84783.5 43 87601.0 44 87601.0 45 87601.0 46 87375.0 47 87149.0 48 87149.0 49 87149.0 50 81581.5 51 76014.0 52 76014.0 53 76014.0 54 65887.5 55 55761.0 56 55761.0 57 55761.0 58 47188.5 59 38616.0 60 38616.0 61 38616.0 62 30873.0 63 23130.0 64 23130.0 65 23130.0 66 17768.0 67 12406.0 68 12406.0 69 12406.0 70 9087.5 71 5769.0 72 5769.0 73 5769.0 74 4088.0 75 2407.0 76 2407.0 77 2407.0 78 1681.5 79 956.0 80 956.0 81 956.0 82 641.5 83 327.0 84 327.0 85 327.0 86 218.5 87 110.0 88 110.0 89 110.0 90 76.0 91 42.0 92 42.0 93 42.0 94 31.0 95 20.0 96 20.0 97 20.0 98 12.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20053416093463242 2 0.11906715805493799 3 0.12324495307440952 4 0.06803837603139315 5 0.022082630817206548 6 0.021784216887244297 7 0.29334089315289236 8 0.2852837170439116 9 0.019247698482565165 10 0.03446680891063995 11 0.016263559182942658 12 0.01790483579773504 13 0.015517524358037034 14 0.017158800972829413 15 0.015517524358037034 16 0.020888975097357546 17 0.01880007758762179 18 0.015219110428074783 19 0.0241715283269423 20 0.017457214902791663 21 0.015368317393055908 22 0.017755628832753913 23 0.019844526342489666 24 0.017308007937810538 25 0.01686038704286716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 670210.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.25031647699267 #Duplication Level Percentage of deduplicated Percentage of total 1 94.09295652942208 82.0964023544799 2 4.57539728194066 7.984097217145891 3 0.6924487170833179 1.8124910912882115 4 0.2188085127794272 0.7636444795146047 5 0.09736677270012824 0.4247640866214799 6 0.06437175928333623 0.33698738217911256 7 0.04540063051764173 0.27728535666434767 8 0.03526944946051184 0.2461816501919165 9 0.02550552054954231 0.20028282658722057 >10 0.12782854360535129 2.0935119594054354 >50 0.013863685037279883 0.8612083831511078 >100 0.009242226533420304 1.6203902582032783 >500 0.0010269140581755563 0.7049914666488065 >1k 5.134570290877781E-4 0.5777614879187105 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1448 0.21605168529266944 No Hit CTGTAGGACGTGGAATATGGCAAGA 1226 0.18292773906685963 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1195 0.17830232315244476 No Hit GTCCTACAGTGTGCATTTCTCATTT 994 0.14831172319123856 No Hit GTATCAACGCAGAGTACTTTTTTTT 990 0.14771489533131407 No Hit CTTTAGGACGTGAAATATGGCGAGG 938 0.13995613315229555 No Hit CTGTAGGACCTGGAATATGGCGAGA 722 0.10772742871637249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.4920696498112531E-4 10 0.0 0.0 0.0 1.4920696498112531E-4 1.4920696498112531E-4 11 0.0 0.0 0.0 1.4920696498112531E-4 2.9841392996225063E-4 12 0.0 0.0 0.0 1.4920696498112531E-4 2.9841392996225063E-4 13 0.0 2.9841392996225063E-4 0.0 1.4920696498112531E-4 2.9841392996225063E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAAGGT 40 0.0051962524 14.282313 4 GACGTGG 345 0.0 12.137051 7 CGCCTTG 55 0.002990124 12.131052 2 TCTATGC 55 0.002990124 12.131052 3 TAGGACC 235 0.0 11.749988 4 GTCCAGA 90 7.0954393E-6 11.661032 1 CCTGTAC 75 2.0076577E-4 11.437849 3 TTAGTAC 75 2.0076577E-4 11.437849 3 GGACGTG 675 0.0 11.41986 6 TGTACTG 110 4.841186E-7 11.246833 5 AGGACGT 680 0.0 11.195943 5 GTCATAC 60 0.0056851874 11.130984 1 ACGTGGA 385 0.0 11.114918 8 TTAGGCT 95 1.32322275E-5 11.024943 4 GTGTAGT 130 3.0519004E-8 11.008666 1 GTGCTAG 70 0.001437093 10.903822 1 TAGGCAG 70 0.0014679999 10.876059 5 TGTAGGA 710 0.0 10.874011 2 GTATAGA 115 8.3136365E-7 10.785302 1 CCTCTAG 80 3.6182394E-4 10.73345 1 >>END_MODULE