FastQCFastQC Report
Thu 26 May 2016
SRR1926953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670210
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17200.25663597976753555No Hit
CTGTAGGACGTGGAATATGGCAAGA15700.23425493502036676No Hit
GTCCTACAGTGGACATTTCTAAATT15300.22828665642112173No Hit
CTTTAGGACGTGAAATATGGCGAGG12310.18367377389176529No Hit
GTCCTAAAGTGTGTATTTCTCATTT12240.1826293251368974No Hit
GGTATCAACGCAGAGTACTTTTTTT10640.15875621073991733No Hit
GTCCTACAGTGTGCATTTCTCATTT10620.1584577968099551No Hit
TATCAACGCAGAGTACTTTTTTTTT8770.13085450828844689No Hit
CTGTAGGACCTGGAATATGGCGAGA8370.12488622968920189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG504.609188E-617.0785818
TAGGACC3650.013.8246864
CCCCTAG603.9816706E-412.7057791
CTGTAGG9550.012.1735991
CCTAGGC550.003013864912.1182573
CGGTTTC550.003092493612.07666613
TGTAGGA9900.012.032892
GTAGGAC9600.012.0010413
GTCCTAC8050.011.9560591
GCTTAAT657.808299E-411.7284121
ATAGGAC657.862476E-411.7187523
AGGACGT8050.011.7087785
GTATAGG907.1915038E-611.6469651
AGGACCT6250.011.577265
TCGAACT752.0947025E-411.3857219
GGACGTG8400.011.2209126
CGTGGAA4000.011.1510149
TAGGACG8850.011.0806754
GCCGGTT600.00591818511.07110211
CCGGTTT600.005921473311.07027612
Produced by FastQC (version 0.11.5)