FastQCFastQC Report
Thu 26 May 2016
SRR1926952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656271
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16110.24547785899422647No Hit
GTCCTACAGTGTGCATTTCTCATTT13880.2114979939689549No Hit
CTGTAGGACGTGGAATATGGCAAGA13850.21104086573991537No Hit
GTCCTAAAGTGTGTATTTCTCATTT11600.17675624856195077No Hit
CTTTAGGACGTGAAATATGGCGAGG9000.13713846871185836No Hit
CTGTAGGACCTGGAATATGGCGAGA8260.12586263906221667No Hit
GTATCAACGCAGAGTACTTTTTTTT7990.12174848500086093No Hit
GTACATGGGAAGCAGTGGTATCAAC6850.10437761229735887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG502.0956031E-719.0618112
GCCGGTT402.7960766E-416.59519411
TCGCCTT551.0826878E-515.6103751
GTATTAA1051.2823875E-913.6281041
TTTATAC801.9245745E-613.10399153
CCTACAC1051.8993887E-812.7069023
GTGCTAC701.04747276E-412.2652941
GTCTTAG701.04747276E-412.2652941
TAGGACC3150.012.0897734
ATCAATC550.003104894412.07015119
CTCGAAC550.003104894412.07015118
AATGTCC1501.2732926E-1112.0457248
GGCGAGG3000.012.01267419
CTACACT1353.5652192E-1011.9890254
AGGACGT7050.011.7435535
TCTACAC1302.4738256E-911.7294473
TCCTACA9250.011.643162
GTCCTAC8850.011.6416351
TAGGACG7400.011.4505664
GTACATA1001.8296687E-611.4476081
Produced by FastQC (version 0.11.5)