FastQCFastQC Report
Thu 26 May 2016
SRR1926952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656271
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA16110.24547785899422647No Hit
GTCCTACAGTGGACATTTCTAAATT15940.2428874656963358No Hit
GTCCTACAGTGTGCATTTCTCATTT14260.21728828487012228No Hit
GTATCAACGCAGAGTACTTTTTTTT13970.2128693786560735No Hit
GTCCTAAAGTGTGTATTTCTCATTT11530.17568961602752522No Hit
CTTTAGGACGTGAAATATGGCGAGG11000.16761368398116022No Hit
CTGTAGGACCTGGAATATGGCGAGA10190.15527122179709296No Hit
GGTATCAACGCAGAGTACTTTTTTT8700.1325671864214631No Hit
GTACATGGGAAGCAGTGGTATCAAC8570.13058629742895847No Hit
CCCATGTACTCTGCGTTGATACCAC7170.10925364674044716No Hit
TATCAACGCAGAGTACTTTTTTTTT7170.10925364674044716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAC508.448922E-515.2526071
CCAACGA400.005314408314.2339419
TGCTATA655.280805E-513.1963412
TAGGACC3950.013.0153534
CTTAGCC751.4397605E-512.69689753
GCACTAA550.002986869512.1327551
TCCAACG550.003091191612.07728318
AGGACCT5700.011.6918295
GTCTAAA600.005720447311.1216931
GACAGTC600.00592235411.0699997
GGACCTG5850.011.0665516
TGTAGGA10600.011.0591972
CCTACAC1657.6397555E-1110.9655023
CTAAATT4950.010.92706619
GTAGGAC9600.010.9113973
GTTGTAG700.00144707910.8947191
GTTCAGG700.00144707910.8947191
GTCTTAG1053.3154483E-610.8947181
GTAGCAC700.001460073910.8830563
CATTAGA803.6460767E-410.7244881