##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926952_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 656271 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.659524799968306 31.0 30.0 33.0 25.0 34.0 2 29.98984718203303 31.0 30.0 33.0 26.0 34.0 3 30.063734341453454 31.0 30.0 34.0 26.0 34.0 4 33.6793443562187 35.0 35.0 37.0 28.0 37.0 5 33.66921744218471 37.0 35.0 37.0 28.0 37.0 6 33.6248790514894 37.0 35.0 37.0 29.0 37.0 7 33.59742545381405 37.0 35.0 37.0 28.0 37.0 8 33.62378041997894 37.0 35.0 37.0 28.0 37.0 9 35.136379635851654 39.0 35.0 39.0 29.0 39.0 10 35.02284879264816 39.0 35.0 39.0 28.0 39.0 11 35.07128305227566 39.0 35.0 39.0 29.0 39.0 12 34.96520187544475 39.0 35.0 39.0 28.0 39.0 13 34.9752510776798 39.0 35.0 39.0 28.0 39.0 14 36.04875577314859 40.0 36.0 41.0 27.0 41.0 15 36.03769631752736 40.0 36.0 41.0 27.0 41.0 16 36.01858073874969 40.0 36.0 41.0 27.0 41.0 17 35.99415485371135 40.0 36.0 41.0 27.0 41.0 18 35.96096582052231 40.0 36.0 41.0 27.0 41.0 19 36.02837242541572 40.0 36.0 41.0 27.0 41.0 20 35.986269391760416 40.0 36.0 41.0 27.0 41.0 21 35.93691478063178 40.0 36.0 41.0 27.0 41.0 22 35.84550741995304 40.0 36.0 41.0 27.0 41.0 23 35.769601277521026 40.0 36.0 41.0 27.0 41.0 24 35.70261980188062 40.0 36.0 41.0 27.0 41.0 25 35.578492726327994 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3415039631522525 1101 2 0.25959328666329284 1101 3 0.4549872443195113 1101 4 0.3214246083368053 1101 5 0.38248197644689697 1101 6 0.18079262157317544 1101 7 0.4074604906548984 1101 8 0.5061476092278667 1101 9 0.46278882000017063 1101 10 0.6259298322749274 1101 11 0.45521694705266214 1101 12 0.3045628149198265 1101 13 0.3060461581129843 1101 14 0.5524019493989059 1101 15 0.5519880996253903 1101 16 0.4618213651509322 1101 17 0.6675714240944615 1101 18 0.5670459252351918 1101 19 0.6073185641709813 1101 20 0.5516177155148938 1101 21 0.32807166644141716 1101 22 0.42559518446407907 1101 23 0.40658022862402987 1101 24 0.39760572597336363 1101 25 0.27846551955146737 1102 1 -0.4796011935567819 1102 2 -0.5086869737556832 1102 3 -0.40171736320665374 1102 4 -0.47590624481583177 1102 5 -0.4033042260716684 1102 6 -0.40972068725361055 1102 7 -0.4464686765653809 1102 8 -0.3011305400849196 1102 9 -0.2305781729283609 1102 10 -0.3385799629625339 1102 11 -0.31880906531871744 1102 12 -0.1761788772129833 1102 13 -0.2617498765504891 1102 14 -0.34963977078356834 1102 15 -0.3558052777016272 1102 16 -0.4572745499324 1102 17 -0.5216693158261165 1102 18 -0.4661235390162517 1102 19 -0.5689514212136686 1102 20 -0.36488286762283195 1102 21 -0.5669871640352753 1102 22 -0.4515295774828658 1102 23 -0.6767272694936892 1102 24 -0.4510028631286005 1102 25 -0.5191044938828426 1103 1 -0.5118900835633191 1103 2 -0.6830018136080334 1103 3 -0.3854486618922017 1103 4 -0.4482325892247445 1103 5 -0.40002721240445993 1103 6 -0.42671520321118095 1103 7 -0.3102788287004685 1103 8 -0.15847246090997658 1103 9 -0.4222247958686367 1103 10 -0.3049045303089599 1103 11 -0.05008704147925869 1103 12 -0.25209060789629945 1103 13 -0.1828066863387292 1103 14 -0.2099894832682665 1103 15 -0.397902527002195 1103 16 -0.039605696946352964 1103 17 -0.14156038445997865 1103 18 -0.2513208649415404 1103 19 -0.09317696416937338 1103 20 -0.1281489546239456 1103 21 -0.22862342242135014 1103 22 -0.30562012483191126 1103 23 -0.3826441380768628 1103 24 -0.37675675871188474 1103 25 -0.4894496404411868 1104 1 0.18488585608281127 1104 2 0.13470872742356121 1104 3 0.11123892007248415 1104 4 0.38235349682847897 1104 5 0.3856904848033196 1104 6 0.1663260323635356 1104 7 0.093250105455148 1104 8 0.13247406080233048 1104 9 0.17136867521511334 1104 10 0.25473192957286983 1104 11 0.1985005291422155 1104 12 0.335515834254295 1104 13 0.2781229001512955 1104 14 0.19809265753487182 1104 15 0.3622571138357742 1104 16 0.40054689915754693 1104 17 0.3363949710304368 1104 18 0.39615487152172335 1104 19 0.44674419053020387 1104 20 0.4595928101018387 1104 21 0.3489820844114462 1104 22 0.4324680059852142 1104 23 0.47705122692592994 1104 24 0.2892939912338903 1104 25 0.5346248426962248 1105 1 0.07252464101295075 1105 2 -0.017245177267056988 1105 3 0.18261764699364136 1105 4 0.21857168545123073 1105 5 0.295215185402121 1105 6 0.10475581342640794 1105 7 -0.13845835262868178 1105 8 -0.38903565414278063 1105 9 -0.31128125929087247 1105 10 -0.11923108190417508 1105 11 -0.1289734291911202 1105 12 0.12934029470838482 1105 13 0.008716400650300216 1105 14 0.10557261948596874 1105 15 0.09694204085673164 1105 16 0.02612581944197956 1105 17 0.09756403914420986 1105 18 0.19088182386703068 1105 19 -0.08636147314245335 1105 20 0.010266852641755975 1105 21 0.17276810611803484 1105 22 -0.0427787660956227 1105 23 0.14888919162154224 1105 24 0.09195429562510782 1105 25 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-0.4658968195844011 2215 20 -0.4751724907571173 2215 21 -0.4041352763725996 2215 22 -0.4897773908615406 2215 23 -0.4144410693803309 2215 24 -0.3532411631763921 2215 25 -0.45009672540353307 2216 1 -0.4521492220259553 2216 2 -0.34547286522709797 2216 3 -2.1620945391919513 2216 4 -1.8248283539809442 2216 5 -0.896310182631531 2216 6 -0.4899953941839996 2216 7 -0.35079240888525476 2216 8 -0.4629124532358375 2216 9 -0.627698804966478 2216 10 -0.612687805090296 2216 11 -0.7299957905213006 2216 12 -0.6015689866401956 2216 13 -0.6252092368365538 2216 14 -0.6498297940918434 2216 15 -0.7108805225633503 2216 16 -0.590908675497559 2216 17 -0.7930999822806086 2216 18 -0.6739541218480056 2216 19 -0.9175178217228961 2216 20 -0.7835675568613993 2216 21 -0.570834433971342 2216 22 -0.6049429764662833 2216 23 -0.6238982935913882 2216 24 -0.7364746607366186 2216 25 -0.8812720701027814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16996.0 3 18.0 4 259.0 5 1916.0 6 2570.0 7 1913.0 8 1462.0 9 1203.0 10 1088.0 11 1062.0 12 985.0 13 890.0 14 823.0 15 872.0 16 910.0 17 997.0 18 1078.0 19 1258.0 20 1659.0 21 2095.0 22 2659.0 23 3205.0 24 3907.0 25 4612.0 26 5441.0 27 5942.0 28 6832.0 29 8353.0 30 10608.0 31 13741.0 32 17809.0 33 23227.0 34 31543.0 35 44302.0 36 64571.0 37 112598.0 38 256867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.619171695845054 19.536146011947984 10.35273406274218 28.491948229464782 2 13.785929456625357 20.927969494756912 40.877178265014294 24.408922783603433 3 19.02244398148642 24.554572235421777 28.79785176639483 27.62513201669697 4 11.847239935941097 17.32241711122387 38.29530788348106 32.535035069353974 5 12.40182737465752 38.94173186111221 35.85262753679283 12.803813227437438 6 31.6128667274184 38.499727840398265 17.645269009889486 12.242136422293846 7 28.313906703406573 31.62598453997903 21.466172059791752 18.59393669682265 8 24.633875750916644 39.36479538188169 19.478267230317677 16.52306163688399 9 25.90704053642182 15.8849436147516 19.924718073758 38.283297775068576 10 14.084633670608316 30.468273802267465 34.520449835426064 20.92664269169816 11 34.2675064116142 22.945426229483214 24.447031609296115 18.340035749606464 12 23.06957381675446 25.31423947874874 32.120310788139584 19.495875916357214 13 29.138622777758304 22.444203010073704 25.666843932996375 22.750330279171614 14 19.866183329752495 23.434831037787742 27.549899355601575 29.14908627685819 15 22.18820761473454 32.6542771548821 23.004659781121333 22.152855449262024 16 20.954885740102732 29.28945078756055 28.85197640594453 20.903687066392184 17 20.635561582885092 29.933411553171712 28.371402014414798 21.059624849528397 18 20.627795174205545 28.392341508384572 30.66520384295978 20.31465947445011 19 22.849436969539976 27.55542688222835 27.75793499626678 21.83720115196489 20 22.76241645917966 28.347804091633577 28.596636120109835 20.29314332907693 21 21.904631280574225 27.460644020786663 27.566406071412242 23.06831862722687 22 21.992591475168034 29.537035767236404 27.496872481032987 20.97350027656258 23 21.080011153928787 29.015452457902303 28.241534366138378 21.663002022030533 24 22.40739329848995 27.640148109771896 29.123074344400933 20.829384247337224 25 21.60239667683568 28.4865948842043 28.5176809774443 21.39332746151572 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 247.0 3 247.0 4 247.0 5 247.0 6 408.0 7 569.0 8 569.0 9 569.0 10 914.5 11 1260.0 12 1260.0 13 1260.0 14 2077.5 15 2895.0 16 2895.0 17 2895.0 18 5114.5 19 7334.0 20 7334.0 21 7334.0 22 11862.5 23 16391.0 24 16391.0 25 16391.0 26 24002.5 27 31614.0 28 31614.0 29 31614.0 30 42516.0 31 53418.0 32 53418.0 33 53418.0 34 60760.5 35 68103.0 36 68103.0 37 68103.0 38 73401.5 39 78700.0 40 78700.0 41 78700.0 42 82467.5 43 86235.0 44 86235.0 45 86235.0 46 87793.5 47 89352.0 48 89352.0 49 89352.0 50 84304.5 51 79257.0 52 79257.0 53 79257.0 54 68314.5 55 57372.0 56 57372.0 57 57372.0 58 48155.5 59 38939.0 60 38939.0 61 38939.0 62 31277.0 63 23615.0 64 23615.0 65 23615.0 66 17810.5 67 12006.0 68 12006.0 69 12006.0 70 8733.0 71 5460.0 72 5460.0 73 5460.0 74 3779.5 75 2099.0 76 2099.0 77 2099.0 78 1445.5 79 792.0 80 792.0 81 792.0 82 532.5 83 273.0 84 273.0 85 273.0 86 170.0 87 67.0 88 67.0 89 67.0 90 44.0 91 21.0 92 21.0 93 21.0 94 12.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11626294625238659 2 0.098434945319845 3 0.01584711194003697 4 0.0 5 0.004418906214048769 6 0.34177953924522037 7 0.01935176169600668 8 0.011123453573295179 9 0.01081870142060216 10 0.004723658366741788 11 0.0060950430538603715 12 6.095043053860372E-4 13 0.0012190086107720744 14 0.0 15 0.002590393297890658 16 6.095043053860372E-4 17 1.523760763465093E-4 18 9.142564580790557E-4 19 1.523760763465093E-4 20 7.618803817325464E-4 21 0.012342462184067253 22 0.0 23 0.0 24 1.523760763465093E-4 25 0.004418906214048769 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 656271.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.0953777095638 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2663187447784 76.6690460596837 2 5.966053402971758 9.915029219107335 3 0.9183381675161291 2.289289706845843 4 0.2939686498598133 0.9770974397948681 5 0.13179217491673403 0.5475660276935458 6 0.0878791500680876 0.4381410700621907 7 0.06162231993902725 0.3584370965471138 8 0.04506096277388161 0.29954861773218294 9 0.029299371919420995 0.21911781386675477 >10 0.16410602544079977 2.562565649145394 >50 0.01842554979183719 1.0518472060915633 >100 0.01474019787143701 2.5784461663211364 >500 0.0011055148397122706 0.669988742982296 >1k 0.0012897673129976489 1.4238791841259948 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1611 0.24547785899422647 No Hit GTCCTACAGTGGACATTTCTAAATT 1594 0.2428874656963358 No Hit GTCCTACAGTGTGCATTTCTCATTT 1426 0.21728828487012228 No Hit GTATCAACGCAGAGTACTTTTTTTT 1397 0.2128693786560735 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1153 0.17568961602752522 No Hit CTTTAGGACGTGAAATATGGCGAGG 1100 0.16761368398116022 No Hit CTGTAGGACCTGGAATATGGCGAGA 1019 0.15527122179709296 No Hit GGTATCAACGCAGAGTACTTTTTTT 870 0.1325671864214631 No Hit GTACATGGGAAGCAGTGGTATCAAC 857 0.13058629742895847 No Hit CCCATGTACTCTGCGTTGATACCAC 717 0.10925364674044716 No Hit TATCAACGCAGAGTACTTTTTTTTT 717 0.10925364674044716 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.523760763465093E-4 0.0 5 0.0 0.0 0.0 1.523760763465093E-4 0.0 6 0.0 0.0 0.0 1.523760763465093E-4 0.0 7 0.0 0.0 0.0 1.523760763465093E-4 0.0 8 0.0 0.0 0.0 1.523760763465093E-4 0.0 9 0.0 0.0 0.0 3.047521526930186E-4 0.0 10 0.0 0.0 0.0 3.047521526930186E-4 0.0 11 0.0 0.0 0.0 3.047521526930186E-4 0.0 12 0.0 0.0 0.0 3.047521526930186E-4 0.0 13 0.0 0.0 0.0 3.047521526930186E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTTAC 50 8.448922E-5 15.252607 1 CCAACGA 40 0.0053144083 14.23394 19 TGCTATA 65 5.280805E-5 13.196341 2 TAGGACC 395 0.0 13.015353 4 CTTAGCC 75 1.4397605E-5 12.6968975 3 GCACTAA 55 0.0029868695 12.132755 1 TCCAACG 55 0.0030911916 12.077283 18 AGGACCT 570 0.0 11.691829 5 GTCTAAA 60 0.0057204473 11.121693 1 GACAGTC 60 0.005922354 11.069999 7 GGACCTG 585 0.0 11.066551 6 TGTAGGA 1060 0.0 11.059197 2 CCTACAC 165 7.6397555E-11 10.965502 3 CTAAATT 495 0.0 10.927066 19 GTAGGAC 960 0.0 10.911397 3 GTTGTAG 70 0.001447079 10.894719 1 GTTCAGG 70 0.001447079 10.894719 1 GTCTTAG 105 3.3154483E-6 10.894718 1 GTAGCAC 70 0.0014600739 10.883056 3 CATTAGA 80 3.6460767E-4 10.724488 1 >>END_MODULE