FastQCFastQC Report
Thu 26 May 2016
SRR1926951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680521
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19520.2868390541952416No Hit
CTGTAGGACGTGGAATATGGCAAGA16420.24128572079333332No Hit
GTCCTACAGTGTGCATTTCTCATTT15810.23232200034973205No Hit
GTCCTAAAGTGTGTATTTCTCATTT14070.2067533551499513No Hit
CTTTAGGACGTGAAATATGGCGAGG10830.15914277443311817No Hit
CTGTAGGACCTGGAATATGGCGAGA10490.15414660238258626No Hit
GTATCAACGCAGAGTACTTTTTTTT8420.123728731369054No Hit
GTACATGGGAAGCAGTGGTATCAAC7880.11579363458291515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTT250.00597811419.0277146
GTACCGT400.005224284714.2707846
CGGTTTC400.005329931614.22779213
CCCCTAT500.00145390513.35285951
TAGGACC4050.012.6898184
AATGCCG905.4880184E-712.64785518
TCCAACG604.1383313E-412.64785518
TATGACT701.07074404E-412.2366094
ACTGTTC2500.012.1687678
GATATAC2300.012.0258671
GGACCTG5800.011.9743366
AGGACCT6100.011.853335
GGCGAGG3400.011.71786519
GTAGGAC9850.011.7060533
TAATTCC752.03571E-411.4208364
TACACTG4250.011.4166285
GTCCTAC10500.011.2636371
TATACAC3300.011.2619093
GTATGAC855.1482544E-511.210953
TGTAGGA10550.011.2003232
Produced by FastQC (version 0.11.5)