##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926951_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 680521 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.383416529394392 30.0 26.0 31.0 10.0 31.0 2 27.02010371465392 31.0 27.0 31.0 10.0 34.0 3 26.633203089985468 31.0 27.0 31.0 10.0 34.0 4 30.34108719642744 35.0 30.0 37.0 10.0 37.0 5 29.907485588247827 35.0 30.0 37.0 10.0 37.0 6 29.68945557888735 35.0 30.0 37.0 8.0 37.0 7 29.72130176732239 35.0 30.0 37.0 8.0 37.0 8 29.44662398368309 35.0 29.0 37.0 7.0 37.0 9 31.638337391498574 37.0 32.0 39.0 7.0 39.0 10 31.820386145321013 37.0 32.0 39.0 2.0 39.0 11 32.04171509769721 37.0 32.0 39.0 2.0 39.0 12 31.889281888435477 37.0 32.0 39.0 2.0 39.0 13 32.01536616798012 37.0 32.0 39.0 2.0 39.0 14 32.91466538137691 38.0 32.0 40.0 2.0 41.0 15 32.501129281829655 38.0 32.0 40.0 2.0 41.0 16 32.570778859138805 38.0 32.0 40.0 2.0 41.0 17 32.79260302033295 38.0 32.0 40.0 2.0 41.0 18 32.83189350512328 38.0 32.0 40.0 2.0 41.0 19 32.1874269860886 38.0 31.0 40.0 2.0 41.0 20 32.46641911123977 38.0 32.0 40.0 2.0 41.0 21 32.45446944326479 38.0 32.0 40.0 2.0 41.0 22 32.476355615770856 39.0 32.0 40.0 2.0 41.0 23 32.443116377011144 39.0 32.0 40.0 2.0 41.0 24 31.150174645602412 38.0 30.0 40.0 2.0 41.0 25 31.67183525563502 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.7985115079003684 1101 2 -0.7304038179258328 1101 3 -1.8610418752249984 1101 4 -0.8255141930190959 1101 5 -1.2021449666802724 1101 6 -2.508260724219131 1101 7 -1.2497242294891144 1101 8 -0.4849331120727136 1101 9 -0.6614992281042333 1101 10 -9.268525584405122 1101 11 -4.574973404015704 1101 12 -2.3981671970435308 1101 13 -1.5655815818312497 1101 14 -1.4832571196486803 1101 15 -2.4606834266740947 1101 16 -0.8748766145338962 1101 17 -0.7390865022062201 1101 18 -0.4500934323962298 1101 19 -1.3218042990016095 1101 20 -0.5413253436966272 1101 21 -0.4460794609963443 1101 22 -0.3255062904242578 1101 23 -0.47219431713194027 1101 24 -0.6094169465186994 1101 25 -0.6150537102468405 1102 1 -1.0450388233932024 1102 2 -0.4834484882118808 1102 3 -0.9434967438867616 1102 4 -0.9130963564633277 1102 5 -0.9725281577531177 1102 6 -1.6041063235130082 1102 7 -0.6900473111915097 1102 8 -0.09647174430099881 1102 9 0.06553125065038401 1102 10 -0.19930858415364128 1102 11 -0.39511867243683696 1102 12 -0.3486457544186372 1102 13 -0.5263137425634135 1102 14 -0.7563518892951748 1102 15 -0.3099218276366358 1102 16 -0.24555961280309901 1102 17 -0.6327387751688391 1102 18 -0.7072895447647554 1102 19 -0.15071202446105758 1102 20 -0.411936841894331 1102 21 -0.6845346649687905 1102 22 -0.6461471283064775 1102 23 -1.1955906198385868 1102 24 -5.00454606578575 1102 25 -3.386640074936654 1103 1 -0.9189127856721662 1103 2 -0.7865356469660263 1103 3 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6 1.843779151076557 2215 7 1.3540647115091282 2215 8 0.2209203263388666 2215 9 0.5563214548327267 2215 10 0.5319761695850005 2215 11 0.35133215019682495 2215 12 0.028903881190338154 2215 13 0.41283780356487654 2215 14 0.400030461313321 2215 15 0.7108136517997252 2215 16 0.9492543142481935 2215 17 0.29860414013558767 2215 18 -0.28553800039893673 2215 19 0.6905157133184048 2215 20 -2.862535641651114 2215 21 -1.4856485470770586 2215 22 -0.7577292110192744 2215 23 -0.8902635491546604 2215 24 -0.3687344035105724 2215 25 -0.4291782104178523 2216 1 0.28852328124257554 2216 2 -0.6524541909386237 2216 3 0.04434105123950616 2216 4 -0.1760196021862832 2216 5 0.10683862736290095 2216 6 -1.2918343987185636 2216 7 -8.437245389512569 2216 8 -9.846383471689894 2216 9 -5.388692966297054 2216 10 -2.9498109027206887 2216 11 -1.9698614176021607 2216 12 -1.853236381718581 2216 13 -1.6160610255279266 2216 14 -1.6421474490248045 2216 15 -2.534576751713157 2216 16 -1.8241421036710612 2216 17 -2.134208480279618 2216 18 -1.7670602617370292 2216 19 -0.9860689302689885 2216 20 -1.9275364860947555 2216 21 -4.591069547324651 2216 22 -3.0084486278120615 2216 23 -2.878859460735672 2216 24 -3.310903951397087 2216 25 -2.4444954342806824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58007.0 3 6.0 4 472.0 5 2252.0 6 2703.0 7 2226.0 8 1733.0 9 1604.0 10 1583.0 11 1485.0 12 1511.0 13 1537.0 14 1584.0 15 1578.0 16 1696.0 17 1797.0 18 2064.0 19 2615.0 20 3330.0 21 4366.0 22 5384.0 23 6434.0 24 7344.0 25 8273.0 26 9396.0 27 10274.0 28 11433.0 29 13356.0 30 15882.0 31 19021.0 32 24522.0 33 31239.0 34 42020.0 35 57301.0 36 80665.0 37 121205.0 38 122623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96521337036525 18.550954710523758 12.54951741687701 25.934314502233992 2 13.707848967659258 19.72224715265813 42.9790088999204 23.590894979762208 3 19.874083915610623 23.959367859470703 28.848142965810798 27.318405259107877 4 11.309029253422311 16.52017652344112 38.67869175013198 33.492102473004586 5 12.154074091494353 37.92617895547573 36.28496004985684 13.634786903173083 6 31.23412623756555 37.304664534487216 19.269424781882748 12.191784446064483 7 27.567599428259875 30.64954393410253 23.423072955808024 18.35978368182957 8 24.771654680287085 38.33062958329834 20.810182013210802 16.087533723203777 9 26.078106007939645 15.53624943597944 20.438194369935754 37.947450186145154 10 14.087233917440903 29.721274844172648 35.40926731741738 20.78222392096907 11 34.24156824474871 22.61671007225119 24.779248799242804 18.3624728837573 12 23.299632420483924 24.98497200898887 32.390060508791194 19.325335061736016 13 29.201908846270857 22.530059037846243 25.491945309526155 22.776086806356748 14 19.658021675676153 23.29914725879417 27.91573705823391 29.127094007295774 15 22.524161689319474 32.93881978083996 22.70184710530029 21.83517142454028 16 20.758962691217796 28.756599292156103 29.70210589438781 20.78233212223829 17 20.75154729027621 29.691658962672495 28.70750738922048 20.84928635783081 18 20.601661336594084 28.196279922870715 31.175927667779714 20.02613107275549 19 23.537668736183623 27.239869949673114 27.34261088377781 21.879850430365458 20 22.92129018603817 28.242440453969586 28.707903315412448 20.12836604457979 21 21.92300852570424 27.59074268128328 27.301800524093455 23.184448268919024 22 22.0873665119533 29.626483322996243 27.677623978906464 20.60852618614399 23 21.011772659798055 29.118005849586265 28.434280339217217 21.435941151398463 24 23.243509380442532 27.50391311057385 28.882650774171264 20.369926734812356 25 21.620167810338163 28.363885945406526 28.743803377664385 21.27214286659093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 249.0 1 249.0 2 295.5 3 342.0 4 342.0 5 342.0 6 491.0 7 640.0 8 640.0 9 640.0 10 978.5 11 1317.0 12 1317.0 13 1317.0 14 2186.5 15 3056.0 16 3056.0 17 3056.0 18 5440.0 19 7824.0 20 7824.0 21 7824.0 22 12472.0 23 17120.0 24 17120.0 25 17120.0 26 25354.5 27 33589.0 28 33589.0 29 33589.0 30 44838.0 31 56087.0 32 56087.0 33 56087.0 34 64028.0 35 71969.0 36 71969.0 37 71969.0 38 77627.0 39 83285.0 40 83285.0 41 83285.0 42 86438.5 43 89592.0 44 89592.0 45 89592.0 46 90775.5 47 91959.0 48 91959.0 49 91959.0 50 86000.0 51 80041.0 52 80041.0 53 80041.0 54 68790.0 55 57539.0 56 57539.0 57 57539.0 58 48380.5 59 39222.0 60 39222.0 61 39222.0 62 31637.5 63 24053.0 64 24053.0 65 24053.0 66 18362.5 67 12672.0 68 12672.0 69 12672.0 70 9259.5 71 5847.0 72 5847.0 73 5847.0 74 4190.5 75 2534.0 76 2534.0 77 2534.0 78 1760.0 79 986.0 80 986.0 81 986.0 82 695.0 83 404.0 84 404.0 85 404.0 86 263.5 87 123.0 88 123.0 89 123.0 90 83.5 91 44.0 92 44.0 93 44.0 94 31.5 95 19.0 96 19.0 97 19.0 98 13.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21557012935677225 2 0.1251981937368575 3 0.12666765610466096 4 0.07317922591661388 5 0.025421698963000408 6 0.022482774227393424 7 0.2778753337516403 8 0.2694993982551604 9 0.020278580675688187 10 0.04129189253527812 11 0.017192709703300852 12 0.018221333360763297 13 0.01616408604583841 14 0.018662172071104346 15 0.015429354861936664 16 0.02130720433315063 17 0.020278580675688187 18 0.015429354861936664 19 0.02483391401587901 20 0.01807438712398295 21 0.015870193572277713 22 0.01807438712398295 23 0.019249957018225742 24 0.017045763466520505 25 0.01616408604583841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 680521.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.83800578835344 #Duplication Level Percentage of deduplicated Percentage of total 1 94.42663874710328 81.05394363348611 2 4.203375883449921 7.216188068283991 3 0.656077592982575 1.6894917667214178 4 0.2322017211818701 0.7972693074749999 5 0.11636352268286539 0.49942063668024983 6 0.07457939215644725 0.38410477773702 7 0.04243987013523874 0.2550067672827914 8 0.03682031164711868 0.25284656994354804 9 0.02871440813472728 0.22183087785100772 >10 0.15150949966111846 2.459623715712918 >50 0.015554289478969911 0.9356257909298135 >100 0.013673705556288735 2.3718019962831574 >500 0.0010255279147813312 0.5843676445533746 >1k 0.0010255279147813312 1.2784784470595298 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1952 0.2868390541952416 No Hit CTGTAGGACGTGGAATATGGCAAGA 1642 0.24128572079333332 No Hit GTCCTACAGTGTGCATTTCTCATTT 1581 0.23232200034973205 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1407 0.2067533551499513 No Hit CTTTAGGACGTGAAATATGGCGAGG 1083 0.15914277443311817 No Hit CTGTAGGACCTGGAATATGGCGAGA 1049 0.15414660238258626 No Hit GTATCAACGCAGAGTACTTTTTTTT 842 0.123728731369054 No Hit GTACATGGGAAGCAGTGGTATCAAC 788 0.11579363458291515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.4694623678034916E-4 0.0 0.0 0.0 0.0 9 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 0.0 10 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 0.0 11 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 0.0 12 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 0.0 13 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGTT 25 0.005978114 19.027714 6 GTACCGT 40 0.0052242847 14.270784 6 CGGTTTC 40 0.0053299316 14.227792 13 CCCCTAT 50 0.001453905 13.3528595 1 TAGGACC 405 0.0 12.689818 4 AATGCCG 90 5.4880184E-7 12.647855 18 TCCAACG 60 4.1383313E-4 12.647855 18 TATGACT 70 1.07074404E-4 12.236609 4 ACTGTTC 250 0.0 12.168767 8 GATATAC 230 0.0 12.025867 1 GGACCTG 580 0.0 11.974336 6 AGGACCT 610 0.0 11.85333 5 GGCGAGG 340 0.0 11.717865 19 GTAGGAC 985 0.0 11.706053 3 TAATTCC 75 2.03571E-4 11.420836 4 TACACTG 425 0.0 11.416628 5 GTCCTAC 1050 0.0 11.263637 1 TATACAC 330 0.0 11.261909 3 GTATGAC 85 5.1482544E-5 11.21095 3 TGTAGGA 1055 0.0 11.200323 2 >>END_MODULE