FastQCFastQC Report
Thu 26 May 2016
SRR1926951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680521
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA20360.29918253808479095No Hit
GTCCTACAGTGGACATTTCTAAATT20210.2969783445330857No Hit
GTCCTACAGTGTGCATTTCTCATTT16730.24584105413352417No Hit
GTCCTAAAGTGTGTATTTCTCATTT14430.21204341967404386No Hit
CTTTAGGACGTGAAATATGGCGAGG13840.20337359170400326No Hit
GTATCAACGCAGAGTACTTTTTTTT13410.19705490352244825No Hit
CTGTAGGACCTGGAATATGGCGAGA13260.194850709970743No Hit
GGTATCAACGCAGAGTACTTTTTTT9250.13592526902182298No Hit
GTACATGGGAAGCAGTGGTATCAAC8570.12593292492075925No Hit
CTGAAGGACCTGGAATATGGCGAGA7860.11549974210935444No Hit
CCCATGTACTCTGCGTTGATACCAC7690.11300165608408852No Hit
TATCAACGCAGAGTACTTTTTTTTT6810.10007038724741779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG351.00094214E-418.9800859
GGCGTGC508.786161E-515.1829538
TAGGACC5050.014.8895774
CGTGCGC456.8093423E-414.76228810
CTACACT1400.014.2770314
TATGACG400.00531189414.23506416
GAGTAAG759.320702E-713.9762451
TCCAACG759.764499E-713.91872918
CGAAATC759.772593E-713.91770613
CCTACAC1301.8189894E-1213.9140293
TGTAGGA12750.013.4530692
CTGTAGG12200.013.3565831
AAGGCGT655.3477233E-513.1787986
GTAGGAC12400.013.05178453
ACGAAAT802.019984E-613.0478512
AGGACCT7700.012.7318985
TCAATAC604.0144337E-412.69350053
TTACCCT604.021932E-412.6906944
CCAACGA751.49168845E-512.6533919
GGACCTG7850.012.3673636
Produced by FastQC (version 0.11.5)