##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926951_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 680521 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.679361841882912 31.0 30.0 33.0 25.0 34.0 2 30.06659897343359 31.0 30.0 33.0 26.0 34.0 3 30.150331878075768 31.0 30.0 34.0 26.0 34.0 4 33.73993455014614 35.0 35.0 37.0 28.0 37.0 5 33.688970656305976 37.0 35.0 37.0 28.0 37.0 6 33.63235961858635 37.0 35.0 37.0 29.0 37.0 7 33.62437162115497 37.0 35.0 37.0 28.0 37.0 8 33.648476681836414 37.0 35.0 37.0 29.0 37.0 9 35.178335422418996 39.0 35.0 39.0 29.0 39.0 10 35.06140883235051 39.0 35.0 39.0 28.0 39.0 11 35.10510182639478 39.0 35.0 39.0 29.0 39.0 12 34.98610329438768 39.0 35.0 39.0 28.0 39.0 13 35.00695055699971 39.0 35.0 39.0 28.0 39.0 14 36.07094123473045 40.0 36.0 41.0 28.0 41.0 15 36.07628566936215 40.0 36.0 41.0 28.0 41.0 16 36.05187349104583 40.0 36.0 41.0 27.0 41.0 17 36.02984771961482 40.0 36.0 41.0 27.0 41.0 18 35.99852612924509 40.0 36.0 41.0 27.0 41.0 19 36.063570411493544 40.0 36.0 41.0 27.0 41.0 20 36.03358750134088 40.0 36.0 41.0 27.0 41.0 21 35.96662116231534 40.0 36.0 41.0 27.0 41.0 22 35.88771250262666 40.0 36.0 41.0 27.0 41.0 23 35.81606739542204 40.0 36.0 41.0 27.0 41.0 24 35.7421681329452 40.0 36.0 41.0 27.0 41.0 25 35.62565152287733 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19771734846826305 1101 2 0.25722086322090476 1101 3 0.5611285375284041 1101 4 0.5719328237764003 1101 5 0.4458918974760593 1101 6 0.5216634560232691 1101 7 0.49754898672760817 1101 8 0.5607560926440414 1101 9 0.6734323527392831 1101 10 0.5974847503736953 1101 11 0.7287967418966872 1101 12 0.713331595739831 1101 13 0.7917393049504753 1101 14 0.7958078484815019 1101 15 0.7145581372074474 1101 16 0.5709414404792312 1101 17 0.6667797692605149 1101 18 0.8618429090388631 1101 19 0.616910873785379 1101 20 0.742278280506099 1101 21 0.8147979130475989 1101 22 0.6416609454713935 1101 23 0.5953050431088442 1101 24 0.708058486180235 1101 25 0.6906740733801016 1102 1 -1.0423749605895658 1102 2 -0.6599071021829239 1102 3 -0.3877849079367657 1102 4 -0.5163870231641425 1102 5 -0.7145907526617634 1102 6 -0.6567425361758268 1102 7 -0.5146770323949568 1102 8 -0.4757552955914903 1102 9 -0.4146833491695219 1102 10 -0.43743391213117633 1102 11 -0.48611274887487355 1102 12 -0.5052170021191102 1102 13 -0.2739441124570803 1102 14 -0.3575060565565451 1102 15 -0.5704825685229125 1102 16 -0.4491742417054141 1102 17 -0.432525447286082 1102 18 -0.3396837407636468 1102 19 -0.3528253649163773 1102 20 -0.35802103358631854 1102 21 -0.5447409361464182 1102 22 -0.5929085994429784 1102 23 -0.511948022764912 1102 24 -0.2925628247859038 1102 25 -0.6413969138633036 1103 1 -0.6969831289015396 1103 2 -0.44051472096886357 1103 3 -0.38656417510454943 1103 4 -0.36377579320711817 1103 5 -0.6084812007217408 1103 6 -0.6961160764973044 1103 7 -0.663248264707093 1103 8 -0.6105147798220543 1103 9 -0.4283596783356174 1103 10 -0.4466021652271692 1103 11 -0.26333881995999064 1103 12 -0.2860542442834628 1103 13 -0.20921583345486283 1103 14 -0.4644055521701489 1103 15 -0.3410682062675008 1103 16 -0.48975870189421755 1103 17 -0.4501259811113698 1103 18 -0.5816174836247114 1103 19 -0.2886799760285754 1103 20 -0.37892559268980364 1103 21 -0.44788010361448016 1103 22 -0.555079619795066 1103 23 -0.7222565423810252 1103 24 -0.4334475617003193 1103 25 -0.5416628452117962 1104 1 -0.4714327400366649 1104 2 -0.37555267277843996 1104 3 -0.2538477290607659 1104 4 -0.1846331263379426 1104 5 -0.31640999385808755 1104 6 -0.1969319722495797 1104 7 -0.2552507338140444 1104 8 -0.2462708756732539 1104 9 -0.12632928573203372 1104 10 -0.23600856699059136 1104 11 -0.08713649339138385 1104 12 0.012216991399419896 1104 13 0.07423881325643578 1104 14 -0.08339099845928644 1104 15 -0.03566129043132804 1104 16 0.06935187437353818 1104 17 0.008722885397176583 1104 18 -0.2508885907894083 1104 19 -0.1987522967164992 1104 20 -0.23495114833623632 1104 21 -0.24766337522014226 1104 22 -0.1453694867203268 1104 23 -0.22071919227023074 1104 24 -0.08465504846878247 1104 25 -0.22142570968742348 1105 1 -0.3305688628150669 1105 2 -0.4899672470795977 1105 3 -0.4573741149800128 1105 4 -0.49357360484242463 1105 5 -0.40229346367760144 1105 6 -0.5393024089771785 1105 7 -0.6049483232624908 1105 8 -0.7831728507085387 1105 9 -0.7341308300400371 1105 10 -0.4451775084268945 1105 11 -0.40614511638811024 1105 12 -0.5416351866044735 1105 13 -0.39678639654352565 1105 14 -0.35475481793131536 1105 15 -0.5585737843394583 1105 16 -0.47682101938697485 1105 17 -0.5477604193430778 1105 18 -0.29528251609808365 1105 19 -0.4945117880695591 1105 20 -0.34896570122779735 1105 21 -0.4285462957144759 1105 22 -0.4277254437997229 1105 23 -0.5174590177820164 1105 24 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0.3889581722196098 1107 11 0.3788450710281097 1107 12 0.5237609362551296 1107 13 0.49946995823986384 1107 14 0.3747564245647439 1107 15 0.26475016304417665 1107 16 0.3319230524637149 1107 17 0.27577271636234 1107 18 0.5957028434718623 1107 19 0.4183943056413071 1107 20 0.3264125580501158 1107 21 0.5427866467669915 1107 22 0.35965114197161796 1107 23 0.3731602622248076 1107 24 0.543882849225227 1107 25 0.41354307418606595 1108 1 0.6324835910339459 1108 2 0.40224053470296894 1108 3 0.5784522679440549 1108 4 0.7124689030311373 1108 5 0.708460873136957 1108 6 0.7007255264502419 1108 7 0.5440941391223433 1108 8 0.35285180953083994 1108 9 0.3786250105781832 1108 10 0.4815080494387729 1108 11 0.3100186323107792 1108 12 0.4814088652098576 1108 13 0.34256589755979405 1108 14 0.36649831610790073 1108 15 0.3115478846150239 1108 16 0.2172646439055015 1108 17 0.3672211577241882 1108 18 0.24526532867298556 1108 19 0.36702589130986496 1108 20 0.32580330971357796 1108 21 0.24453963248291188 1108 22 0.46670325797889234 1108 23 0.3087901159253974 1108 24 0.4189922611808612 1108 25 0.3891385545743873 1109 1 0.2833709265746691 1109 2 0.37361367112314525 1109 3 0.6606582559260445 1109 4 0.47032177876912584 1109 5 0.4409813265277549 1109 6 0.3556842692893625 1109 7 0.3038005212810333 1109 8 0.22805546749058436 1109 9 0.6109510034654804 1109 10 0.42785096564556824 1109 11 0.4626929022079409 1109 12 0.31778699453484904 1109 13 0.2895701546681124 1109 14 0.548730582536308 1109 15 0.5306374240414513 1109 16 0.4738622858529098 1109 17 0.4826711323216841 1109 18 0.24141115653164036 1109 19 0.37709841982198355 1109 20 0.3564273352211913 1109 21 0.4916936767358209 1109 22 0.4880422324187208 1109 23 0.40818395976134525 1109 24 0.032323317048835065 1109 25 0.26383591446646903 1110 1 0.36984656723927145 1110 2 0.033072274333509455 1110 3 0.43066318411528925 1110 4 0.458881339456255 1110 5 0.3958707942833115 1110 6 0.5398542214155242 1110 7 0.5316623911768374 1110 8 0.5188186327961333 1110 9 0.5145626621531747 1110 10 0.46348881413894816 1110 11 0.38931920465795855 1110 12 0.5170653668243474 1110 13 0.47849228927808696 1110 14 0.34047938569035097 1110 15 0.4755719495272359 1110 16 0.26966628472646903 1110 17 0.5378140498097181 1110 18 0.5224587487591847 1110 19 0.501320469948709 1110 20 0.5903614382907492 1110 21 0.27987306282424385 1110 22 0.3563046936972327 1110 23 0.5600953931603883 1110 24 0.5007336240046243 1110 25 0.5465626111716233 1111 1 0.46174996844883864 1111 2 0.28462931256243706 1111 3 0.26498946164954873 1111 4 0.5316425334861066 1111 5 0.2840934819601699 1111 6 0.5132805377890435 1111 7 0.08232335217112308 1111 8 0.07774334011455863 1111 9 0.1988341485871743 1111 10 0.40301053638275164 1111 11 0.2806525010015193 1111 12 0.2810787108847137 1111 13 0.3651891488464116 1111 14 0.4598268861064838 1111 15 0.6957177354674187 1111 16 0.33644207122149794 1111 17 0.32388948120294003 1111 18 0.4322823075451794 1111 19 0.43988161404236337 1111 20 0.45621340522189513 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2213 1 0.03947422470789519 2213 2 0.012039342177313728 2213 3 0.05099240670261551 2213 4 -0.17989592805235333 2213 5 -0.027663886424555528 2213 6 -0.07177348224126234 2213 7 -0.20327119685119044 2213 8 -0.3731406688389285 2213 9 -0.2875392289219505 2213 10 -0.24968602783156513 2213 11 -0.30245732384013735 2213 12 -0.15609485989352123 2213 13 -0.2087173851613926 2213 14 -0.27343380696705566 2213 15 -0.31162188897257437 2213 16 -0.2749415778175788 2213 17 -0.2696102016122168 2213 18 -0.22461490155687613 2213 19 -0.1424426441888258 2213 20 -0.34824460312023575 2213 21 -0.376571174873213 2213 22 -0.39346436551598174 2213 23 -0.3917509784299327 2213 24 -0.5808033199229499 2213 25 -0.5289805945504327 2214 1 0.6450529819741071 2214 2 0.6558162671354566 2214 3 0.595814819555109 2214 4 0.6770326044080903 2214 5 0.5068517935423387 2214 6 0.69029219114136 2214 7 0.6727587709799465 2214 8 0.5920826319493031 2214 9 0.6811102433958993 2214 10 0.6691228778628826 2214 11 0.5675503891609708 2214 12 0.6332861247369124 2214 13 0.6621815557118751 2214 14 0.7188931678434116 2214 15 0.5449656724022205 2214 16 0.6702961143019905 2214 17 0.6986653782737804 2214 18 0.6497478352416621 2214 19 0.7198356070292604 2214 20 0.7926611101442447 2214 21 0.7312862098550497 2214 22 0.7762263619517071 2214 23 0.9230876129126955 2214 24 0.7448852065175942 2214 25 0.8611796853750704 2215 1 0.35796167150328273 2215 2 0.20415169387266374 2215 3 0.22400250272794864 2215 4 0.08307366049862708 2215 5 0.3080957829357587 2215 6 0.15897518635825492 2215 7 -0.20860707989357508 2215 8 -0.17873563521978042 2215 9 -0.2782142849538687 2215 10 -0.361965755588443 2215 11 -0.42118329180427594 2215 12 -0.2585150272695813 2215 13 -0.35157305929212157 2215 14 -0.1614830739257016 2215 15 -0.16433506726714597 2215 16 -0.012280421812398856 2215 17 -0.20735823278446475 2215 18 -0.421497676627304 2215 19 -0.41392497100395076 2215 20 -0.13678324971822065 2215 21 0.023434993777705415 2215 22 -0.2243096763131831 2215 23 -0.16641016046682466 2215 24 -0.11064930927174998 2215 25 -0.016067551966180815 2216 1 -0.4262411654994196 2216 2 -0.3615583192613059 2216 3 -2.306970542303034 2216 4 -2.060245642499229 2216 5 -1.0507780079931166 2216 6 -0.6176325769482958 2216 7 -0.590539101360477 2216 8 -0.6166862442850061 2216 9 -0.5577915384296119 2216 10 -0.7178626777143435 2216 11 -0.667642777365927 2216 12 -0.5600453559139353 2216 13 -0.6719131651181272 2216 14 -0.8827240289504701 2216 15 -0.8077322558437103 2216 16 -0.7610018685493145 2216 17 -0.8328261611428545 2216 18 -0.7476070542149458 2216 19 -0.8167491840726626 2216 20 -0.7608123444910362 2216 21 -0.6770523239905373 2216 22 -0.7483541842320918 2216 23 -1.0038752554298398 2216 24 -0.950168943816216 2216 25 -0.9301318838144894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17244.0 3 17.0 4 293.0 5 1886.0 6 2632.0 7 1919.0 8 1509.0 9 1294.0 10 1115.0 11 1035.0 12 942.0 13 919.0 14 918.0 15 875.0 16 919.0 17 1041.0 18 1095.0 19 1365.0 20 1654.0 21 2252.0 22 2760.0 23 3266.0 24 3889.0 25 4802.0 26 5705.0 27 6114.0 28 7068.0 29 8835.0 30 11005.0 31 14014.0 32 18322.0 33 24368.0 34 32991.0 35 45599.0 36 66901.0 37 115948.0 38 268010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48623799226209 19.499977933712874 10.278770760698471 28.73501331332657 2 13.563308679458439 20.62040681835566 41.30127152801069 24.515012974175214 3 18.802634943870757 24.431726323277395 29.059339147611528 27.70629958524032 4 11.702504404713448 17.319524305642293 38.354437262038935 32.623534027605324 5 12.381113331765812 39.00864095932283 35.886873971314365 12.723371737596988 6 31.72818482724049 38.364331999026916 17.654422009421236 12.253061164311358 7 28.4519172827349 31.45607666191449 21.552932876732463 18.539073178618146 8 24.470094099354693 39.68823618453056 19.465472164784828 16.376197551329927 9 26.07903968444087 15.675082262584816 19.86632238115463 38.37955567181968 10 14.245260005349117 30.254406409536717 34.519593349459065 20.980740235655105 11 34.25846053464966 22.712396929827783 24.75535759159303 18.273784943929535 12 23.520793749458132 25.085927317025142 32.08237266666373 19.310906266852996 13 29.438543693315072 22.526729716861908 25.42555942843372 22.609167161389298 14 19.803209599703756 23.539464616080917 27.474831783295446 29.182494000919885 15 22.1944484855966 33.09336237084845 22.66322213552114 22.04896700803381 16 20.921771126615685 28.999759006401028 29.250451128261496 20.82801873872179 17 20.523306476380526 30.102318965377894 28.430359769528845 20.944014788712735 18 20.4782841255753 28.358480917421485 31.13519740902492 20.0280375479783 19 23.008434726385705 27.40933403867631 27.639893022982427 21.942338211955565 20 22.98858513245831 28.23386959307349 28.687789853581698 20.089755420886505 21 21.665694269519097 27.417190707900225 27.472448339258342 23.444666683322335 22 22.14891237742847 29.79893346421345 27.327738600278316 20.724415558079766 23 20.96382178334215 29.1356609651443 28.41988479398107 21.480632457532476 24 22.61600721803097 27.48171246021413 29.280489274346895 20.621791047408003 25 21.570327482200458 28.569056348687354 28.616521796633332 21.24409437247886 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 230.0 1 230.0 2 245.5 3 261.0 4 261.0 5 261.0 6 412.0 7 563.0 8 563.0 9 563.0 10 898.0 11 1233.0 12 1233.0 13 1233.0 14 2001.5 15 2770.0 16 2770.0 17 2770.0 18 5080.5 19 7391.0 20 7391.0 21 7391.0 22 12127.0 23 16863.0 24 16863.0 25 16863.0 26 24759.0 27 32655.0 28 32655.0 29 32655.0 30 44136.5 31 55618.0 32 55618.0 33 55618.0 34 63765.0 35 71912.0 36 71912.0 37 71912.0 38 77155.0 39 82398.0 40 82398.0 41 82398.0 42 85729.5 43 89061.0 44 89061.0 45 89061.0 46 91148.0 47 93235.0 48 93235.0 49 93235.0 50 87786.5 51 82338.0 52 82338.0 53 82338.0 54 70530.5 55 58723.0 56 58723.0 57 58723.0 58 49211.0 59 39699.0 60 39699.0 61 39699.0 62 31814.0 63 23929.0 64 23929.0 65 23929.0 66 18127.5 67 12326.0 68 12326.0 69 12326.0 70 9013.0 71 5700.0 72 5700.0 73 5700.0 74 3981.0 75 2262.0 76 2262.0 77 2262.0 78 1592.0 79 922.0 80 922.0 81 922.0 82 621.0 83 320.0 84 320.0 85 320.0 86 205.5 87 91.0 88 91.0 89 91.0 90 53.5 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11035662382204224 2 0.10418488187726757 3 0.018662172071104346 4 0.0 5 0.006024795707994315 6 0.3329801725442712 7 0.019249957018225742 8 0.010286236574624442 9 0.011461806468867235 10 0.005143118287312221 11 0.005583956997653268 12 5.877849471213967E-4 13 7.347311839017458E-4 14 0.0 15 0.0032328172091676817 16 7.347311839017458E-4 17 2.9389247356069833E-4 18 7.347311839017458E-4 19 1.4694623678034916E-4 20 7.347311839017458E-4 21 0.01058012904818514 22 0.0 23 1.4694623678034916E-4 24 4.408387103410475E-4 25 0.0038206021562890782 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 680521.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.3785840717188 #Duplication Level Percentage of deduplicated Percentage of total 1 92.40267147164725 76.12001240278505 2 5.788658396342471 9.537229647311184 3 0.8968201398134078 2.216363198544883 4 0.3156075632340166 1.0399721672617501 5 0.1583261297569186 0.6521341195465089 6 0.08983138143311085 0.44401092045996976 7 0.058774418818299624 0.3389227381322795 8 0.04083887031973272 0.2691398649622499 9 0.033482044797064264 0.24823830979872125 >10 0.17695764913089237 2.773263766167099 >50 0.019462540250493245 1.1274510493825838 >100 0.015891116411602325 2.7584977686035717 >500 0.001428414957218394 0.8238426999996656 >1k 0.0012498630875660947 1.6509213470445008 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2036 0.29918253808479095 No Hit GTCCTACAGTGGACATTTCTAAATT 2021 0.2969783445330857 No Hit GTCCTACAGTGTGCATTTCTCATTT 1673 0.24584105413352417 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1443 0.21204341967404386 No Hit CTTTAGGACGTGAAATATGGCGAGG 1384 0.20337359170400326 No Hit GTATCAACGCAGAGTACTTTTTTTT 1341 0.19705490352244825 No Hit CTGTAGGACCTGGAATATGGCGAGA 1326 0.194850709970743 No Hit GGTATCAACGCAGAGTACTTTTTTT 925 0.13592526902182298 No Hit GTACATGGGAAGCAGTGGTATCAAC 857 0.12593292492075925 No Hit CTGAAGGACCTGGAATATGGCGAGA 786 0.11549974210935444 No Hit CCCATGTACTCTGCGTTGATACCAC 769 0.11300165608408852 No Hit TATCAACGCAGAGTACTTTTTTTTT 681 0.10007038724741779 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.4694623678034916E-4 0.0 0.0 0.0 6 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 7 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 8 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 9 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 10 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 11 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 12 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 13 0.0 1.4694623678034916E-4 0.0 0.0 1.4694623678034916E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGCG 35 1.00094214E-4 18.980085 9 GGCGTGC 50 8.786161E-5 15.182953 8 TAGGACC 505 0.0 14.889577 4 CGTGCGC 45 6.8093423E-4 14.762288 10 CTACACT 140 0.0 14.277031 4 TATGACG 40 0.005311894 14.235064 16 GAGTAAG 75 9.320702E-7 13.976245 1 TCCAACG 75 9.764499E-7 13.918729 18 CGAAATC 75 9.772593E-7 13.917706 13 CCTACAC 130 1.8189894E-12 13.914029 3 TGTAGGA 1275 0.0 13.453069 2 CTGTAGG 1220 0.0 13.356583 1 AAGGCGT 65 5.3477233E-5 13.178798 6 GTAGGAC 1240 0.0 13.0517845 3 ACGAAAT 80 2.019984E-6 13.04785 12 AGGACCT 770 0.0 12.731898 5 TCAATAC 60 4.0144337E-4 12.6935005 3 TTACCCT 60 4.021932E-4 12.690694 4 CCAACGA 75 1.49168845E-5 12.65339 19 GGACCTG 785 0.0 12.367363 6 >>END_MODULE