FastQCFastQC Report
Thu 26 May 2016
SRR1926950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696558
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17690.25396305835264255No Hit
GTCCTACAGTGTGCATTTCTCATTT15010.21548815748293754No Hit
CTGTAGGACGTGGAATATGGCAAGA14640.21017632415391108No Hit
GTCCTAAAGTGTGTATTTCTCATTT12200.1751469367949259No Hit
GTATCAACGCAGAGTACTTTTTTTT10450.1500234007792603No Hit
CTTTAGGACGTGAAATATGGCGAGG10020.1438501890725539No Hit
CTGTAGGACCTGGAATATGGCGAGA9410.1350928422328076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT508.3701656E-515.2696061
TAGGACC3900.013.4287044
CGCCTTG655.272393E-513.1988212
CTGGACT801.9433173E-613.0929864
GTCCTAT1102.5538611E-913.0138691
AGGACCT5200.012.4448935
CTAGACA550.003012441812.1191284
CCAACGA550.003106432512.06952419
GTCTAGT802.7302765E-511.92937851
GTCCTAC9700.011.8063961
GACGTGG3700.011.5743447
TGTAGGA9600.011.51841452
GTAGGAC9500.011.4389783
GGACTTG1251.7771526E-811.4200186
GGACCTG5000.011.4200186
GGACGTG6900.011.3096816
ATAGGAC1104.738613E-711.265663
GTCCTAG1451.1223165E-911.1889341
CTGTAGG9500.011.150831
TCCAACG600.005947995511.06372918
Produced by FastQC (version 0.11.5)