##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926950_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 696558 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.381617898294184 30.0 26.0 31.0 10.0 31.0 2 27.028393902589592 31.0 28.0 31.0 10.0 34.0 3 26.64758139307854 31.0 27.0 31.0 10.0 34.0 4 30.32427163280014 35.0 30.0 37.0 10.0 37.0 5 29.872560504652878 35.0 30.0 37.0 10.0 37.0 6 29.702732579340125 35.0 30.0 37.0 8.0 37.0 7 29.723951200043643 35.0 30.0 37.0 7.0 37.0 8 29.45362913066823 35.0 29.0 37.0 2.0 37.0 9 31.637056784933918 37.0 32.0 39.0 2.0 39.0 10 31.83331036324326 37.0 32.0 39.0 2.0 39.0 11 32.03738382159131 37.0 32.0 39.0 2.0 39.0 12 31.910252986829526 37.0 32.0 39.0 2.0 39.0 13 32.017236181337374 37.0 32.0 39.0 2.0 39.0 14 32.92270421127889 38.0 32.0 40.0 2.0 41.0 15 32.51249285773762 38.0 32.0 40.0 2.0 41.0 16 32.575426597641545 38.0 32.0 40.0 2.0 41.0 17 32.79240924660976 38.0 32.0 40.0 2.0 41.0 18 32.828957531174716 38.0 32.0 40.0 2.0 41.0 19 32.234022148909354 38.0 31.0 40.0 2.0 41.0 20 32.513250870709975 39.0 32.0 40.0 2.0 41.0 21 32.493236744104586 39.0 32.0 40.0 2.0 41.0 22 32.495204993697584 39.0 32.0 40.0 2.0 41.0 23 32.46519026412732 39.0 32.0 40.0 2.0 41.0 24 31.203749005825788 38.0 30.0 40.0 2.0 41.0 25 31.730385696524912 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.032421127831185 1101 2 -0.99702332662347 1101 3 -1.662484869896712 1101 4 -1.052999139821786 1101 5 -1.37157140081624 1101 6 -2.8208549832262264 1101 7 -1.5064509926549228 1101 8 -1.040281388135238 1101 9 -1.2508175201048815 1101 10 -9.295727673663922 1101 11 -4.635431233176181 1101 12 -2.4552795273430057 1101 13 -1.7778053056860763 1101 14 -1.5389052774344698 1101 15 -2.8828434268202194 1101 16 -1.3923850708333525 1101 17 -1.1392999055119297 1101 18 -1.3109684846271712 1101 19 -1.7532807463667943 1101 20 -1.28433328775116 1101 21 -1.257070564114116 1101 22 -1.0195604757042034 1101 23 -1.0167544410421279 1101 24 -1.0663805510048867 1101 25 -1.0663463589835906 1102 1 -1.2058621316666418 1102 2 -0.9492719553704383 1102 3 -1.1787716514793658 1102 4 -1.1167333028979165 1102 5 -1.1955533179029167 1102 6 -1.5100652791001679 1102 7 -0.8226870621474518 1102 8 -0.4187000946753052 1102 9 -0.4286406894973993 1102 10 -0.5429227200918767 1102 11 -0.5816698194211405 1102 12 -0.4173698834681865 1102 13 -0.7675375213425397 1102 14 -0.8744807512578845 1102 15 -0.4244108451506605 1102 16 -0.291131501899315 1102 17 -0.6672410417832353 1102 18 -0.7783746554778972 1102 19 -0.4458514251641468 1102 20 -0.528311227049354 1102 21 -0.7064616891213049 1102 22 -0.6447585403498657 1102 23 -0.9167818708157789 1102 24 -4.040458943601301 1102 25 -2.650694798172914 1103 1 -0.6777949456880634 1103 2 -0.6480304753314918 1103 3 -0.3391538325893926 1103 4 -0.3441554061111596 1103 5 -0.8939101825572493 1103 6 -0.09205164716248504 1103 7 -0.2847488927219395 1103 8 -0.08908714433450626 1103 9 -0.2554055385723757 1103 10 -0.1752012011045352 1103 11 -0.36002911835005946 1103 12 -0.24222382699301548 1103 13 -0.2372308027446799 1103 14 -0.27905718747989283 1103 15 -0.11981006618279366 1103 16 -0.010800440555591706 1103 17 -0.44837541374038636 1103 18 -0.6239199329852028 1103 19 -2.3255739178613197 1103 20 -0.9888272932616218 1103 21 -0.8116101798710602 1103 22 -0.5843763592398368 1103 23 -0.5110978396570651 1103 24 1.0454222637988941 1103 25 0.3523845591785957 1104 1 -1.2187868485220719 1104 2 -0.7138475529906749 1104 3 -2.0936302771974162 1104 4 -1.3688747656989406 1104 5 -1.3950557475186045 1104 6 -2.514981349039555 1104 7 -1.2877439240064454 1104 8 -0.6553320836057601 1104 9 -0.6658904483993915 1104 10 -0.7218151135079225 1104 11 -0.7238771124612136 1104 12 -0.6762043201840342 1104 13 -0.7501402617067718 1104 14 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1108 23 1.01846010240088 1108 24 -0.20985103433154606 1108 25 0.2907444136098789 1109 1 0.5487108430753445 1109 2 0.5529869975003869 1109 3 -0.6348877270421234 1109 4 0.6664144141885799 1109 5 0.9145891781928022 1109 6 0.9315224703174501 1109 7 1.119391138530279 1109 8 1.3985922007894374 1109 9 0.7272095264180329 1109 10 0.8454643291177781 1109 11 0.5146690136195389 1109 12 0.15926953191031856 1109 13 0.2915620802725769 1109 14 0.4798390409454214 1109 15 -0.8410633512172403 1109 16 0.13002632270884362 1109 17 -0.10947883017621507 1109 18 0.3254283872079924 1109 19 -0.9017103622110625 1109 20 0.003796472630838821 1109 21 0.02520617782479917 1109 22 0.4685775082223387 1109 23 0.4252905757133476 1109 24 -0.3860288335801805 1109 25 0.16238438160916502 1110 1 0.20671554382242618 1110 2 0.20347302117200528 1110 3 0.7516328806973185 1110 4 0.534952985497231 1110 5 0.6093940132469449 1110 6 0.7901861150752758 1110 7 0.7038350233619752 1110 8 0.9084478207004594 1110 9 0.3805210348541941 1110 10 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0.6712002938992789 2215 3 1.0700968305405816 2215 4 1.2688087848702736 2215 5 1.6295977750024306 2215 6 1.953571695012581 2215 7 1.6522206032992806 2215 8 0.2010653755593914 2215 9 0.6842827903400881 2215 10 0.49800834796708315 2215 11 0.43194700897089433 2215 12 0.317922061601152 2215 13 0.5043474465921918 2215 14 0.8127863138463667 2215 15 0.6415957693609755 2215 16 0.9697698512215922 2215 17 0.5628314828113332 2215 18 -0.17040534412578268 2215 19 1.0555268778946498 2215 20 -2.7975806089114883 2215 21 -1.238301161303422 2215 22 -0.15045726109659796 2215 23 0.0586302770538083 2215 24 -0.28116924813744504 2215 25 0.16209941329000088 2216 1 0.7085770156839004 2216 2 -0.4107261130271276 2216 3 0.3224553915198314 2216 4 0.1864681370123833 2216 5 0.6809524548053894 2216 6 -0.5960476511584893 2216 7 -8.70118662165967 2216 8 -9.76934438709175 2216 9 -4.760171605838444 2216 10 -2.505820581723917 2216 11 -1.5947360780570214 2216 12 -1.308649068418255 2216 13 -0.9530566435705197 2216 14 -1.19610680452951 2216 15 -2.1966748680475945 2216 16 -0.9941837239388782 2216 17 -1.1560593964243715 2216 18 -1.0256598597251276 2216 19 -0.5121623294497333 2216 20 -1.3260400804744101 2216 21 -3.6492766800375627 2216 22 -2.4848467222102 2216 23 -2.432622148681375 2216 24 -2.784680966304336 2216 25 -2.1129043186102834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60305.0 3 12.0 4 435.0 5 2316.0 6 2767.0 7 2297.0 8 1861.0 9 1647.0 10 1641.0 11 1580.0 12 1564.0 13 1492.0 14 1569.0 15 1581.0 16 1684.0 17 1750.0 18 2075.0 19 2603.0 20 3397.0 21 4404.0 22 5416.0 23 6258.0 24 7357.0 25 8387.0 26 9396.0 27 10231.0 28 11477.0 29 13240.0 30 15886.0 31 19029.0 32 24502.0 33 31715.0 34 42037.0 35 57899.0 36 82515.0 37 125048.0 38 129185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.12177150971982 19.232313175247437 13.170643273675836 25.475272041356906 2 13.240999619069523 20.117872826719758 43.83014813164382 22.810979422566895 3 19.506869243585374 24.753858693542753 29.099947242599228 26.63932482027264 4 11.154929739343812 16.926633249575104 39.64738216021527 32.271054850865816 5 11.848971421514708 38.33856501598224 36.49407379113668 13.318389771366373 6 30.171895125449982 37.99311615377657 19.79313999020696 12.041848730566489 7 26.938067367845136 31.16831882743379 23.929622990590964 17.963990814130113 8 24.270578224681344 38.641321014689936 21.239869441960845 15.848231318667871 9 25.745092070347948 16.04726431024778 21.120323251352623 37.08732036805165 10 13.64210828665805 30.30934941835416 35.88237828522189 20.166164009765904 11 33.25517415664191 23.437271158258692 25.26800508872239 18.039549596377014 12 22.655256091782373 25.531065577301383 32.901942765245614 18.911735565670636 13 28.357958216670255 23.117524588987006 26.0205327015579 22.503984492784838 14 19.19194529879185 24.018792771215818 28.678647632667946 28.110614297324382 15 22.075302104392573 33.135137153096856 23.481724407278072 21.307836335232494 16 20.26314693639012 29.666421124125343 30.011042234753027 20.059389704731505 17 20.22170370104462 30.22665757260294 29.163370068564454 20.38826865778799 18 19.879015525814374 28.932318476623692 31.610747602494353 19.577918395067577 19 22.79601082734407 27.862030342411163 28.17048040954357 21.17147842070119 20 22.172700030010066 28.711304605037384 29.60729890743259 19.50869645751996 21 21.406387527622186 28.13579131913085 28.002400743196574 22.455420410050383 22 21.31969053868554 30.170324337053223 28.48172696414315 20.02825816011809 23 20.666056887512976 29.51824096423602 29.028168063558706 20.787534084692293 24 22.316590230972682 28.15020834350402 29.68643656507468 19.84676486044862 25 21.162784353197434 28.78932987196478 29.429864929090282 20.6180208457475 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 239.0 2 322.0 3 405.0 4 405.0 5 405.0 6 588.5 7 772.0 8 772.0 9 772.0 10 1243.5 11 1715.0 12 1715.0 13 1715.0 14 2908.0 15 4101.0 16 4101.0 17 4101.0 18 6919.5 19 9738.0 20 9738.0 21 9738.0 22 15266.0 23 20794.0 24 20794.0 25 20794.0 26 29796.5 27 38799.0 28 38799.0 29 38799.0 30 50436.0 31 62073.0 32 62073.0 33 62073.0 34 70492.5 35 78912.0 36 78912.0 37 78912.0 38 84353.5 39 89795.0 40 89795.0 41 89795.0 42 91859.0 43 93923.0 44 93923.0 45 93923.0 46 93182.0 47 92441.0 48 92441.0 49 92441.0 50 85559.5 51 78678.0 52 78678.0 53 78678.0 54 66358.5 55 54039.0 56 54039.0 57 54039.0 58 44183.5 59 34328.0 60 34328.0 61 34328.0 62 26807.0 63 19286.0 64 19286.0 65 19286.0 66 14445.5 67 9605.0 68 9605.0 69 9605.0 70 6868.5 71 4132.0 72 4132.0 73 4132.0 74 2926.0 75 1720.0 76 1720.0 77 1720.0 78 1194.0 79 668.0 80 668.0 81 668.0 82 454.0 83 240.0 84 240.0 85 240.0 86 169.5 87 99.0 88 99.0 89 99.0 90 68.0 91 37.0 92 37.0 93 37.0 94 25.0 95 13.0 96 13.0 97 13.0 98 9.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.220369301623124 2 0.12820181521136792 3 0.1322215809738744 4 0.07278647291395691 5 0.022970090071465693 6 0.020816644127265783 7 0.28899244571162774 8 0.28726968895626787 9 0.01923745043485252 10 0.037039270240238426 11 0.016653315301812625 12 0.018376072057172555 13 0.015504810798239342 14 0.01722756755359927 15 0.015791936924132662 16 0.020242391875479143 17 0.019093887371905857 18 0.01507412160939936 19 0.02483640988977228 20 0.017945382868332572 21 0.015361247735292682 22 0.017514693679492592 23 0.018806761246012535 24 0.016079063050025984 25 0.015648373861186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 696558.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.08559511512406 #Duplication Level Percentage of deduplicated Percentage of total 1 94.85532156211633 82.6053212807336 2 3.953428441683973 6.885733371782128 3 0.5481170265739372 1.4319929235577078 4 0.20243239298667184 0.7051578165529194 5 0.10627479904159226 0.4627502060138639 6 0.06168223742220211 0.3222980612366908 7 0.04237219309427283 0.258300535636339 8 0.03296073273978644 0.22963240208598848 9 0.024077669268440165 0.18871363415045211 >10 0.14564517865193577 2.4093919967264497 >50 0.014997803874822722 0.9339198797706209 >100 0.010877110756427581 1.9668139903417006 >500 8.240235407659096E-4 0.451661093267148 >1k 9.888282489190915E-4 1.1483128081444078 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1769 0.25396305835264255 No Hit GTCCTACAGTGTGCATTTCTCATTT 1501 0.21548815748293754 No Hit CTGTAGGACGTGGAATATGGCAAGA 1464 0.21017632415391108 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1220 0.1751469367949259 No Hit GTATCAACGCAGAGTACTTTTTTTT 1045 0.1500234007792603 No Hit CTTTAGGACGTGAAATATGGCGAGG 1002 0.1438501890725539 No Hit CTGTAGGACCTGGAATATGGCGAGA 941 0.1350928422328076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.435630629466606E-4 0.0 3 0.0 0.0 0.0 1.435630629466606E-4 0.0 4 0.0 0.0 0.0 1.435630629466606E-4 0.0 5 0.0 0.0 0.0 1.435630629466606E-4 0.0 6 0.0 0.0 0.0 1.435630629466606E-4 0.0 7 0.0 0.0 0.0 1.435630629466606E-4 0.0 8 0.0 0.0 0.0 1.435630629466606E-4 0.0 9 0.0 0.0 0.0 1.435630629466606E-4 0.0 10 0.0 0.0 0.0 1.435630629466606E-4 0.0 11 0.0 0.0 0.0 1.435630629466606E-4 0.0 12 0.0 0.0 0.0 1.435630629466606E-4 0.0 13 0.0 0.0 0.0 1.435630629466606E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTT 50 8.3701656E-5 15.269606 1 TAGGACC 390 0.0 13.428704 4 CGCCTTG 65 5.272393E-5 13.198821 2 CTGGACT 80 1.9433173E-6 13.092986 4 GTCCTAT 110 2.5538611E-9 13.013869 1 AGGACCT 520 0.0 12.444893 5 CTAGACA 55 0.0030124418 12.119128 4 CCAACGA 55 0.0031064325 12.069524 19 GTCTAGT 80 2.7302765E-5 11.9293785 1 GTCCTAC 970 0.0 11.806396 1 GACGTGG 370 0.0 11.574344 7 TGTAGGA 960 0.0 11.5184145 2 GTAGGAC 950 0.0 11.438978 3 GGACTTG 125 1.7771526E-8 11.420018 6 GGACCTG 500 0.0 11.420018 6 GGACGTG 690 0.0 11.309681 6 ATAGGAC 110 4.738613E-7 11.26566 3 GTCCTAG 145 1.1223165E-9 11.188934 1 CTGTAGG 950 0.0 11.15083 1 TCCAACG 60 0.0059479955 11.063729 18 >>END_MODULE