FastQCFastQC Report
Thu 26 May 2016
SRR1926950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696558
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18900.2713341889691885No Hit
GTCCTACAGTGGACATTTCTAAATT18850.27061637365445523No Hit
GTATCAACGCAGAGTACTTTTTTTT17220.2472155943941495No Hit
GTCCTACAGTGTGCATTTCTCATTT15390.2209435538749106No Hit
CTTTAGGACGTGAAATATGGCGAGG12570.18045877012395237No Hit
GTCCTAAAGTGTGTATTTCTCATTT12440.17859245030564574No Hit
CTGTAGGACCTGGAATATGGCGAGA12260.17600831517260587No Hit
GGTATCAACGCAGAGTACTTTTTTT11020.15820649536721995No Hit
TATCAACGCAGAGTACTTTTTTTTT8620.12375136026002143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG1700.014.5776111
CCTAGAC602.504029E-514.2859413
CTAGGGG400.005187780614.2859413
AGGACCG400.005190261614.2849115
GGACCGA400.005190261614.2849116
TAAGGAG954.7184585E-914.0342995
TAGGACC3850.013.10996154
AATCCCG802.0207972E-613.04749419
AGGACCT6100.012.9578975
AAGGACC2300.012.8357184
CTAGTCC604.0010223E-412.6986153
GGACCTG5550.012.6976996
TATAGCC701.05748186E-412.2530382
CTTAGAC701.0639086E-412.2450913
TAGGACT1501.2732926E-1112.0628154
TTAACCT657.8507775E-411.7209534
TGTAGGA10600.011.6879092
CTAGACT907.2437906E-611.6395574
CTGTAGG10050.011.5705971
GTCCTAC9900.011.5533521
Produced by FastQC (version 0.11.5)