##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926950_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 696558 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.729323329859106 31.0 30.0 33.0 25.0 34.0 2 30.05224403423692 31.0 30.0 34.0 26.0 34.0 3 30.125471245754124 31.0 30.0 34.0 26.0 34.0 4 33.669051249142214 35.0 35.0 37.0 28.0 37.0 5 33.599107324874595 37.0 35.0 37.0 28.0 37.0 6 33.538858214247774 37.0 35.0 37.0 28.0 37.0 7 33.5251594267814 37.0 35.0 37.0 28.0 37.0 8 33.546485719782126 37.0 35.0 37.0 28.0 37.0 9 35.057636262881196 39.0 35.0 39.0 28.0 39.0 10 34.96087475845515 39.0 35.0 39.0 28.0 39.0 11 34.98312846884251 39.0 35.0 39.0 28.0 39.0 12 34.882882688878745 39.0 35.0 39.0 27.0 39.0 13 34.89877655557757 39.0 35.0 39.0 27.0 39.0 14 35.977907080243135 40.0 36.0 41.0 27.0 41.0 15 35.96132267521154 40.0 36.0 41.0 27.0 41.0 16 35.94686156788092 40.0 36.0 41.0 27.0 41.0 17 35.91790776934584 40.0 36.0 41.0 27.0 41.0 18 35.89997100026128 40.0 36.0 41.0 27.0 41.0 19 35.96989769696135 40.0 36.0 41.0 27.0 41.0 20 35.930783940461524 40.0 36.0 41.0 27.0 41.0 21 35.88081250951105 40.0 36.0 41.0 27.0 41.0 22 35.782170328960405 40.0 36.0 41.0 27.0 41.0 23 35.70713278721944 40.0 36.0 41.0 27.0 41.0 24 35.64521834506244 40.0 36.0 41.0 27.0 41.0 25 35.52701139029342 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.015950860553250124 1101 2 -0.12056338976437786 1101 3 0.1441934200664825 1101 4 0.011464785433489055 1101 5 -0.19152907965307264 1101 6 0.008323820289369621 1101 7 0.10413564462888303 1101 8 -0.025951558520795004 1101 9 -0.03548042383163619 1101 10 0.020742465303918323 1101 11 -0.14151181709319616 1101 12 -0.008003539346290722 1101 13 -0.09374724390349343 1101 14 -0.05390722429862649 1101 15 -0.4824401705875232 1101 16 -0.32694367453621 1101 17 -0.11317249476339697 1101 18 -0.2552100035489886 1101 19 -0.30599505528507365 1101 20 -0.3357356500222508 1101 21 -0.27193723068211995 1101 22 -0.3393708137896354 1101 23 -0.277038582353903 1101 24 -0.30949296306099683 1101 25 -0.4718638082416362 1102 1 -0.5864512712397456 1102 2 -0.4793297474769034 1102 3 -0.32648924196006135 1102 4 -0.4072229025570806 1102 5 -0.5719404508495458 1102 6 -0.7841826671820087 1102 7 -0.5251674600255996 1102 8 -0.41438543155770446 1102 9 -0.5475952405281674 1102 10 -0.6542363968034977 1102 11 -0.5505651505647791 1102 12 -0.5338228742261251 1102 13 -0.38025452881777255 1102 14 -0.5003018114899618 1102 15 -0.4788422514799109 1102 16 -0.4588733449736111 1102 17 -0.4806447421479092 1102 18 -0.6216906316248227 1102 19 -0.5797244016552412 1102 20 -0.5962232967267695 1102 21 -0.5264186053002433 1102 22 -0.5597770572208631 1102 23 -0.6670626281795293 1102 24 -0.5394147929191035 1102 25 -0.6382720443375263 1103 1 -0.5206406578025486 1103 2 -0.39793977668819736 1103 3 -0.33934221177505464 1103 4 -0.5343772355658416 1103 5 -0.6200904021640596 1103 6 -0.7451490741927174 1103 7 -0.48463678816581535 1103 8 -0.571712598062085 1103 9 -0.5740071197881491 1103 10 -0.4222987142329089 1103 11 -0.30566495582281306 1103 12 -0.5289786677996418 1103 13 -0.34427595043413106 1103 14 -0.6005208962027169 1103 15 -0.46527117066199253 1103 16 -0.44530226415569274 1103 17 -0.6019933302686482 1103 18 -0.7092758312353382 1103 19 -0.7084244990262292 1103 20 -0.49636935320193487 1103 21 -0.5317983521356879 1103 22 -0.5163739413493929 1103 23 -0.6211278667384406 1103 24 -0.6135141113222176 1103 25 -0.6710130375215542 1104 1 -0.2947343272909535 1104 2 -0.29577286465286434 1104 3 -0.11151740500287843 1104 4 -0.25266486898505036 1104 5 -0.41893103521521624 1104 6 -0.23109405026927732 1104 7 -0.3163537554090965 1104 8 -0.2940442138147503 1104 9 -0.5121466118750106 1104 10 -0.32815075692705165 1104 11 -0.24084622192513194 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17 0.5713265808671508 2208 18 0.3609407752628613 2208 19 0.597886247988491 2208 20 0.5302482530185557 2208 21 0.4666951218992992 2208 22 0.4060850978063968 2208 23 0.5572878511177137 2208 24 0.42121235354864695 2208 25 0.7318958766689505 2209 1 0.34140146673957616 2209 2 0.3203294540810333 2209 3 0.49875302632018403 2209 4 0.24277477908617584 2209 5 0.3114114293377739 2209 6 0.11689305034940389 2209 7 0.22003354150451315 2209 8 0.29797604662655885 2209 9 0.3293811586001283 2209 10 0.209386267452075 2209 11 0.3006079379500832 2209 12 0.2648858010648283 2209 13 0.3570061008479186 2209 14 0.4153215946397708 2209 15 0.4877965949057739 2209 16 0.40611714976372326 2209 17 0.4259370726617533 2209 18 0.1782699563104515 2209 19 0.029945464343732908 2209 20 0.14392368709110315 2209 21 0.1686595233221837 2209 22 0.009541809566357529 2209 23 0.21925674864617406 2209 24 0.1910646432565386 2209 25 0.29647780496928533 2210 1 0.1433012292692588 2210 2 0.15791946686789515 2210 3 0.26536873781778425 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14 -0.16409173921405795 2211 15 -0.060391747243492944 2211 16 0.099896875070975 2211 17 -0.18190520347536676 2211 18 -0.2162918170257484 2211 19 -0.16757533930495327 2211 20 -0.16515607269913346 2211 21 -0.18005289510603717 2211 22 -0.16349444961555548 2211 23 -0.2938256396582375 2211 24 -0.11410162327740636 2211 25 -0.033613802653405855 2212 1 -0.1579719668443822 2212 2 -0.194177968321128 2212 3 -0.06255408761850845 2212 4 -0.1126329710584173 2212 5 -0.21199323617215526 2212 6 -0.32607012682429826 2212 7 -0.31699171394179615 2212 8 -0.5293711675627577 2212 9 -0.46095043962620963 2212 10 -0.5629194969589548 2212 11 -0.49626886945303994 2212 12 -0.3973659822395845 2212 13 -0.5786889058997176 2212 14 -0.4363845939085209 2212 15 -0.47284539468358133 2212 16 -0.6449817513351803 2212 17 -0.5185587823604152 2212 18 -0.5268196908844587 2212 19 -0.5956377243973492 2212 20 -0.6585825785730606 2212 21 -0.8728577313529655 2212 22 -0.592993374547774 2212 23 -0.5284490543227847 2212 24 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0.17356709396770498 2214 11 0.1885869926692223 2214 12 -0.04211828148991259 2214 13 0.04909917664143393 2214 14 0.003011804576679822 2214 15 0.029616155668065858 2214 16 0.1112590709849357 2214 17 0.10506359958564104 2214 18 0.05349949916114127 2214 19 0.022675128218772045 2214 20 0.2192016396818275 2214 21 0.33157846928077817 2214 22 0.20430548935205906 2214 23 0.22906698246521984 2214 24 0.28980679684450195 2214 25 0.31696561378308985 2215 1 0.3911962122239778 2215 2 0.2920138042136564 2215 3 0.3872493001612831 2215 4 0.2917832418877424 2215 5 0.378251772902253 2215 6 0.1570060982210748 2215 7 0.07962050626389328 2215 8 0.16701419238884085 2215 9 0.03501144785110455 2215 10 0.16496919027637347 2215 11 0.01817456672360862 2215 12 0.1433024993091152 2215 13 0.15393359863748657 2215 14 0.21115019133043944 2215 15 0.2192447444573702 2215 16 0.17455847854987638 2215 17 0.15019499864381913 2215 18 0.3071034791094931 2215 19 -0.014724233774238371 2215 20 0.16715849825694562 2215 21 0.24857300064945775 2215 22 0.20981969260285638 2215 23 0.28523818100691756 2215 24 0.10206944305443955 2215 25 0.23495513223971898 2216 1 0.19594692128503155 2216 2 0.07009635384793 2216 3 -1.8520961245289698 2216 4 -1.3274882911768984 2216 5 -0.5656614977351495 2216 6 -0.37085253739618196 2216 7 -0.23556086408989074 2216 8 -0.06456141591090159 2216 9 -0.17416529494632726 2216 10 -0.08909858103277202 2216 11 -0.2970777097355679 2216 12 -0.2186806325016093 2216 13 -0.39740615356434006 2216 14 -0.47806834875017046 2216 15 -0.3160329089237308 2216 16 -0.3852414915949183 2216 17 -0.18819879147410745 2216 18 -0.236765008724511 2216 19 -0.1275470431487733 2216 20 -0.17447458130716598 2216 21 -0.037451032788368366 2216 22 -0.21879651877197404 2216 23 -0.32441624502681776 2216 24 -0.6168774146855256 2216 25 -0.5445977861063014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19113.0 3 17.0 4 330.0 5 2147.0 6 2862.0 7 2037.0 8 1552.0 9 1399.0 10 1274.0 11 1133.0 12 1033.0 13 954.0 14 900.0 15 979.0 16 942.0 17 985.0 18 1139.0 19 1346.0 20 1749.0 21 2294.0 22 2816.0 23 3514.0 24 4185.0 25 4798.0 26 5725.0 27 6235.0 28 7352.0 29 8684.0 30 11201.0 31 14305.0 32 18596.0 33 24445.0 34 33120.0 35 45461.0 36 67382.0 37 116613.0 38 277941.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.79670566590968 20.183976772932414 10.685457211027108 28.333860350130795 2 13.177187155522915 21.22144118792586 41.873754133725015 23.72761752282621 3 18.32400742626503 25.09465903363788 29.425067521290206 27.156266018806885 4 11.411684310567102 17.93863540437408 39.133424639441365 31.516255645617452 5 11.953747067556044 39.40759607074045 36.126273836426336 12.512383025277165 6 30.54812083147265 39.11998156124404 18.205390455062734 12.126507152220574 7 27.638955101290613 32.13255946192771 22.007231351556264 18.221254085225414 8 23.70864920744314 40.034602572938205 20.124195956811395 16.13255226280726 9 25.751997909481705 16.259953652520252 20.593184565772358 37.394863872225685 10 13.813822005464255 30.962618821668077 34.848326051969266 20.3752331208984 11 33.40645835606526 23.556977218301164 24.932234926578264 18.104329499055307 12 22.792998696443647 26.001068112255155 32.419360396005494 18.78657279529571 13 28.5306975041454 23.201326528414842 26.071020953119277 22.196955014320476 14 19.395226241030898 24.114431246213524 28.23828022935635 28.252062283399226 15 21.39538689920722 33.53700731331241 23.49749762396309 21.570108163517283 16 20.14026171659094 30.084917917465237 29.648771453797618 20.126048912146207 17 19.889829547330656 30.628649198845174 28.95742918382846 20.52409206999571 18 19.794129681073283 29.115719505279554 31.53907444494692 19.551076368700247 19 22.140789828814913 28.13097582965332 28.556210843062154 21.172023498469613 20 22.08471992121214 28.941474745676572 29.57645781949425 19.397347513617035 21 21.25974190065415 28.044756746822575 28.19436707079641 22.501134281726863 22 21.420644685215997 30.209444453217753 28.326612179620618 20.043298681945625 23 20.35928143712575 29.68673059060493 29.091947966928767 20.862040005340553 24 21.639147980710842 28.135098778707267 30.138237071768714 20.087516168813178 25 20.90542459365439 29.14906110869431 29.41868995984282 20.526824337808485 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 280.0 1 280.0 2 275.5 3 271.0 4 271.0 5 271.0 6 482.5 7 694.0 8 694.0 9 694.0 10 1134.0 11 1574.0 12 1574.0 13 1574.0 14 2630.5 15 3687.0 16 3687.0 17 3687.0 18 6513.5 19 9340.0 20 9340.0 21 9340.0 22 14923.0 23 20506.0 24 20506.0 25 20506.0 26 29262.5 27 38019.0 28 38019.0 29 38019.0 30 50086.5 31 62154.0 32 62154.0 33 62154.0 34 70599.5 35 79045.0 36 79045.0 37 79045.0 38 84375.0 39 89705.0 40 89705.0 41 89705.0 42 91980.0 43 94255.0 44 94255.0 45 94255.0 46 94431.0 47 94607.0 48 94607.0 49 94607.0 50 87281.0 51 79955.0 52 79955.0 53 79955.0 54 66981.5 55 54008.0 56 54008.0 57 54008.0 58 44340.5 59 34673.0 60 34673.0 61 34673.0 62 26772.0 63 18871.0 64 18871.0 65 18871.0 66 13992.0 67 9113.0 68 9113.0 69 9113.0 70 6457.0 71 3801.0 72 3801.0 73 3801.0 74 2578.0 75 1355.0 76 1355.0 77 1355.0 78 914.0 79 473.0 80 473.0 81 473.0 82 300.0 83 127.0 84 127.0 85 127.0 86 81.5 87 36.0 88 36.0 89 36.0 90 22.0 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11743458549036835 2 0.10867723865062207 3 0.015935499987079323 4 0.0 5 0.005742522517866423 6 0.33995733305769227 7 0.020960207190212445 8 0.011197918909839524 9 0.011485045035732847 10 0.004450454951346478 11 0.004881144140186459 12 2.871261258933212E-4 13 4.306891888399818E-4 14 0.0 15 0.0028712612589332116 16 4.306891888399818E-4 17 1.435630629466606E-4 18 4.306891888399818E-4 19 2.871261258933212E-4 20 5.742522517866423E-4 21 0.011772171161626169 22 1.435630629466606E-4 23 1.435630629466606E-4 24 1.435630629466606E-4 25 0.006460337832599725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 696558.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.62738772314381 #Duplication Level Percentage of deduplicated Percentage of total 1 92.90232024181913 77.6917835524228 2 5.50655585296252 9.20997762669687 3 0.7841682954639795 1.9673383825488902 4 0.2514074914957625 0.8409820707127644 5 0.13444155703971242 0.5621498108331592 6 0.08317360624125943 0.41733548504819373 7 0.05590042622959125 0.32723646327337186 8 0.042257144194336765 0.28270836652900766 9 0.034886925465521135 0.26257521981362 >10 0.16982733258864993 2.7075117851808965 >50 0.01942138710965241 1.1366169529325565 >100 0.013061760370449002 2.246300437495168 >500 0.0012030568757292728 0.6421697071094848 >1k 0.0013749221436905976 1.7053141394033207 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1890 0.2713341889691885 No Hit GTCCTACAGTGGACATTTCTAAATT 1885 0.27061637365445523 No Hit GTATCAACGCAGAGTACTTTTTTTT 1722 0.2472155943941495 No Hit GTCCTACAGTGTGCATTTCTCATTT 1539 0.2209435538749106 No Hit CTTTAGGACGTGAAATATGGCGAGG 1257 0.18045877012395237 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1244 0.17859245030564574 No Hit CTGTAGGACCTGGAATATGGCGAGA 1226 0.17600831517260587 No Hit GGTATCAACGCAGAGTACTTTTTTT 1102 0.15820649536721995 No Hit TATCAACGCAGAGTACTTTTTTTTT 862 0.12375136026002143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.435630629466606E-4 0.0 8 0.0 0.0 0.0 1.435630629466606E-4 0.0 9 0.0 0.0 0.0 1.435630629466606E-4 0.0 10 0.0 0.0 0.0 2.871261258933212E-4 0.0 11 0.0 0.0 0.0 2.871261258933212E-4 0.0 12 0.0 0.0 0.0 4.3068918883998173E-4 0.0 13 0.0 0.0 0.0 4.3068918883998173E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 170 0.0 14.577611 1 CCTAGAC 60 2.504029E-5 14.285941 3 CTAGGGG 40 0.0051877806 14.285941 3 AGGACCG 40 0.0051902616 14.284911 5 GGACCGA 40 0.0051902616 14.284911 6 TAAGGAG 95 4.7184585E-9 14.034299 5 TAGGACC 385 0.0 13.1099615 4 AATCCCG 80 2.0207972E-6 13.047494 19 AGGACCT 610 0.0 12.957897 5 AAGGACC 230 0.0 12.835718 4 CTAGTCC 60 4.0010223E-4 12.698615 3 GGACCTG 555 0.0 12.697699 6 TATAGCC 70 1.05748186E-4 12.253038 2 CTTAGAC 70 1.0639086E-4 12.245091 3 TAGGACT 150 1.2732926E-11 12.062815 4 TTAACCT 65 7.8507775E-4 11.720953 4 TGTAGGA 1060 0.0 11.687909 2 CTAGACT 90 7.2437906E-6 11.639557 4 CTGTAGG 1005 0.0 11.570597 1 GTCCTAC 990 0.0 11.553352 1 >>END_MODULE