FastQCFastQC Report
Thu 26 May 2016
SRR1926949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT21520.349202691716659No Hit
CTGTAGGACGTGGAATATGGCAAGA18510.30035975017078803No Hit
GTCCTAAAGTGTGTATTTCTCATTT17420.2826724391126487No Hit
GTCCTACAGTGTGCATTTCTCATTT16520.2680682373215245No Hit
CTTTAGGACGTGAAATATGGCGAGG13540.2197121025020243No Hit
CTGTAGGACCTGGAATATGGCGAGA11050.1793071442132473No Hit
GTATCAACGCAGAGTACTTTTTTTT9550.15496680789470696No Hit
GTCCTTCAGTGTGCATTTCTCATTT6680.10839563107189973No Hit
CTGAAGGACCTGGAATATGGCGAGA6680.10839563107189973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC508.5028994E-515.2409423
TAGGACC3400.014.28144
CGGTTTC400.005334175714.225780513
GTTCCAA655.2699543E-513.19895651
GGCGAGG4450.013.0003419
CTAAGAC603.9940837E-412.7007853
AGGGGAG751.4457559E-512.6914775
TCCAACG751.5011801E-512.64513918
CCTATAC550.003003679212.1234763
CCAACGA550.003104125812.0703619
ACTGATC657.9294655E-411.706638
GTCCTAA5150.011.6612151
GTGTAGG1001.845523E-611.4390961
ATACCTG752.0326416E-411.4223295
GACGTGA5350.011.3867457
GTCCTAC11050.011.3010681
TGGCGAG9850.011.26508518
TGTAGGA11600.011.2500492
GTAGGAC11200.011.2265873
CCTACAC1201.2231067E-711.1131873
Produced by FastQC (version 0.11.5)