##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926949_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616261 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.38676307603434 30.0 26.0 31.0 10.0 31.0 2 27.02402228925731 31.0 27.0 31.0 10.0 34.0 3 26.636047064474305 31.0 27.0 31.0 10.0 34.0 4 30.31533717045213 35.0 30.0 37.0 10.0 37.0 5 29.887810521840585 35.0 30.0 37.0 10.0 37.0 6 29.68611026821428 35.0 30.0 37.0 8.0 37.0 7 29.732118371923583 35.0 30.0 37.0 8.0 37.0 8 29.431064110823176 35.0 29.0 37.0 2.0 37.0 9 31.612208139083926 37.0 32.0 39.0 2.0 39.0 10 31.78416774710715 37.0 32.0 39.0 2.0 39.0 11 32.00145555211185 37.0 32.0 39.0 2.0 39.0 12 31.864135488048085 37.0 32.0 39.0 2.0 39.0 13 31.98102751918424 37.0 32.0 39.0 2.0 39.0 14 32.88538297896508 38.0 32.0 40.0 2.0 41.0 15 32.47224958256323 38.0 32.0 40.0 2.0 41.0 16 32.54957234029089 38.0 32.0 40.0 2.0 41.0 17 32.760124362891695 38.0 32.0 40.0 2.0 41.0 18 32.80394832708868 38.0 32.0 40.0 2.0 41.0 19 32.15901379447994 38.0 31.0 40.0 2.0 41.0 20 32.46120231525279 38.0 32.0 40.0 2.0 41.0 21 32.43149087805329 38.0 32.0 40.0 2.0 41.0 22 32.45589936731353 39.0 32.0 40.0 2.0 41.0 23 32.42417741833412 39.0 32.0 40.0 2.0 41.0 24 31.126144604315378 38.0 30.0 40.0 2.0 41.0 25 31.659202837758677 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.6817073482930027 1101 2 -1.5260298376897374 1101 3 -2.578955716398468 1101 4 -2.252264021411154 1101 5 -2.247310197769149 1101 6 -3.964144584335031 1101 7 -2.1884594677390226 1101 8 -1.8737407269995998 1101 9 -1.9585000413609528 1101 10 -9.780198349883435 1101 11 -5.554308833621281 1101 12 -3.508377452903435 1101 13 -2.761701244369945 1101 14 -2.460284740736288 1101 15 -3.386286505831375 1101 16 -2.0191970037796025 1101 17 -1.877388135597137 1101 18 -1.8799672361353075 1101 19 -2.37028095492024 1101 20 -1.9259380650667843 1101 21 -1.9078442544417413 1101 22 -1.611349518441667 1101 23 -1.9531907342019466 1101 24 -2.151148139018485 1101 25 -2.0217283491142233 1102 1 -1.1668387130918845 1102 2 -1.0957403701240551 1102 3 -1.239877595031345 1102 4 -1.3451493969052244 1102 5 -1.464963069194397 1102 6 -2.018050652691226 1102 7 -0.946527358927117 1102 8 -0.558290831626433 1102 9 -0.7431024594032678 1102 10 -0.712748919518404 1102 11 -0.8200900472925206 1102 12 -0.9146783131684906 1102 13 -1.1426417233283246 1102 14 -1.4006183840750488 1102 15 -0.8485569080634114 1102 16 -1.0967719863417962 1102 17 -1.1039633506634026 1102 18 -1.1593673756377498 1102 19 -1.01006821369727 1102 20 -0.9205788462804563 1102 21 -0.9648193765063766 1102 22 -0.9950451706155832 1102 23 -1.4494822943042465 1102 24 -4.889987943715067 1102 25 -3.16629356650553 1103 1 -1.4960722246783398 1103 2 -1.5510582548537037 1103 3 -1.0690120771070042 1103 4 -1.736890335704988 1103 5 -2.2121605340389223 1103 6 -1.194659882241634 1103 7 -1.2940813300104992 1103 8 -1.2037255711788184 1103 9 -1.2817239686130115 1103 10 -1.5623428985241468 1103 11 -1.61000884309367 1103 12 -1.7309785706452168 1103 13 -1.5172307508585305 1103 14 -1.7589467659328406 1103 15 -1.6312069377722658 1103 16 -1.5213709168230807 1103 17 -1.7153913562090537 1103 18 -2.0185672171905296 1103 19 -3.4993730265314937 1103 20 -2.73850318773421 1103 21 -2.49284472806114 1103 22 -2.147768482162423 1103 23 -2.060157675567865 1103 24 -0.28327374288322105 1103 25 -1.4671650262005187 1104 1 -1.3115552213327248 1104 2 -1.1567768561891256 1104 3 -2.2694360431897174 1104 4 -1.4858915920750668 1104 5 -1.7438568218653074 1104 6 -3.0150742490922866 1104 7 -1.497441531782595 1104 8 -1.188235045714972 1104 9 -1.311460413226854 1104 10 -1.2326539795200198 1104 11 -1.2158662568535092 1104 12 -1.2102743446303563 1104 13 -1.2652344399751634 1104 14 -1.4176117197953566 1104 15 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22 -0.3731763833392421 2213 23 -0.36331693352773087 2213 24 -0.660565547610414 2213 25 -0.40625380507411535 2214 1 0.8024362551783497 2214 2 0.48634333272604735 2214 3 0.553537483377017 2214 4 0.6644019256550351 2214 5 0.9392523427083788 2214 6 0.631354314676237 2214 7 1.0466922960017513 2214 8 -2.26968433910616 2214 9 -1.215400650142957 2214 10 -0.4739637313436482 2214 11 0.11924476941830875 2214 12 -1.2823293548074979 2214 13 -0.2010659091080811 2214 14 0.10870299727157118 2214 15 -1.4678082449618088 2214 16 -0.05165890093429937 2214 17 0.21544464364382776 2214 18 0.34765246940417427 2214 19 0.9961874618147917 2214 20 -0.8805924409572903 2214 21 -0.2953080945177291 2214 22 0.18588829512423644 2214 23 -0.4179267127866346 2214 24 -1.6579289343209354 2214 25 -0.7966808554826876 2215 1 1.1708185316241817 2215 2 0.5772517358092273 2215 3 0.8340567059617818 2215 4 1.174071382315553 2215 5 1.640898911961699 2215 6 2.1423771547954047 2215 7 1.4024515053458657 2215 8 0.31977894173745725 2215 9 0.610806376858509 2215 10 0.39272090477494714 2215 11 0.4015193237286141 2215 12 0.013154638193874746 2215 13 0.30348509103378163 2215 14 0.7265372054334804 2215 15 0.48457270741914016 2215 16 0.763390987938827 2215 17 0.3108520300343329 2215 18 -0.2654330746446263 2215 19 0.8963132479783127 2215 20 -3.238546760718961 2215 21 -1.505007815518347 2215 22 -0.41123564680605895 2215 23 -0.5591741710549485 2215 24 -0.33311501272449107 2215 25 0.08799214778018438 2216 1 1.1352882949896532 2216 2 0.29636946879199044 2216 3 1.2025171208025647 2216 4 0.9437704766772264 2216 5 1.103656726744056 2216 6 -0.318154542546111 2216 7 -8.242789511306196 2216 8 -8.875932422562862 2216 9 -4.2081243856305335 2216 10 -1.9036623847371743 2216 11 -0.9603971238320028 2216 12 -0.47045622946607324 2216 13 -0.06977228553647663 2216 14 -0.2568282634534569 2216 15 -1.4473583472112992 2216 16 -0.4661561928966975 2216 17 -0.6746051712510877 2216 18 -0.3494879972271505 2216 19 0.3022028185304606 2216 20 -0.6282809120482042 2216 21 -3.5572485400290113 2216 22 -1.8255155182638418 2216 23 -1.7326652607423547 2216 24 -2.0770706072322334 2216 25 -1.3372547116998028 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52957.0 3 11.0 4 380.0 5 1950.0 6 2466.0 7 1954.0 8 1618.0 9 1477.0 10 1399.0 11 1352.0 12 1315.0 13 1267.0 14 1399.0 15 1430.0 16 1578.0 17 1641.0 18 1940.0 19 2282.0 20 3157.0 21 3904.0 22 4803.0 23 5723.0 24 6745.0 25 7612.0 26 8421.0 27 9217.0 28 10445.0 29 12107.0 30 14335.0 31 17345.0 32 22003.0 33 28848.0 34 38290.0 35 51945.0 36 73432.0 37 109436.0 38 110077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.10278132342539 19.006333384824266 13.002544736136064 25.888340555614274 2 13.19491153677438 19.99593832756576 43.89270686097708 22.91644327468278 3 19.50953218065489 24.709257929403048 29.137895739032388 26.643314150909674 4 11.354388243890558 16.882520094178776 39.1858407079646 32.577250953966065 5 11.864800162304727 38.28800973828363 36.50460539663218 13.342584702779469 6 30.53003338575725 37.769240632268136 19.573597173328398 12.127128808646214 7 26.783712321523 30.875645771468086 24.257250945775535 18.083390961233373 8 24.40874420784089 38.90541208934243 21.030255896287812 15.655587806528871 9 26.10451283283075 15.614278248619664 20.868159583480754 37.413049335068834 10 14.189847280660745 29.845852163523972 35.58500207781414 20.379298478001143 11 33.48101912936789 22.49498111731608 25.886885663604215 18.13711408971182 12 23.58333171038879 24.93995105199265 32.79208132899682 18.68463590862173 13 29.042560960766018 23.05919584533615 25.70211384752708 22.196129346370753 14 19.363063320393138 24.00984796354241 27.984251048415366 28.642837667649086 15 21.87299165828167 33.98211561556688 22.94816449738713 21.19672822876432 16 20.229976466769457 28.659904244096406 30.74640915361519 20.363710135518946 17 20.23744025448142 30.156697584211766 29.276886497715672 20.32897566359114 18 20.047648646411666 28.20991370985934 32.22515608339812 19.51728156033088 19 23.569472780420668 27.001601963257098 27.612685211785386 21.81624004453685 20 22.930096532741285 28.354464071253755 29.15295278529742 19.562486610707538 21 21.408783515126338 27.574544327360485 27.50751813196533 23.509154025547854 22 22.02696364436034 30.22901407582085 27.715718081092177 20.028304198726634 23 20.44407368877189 29.59966502152853 29.08632349766862 20.869937792030953 24 23.2484164298547 27.61571057385044 29.478992033055757 19.6568809632391 25 21.32213083359841 28.687931291912 29.27169686386269 20.718241010626905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 252.0 1 252.0 2 297.0 3 342.0 4 342.0 5 342.0 6 506.5 7 671.0 8 671.0 9 671.0 10 1025.5 11 1380.0 12 1380.0 13 1380.0 14 2292.5 15 3205.0 16 3205.0 17 3205.0 18 5510.0 19 7815.0 20 7815.0 21 7815.0 22 12510.0 23 17205.0 24 17205.0 25 17205.0 26 25208.0 27 33211.0 28 33211.0 29 33211.0 30 44423.5 31 55636.0 32 55636.0 33 55636.0 34 62417.5 35 69199.0 36 69199.0 37 69199.0 38 73352.5 39 77506.0 40 77506.0 41 77506.0 42 79299.0 43 81092.0 44 81092.0 45 81092.0 46 81389.5 47 81687.0 48 81687.0 49 81687.0 50 75548.5 51 69410.0 52 69410.0 53 69410.0 54 59003.0 55 48596.0 56 48596.0 57 48596.0 58 40385.0 59 32174.0 60 32174.0 61 32174.0 62 25723.0 63 19272.0 64 19272.0 65 19272.0 66 14627.0 67 9982.0 68 9982.0 69 9982.0 70 7272.5 71 4563.0 72 4563.0 73 4563.0 74 3235.5 75 1908.0 76 1908.0 77 1908.0 78 1334.0 79 760.0 80 760.0 81 760.0 82 500.0 83 240.0 84 240.0 85 240.0 86 170.5 87 101.0 88 101.0 89 101.0 90 69.0 91 37.0 92 37.0 93 37.0 94 24.0 95 11.0 96 11.0 97 11.0 98 8.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20543243852848064 2 0.12186395050149205 3 0.12446025304213637 4 0.06669252151280058 5 0.02206857159547659 6 0.021094958142734977 7 0.27358538022039364 8 0.26563420368967045 9 0.01736277324055879 10 0.037159580112971616 11 0.014279663973543676 12 0.015740084152656098 13 0.01395512615596314 14 0.015091008517495022 15 0.013143781612011794 16 0.018011848875719865 17 0.01720050433176852 18 0.0136305883383826 19 0.023042185048218207 20 0.015740084152656098 21 0.0136305883383826 22 0.01590235306144637 23 0.016551428696607442 24 0.014604201791124215 25 0.014441932882333945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 616261.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.62749890747665 #Duplication Level Percentage of deduplicated Percentage of total 1 94.77678761537632 80.20722490374406 2 3.85787386223813 6.529644321234801 3 0.6080222953969993 1.5436621841829299 4 0.22101015558575843 0.7481414680150006 5 0.11998260026752322 0.5076913686528015 6 0.08261052622859164 0.4194673330893726 7 0.05030042390054194 0.29797593480821083 8 0.03807199670473127 0.25775502876280804 9 0.032477530200466266 0.2473642936401749 >10 0.17582747427041914 2.9518532606158776 >50 0.01803808127738253 1.1020142672350914 >100 0.016694718728817504 2.952169909850801 >500 0.0011513599121459825 0.6344753747103538 >1k 0.0011513599121459825 1.6005603514577034 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2152 0.349202691716659 No Hit CTGTAGGACGTGGAATATGGCAAGA 1851 0.30035975017078803 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1742 0.2826724391126487 No Hit GTCCTACAGTGTGCATTTCTCATTT 1652 0.2680682373215245 No Hit CTTTAGGACGTGAAATATGGCGAGG 1354 0.2197121025020243 No Hit CTGTAGGACCTGGAATATGGCGAGA 1105 0.1793071442132473 No Hit GTATCAACGCAGAGTACTTTTTTTT 955 0.15496680789470696 No Hit GTCCTTCAGTGTGCATTTCTCATTT 668 0.10839563107189973 No Hit CTGAAGGACCTGGAATATGGCGAGA 668 0.10839563107189973 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.6226890879026906E-4 0.0 0.0 0.0 0.0 11 1.6226890879026906E-4 0.0 0.0 0.0 0.0 12 1.6226890879026906E-4 0.0 0.0 0.0 0.0 13 1.6226890879026906E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAATAC 50 8.5028994E-5 15.240942 3 TAGGACC 340 0.0 14.2814 4 CGGTTTC 40 0.0053341757 14.2257805 13 GTTCCAA 65 5.2699543E-5 13.1989565 1 GGCGAGG 445 0.0 13.00034 19 CTAAGAC 60 3.9940837E-4 12.700785 3 AGGGGAG 75 1.4457559E-5 12.691477 5 TCCAACG 75 1.5011801E-5 12.645139 18 CCTATAC 55 0.0030036792 12.123476 3 CCAACGA 55 0.0031041258 12.07036 19 ACTGATC 65 7.9294655E-4 11.70663 8 GTCCTAA 515 0.0 11.661215 1 GTGTAGG 100 1.845523E-6 11.439096 1 ATACCTG 75 2.0326416E-4 11.422329 5 GACGTGA 535 0.0 11.386745 7 GTCCTAC 1105 0.0 11.301068 1 TGGCGAG 985 0.0 11.265085 18 TGTAGGA 1160 0.0 11.250049 2 GTAGGAC 1120 0.0 11.226587 3 CCTACAC 120 1.2231067E-7 11.113187 3 >>END_MODULE