FastQCFastQC Report
Thu 26 May 2016
SRR1926949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23350.37889790202527823No Hit
CTGTAGGACGTGGAATATGGCAAGA22040.357640674973753No Hit
GTCCTAAAGTGTGTATTTCTCATTT18460.29954840562683666No Hit
CTTTAGGACGTGAAATATGGCGAGG17600.28559327947087354No Hit
GTCCTACAGTGTGCATTTCTCATTT17280.28040067438958494No Hit
GTATCAACGCAGAGTACTTTTTTTT14750.23934664046564685No Hit
CTGTAGGACCTGGAATATGGCGAGA13810.2240933630393616No Hit
GGTATCAACGCAGAGTACTTTTTTT9390.15237050535406266No Hit
CTGAAGGACCTGGAATATGGCGAGA9320.15123462299253077No Hit
TATCAACGCAGAGTACTTTTTTTTT7590.1231621017718142No Hit
GTACATGGGAAGCAGTGGTATCAAC6430.104338908352143No Hit
GAATATGGCAAGAAAACTGAAAATC6340.10287848817303058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG400.005179737714.2889081
TAGGACC4900.013.9847694
GGACTAT551.926953E-413.8435096
CCAACGA500.001509228313.2865419
GGACCTG7900.012.2883056
TCCAACG853.9771858E-612.28167418
CTGTAGG13450.012.2526941
TGTAGGA13350.012.1294212
GTCATAC550.003002857612.1239211
TCCTAGA1103.659443E-812.1229342
GGTAGCA550.003004690212.1229342
CTAGACT550.00302307112.113074
CAAGACC550.00302307112.113074
CCGTGTG550.003086288212.0796529
ATCCCGT550.003086288212.07965210
AGGACCT8450.011.9390025
GTCCTAG802.781051E-511.9074231
TAGCACT802.8066652E-511.8967664
GTAGGAC13500.011.8448553
CTCTTAC657.830118E-411.7242321