##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926949_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616261 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.714917867591815 31.0 30.0 33.0 25.0 34.0 2 29.990611120937395 31.0 30.0 33.0 26.0 34.0 3 30.090588241021255 31.0 30.0 34.0 26.0 34.0 4 33.674399321066886 35.0 35.0 37.0 28.0 37.0 5 33.647198183886374 35.0 35.0 37.0 28.0 37.0 6 33.623266765217984 37.0 35.0 37.0 29.0 37.0 7 33.611776503786544 37.0 35.0 37.0 28.0 37.0 8 33.63318950899051 37.0 35.0 37.0 28.0 37.0 9 35.14278041284456 39.0 35.0 39.0 29.0 39.0 10 35.02813093802788 39.0 35.0 39.0 28.0 39.0 11 35.07684081906854 39.0 35.0 39.0 29.0 39.0 12 34.96250776862401 39.0 35.0 39.0 28.0 39.0 13 34.980586147752334 39.0 35.0 39.0 28.0 39.0 14 36.03162296494505 40.0 36.0 41.0 27.0 41.0 15 36.03906461710217 40.0 36.0 41.0 27.0 41.0 16 36.02859015903976 40.0 36.0 41.0 27.0 41.0 17 36.00479017818749 40.0 36.0 41.0 27.0 41.0 18 35.98308995701497 40.0 36.0 41.0 27.0 41.0 19 36.041128677621984 40.0 36.0 41.0 27.0 41.0 20 36.006953222741664 40.0 36.0 41.0 27.0 41.0 21 35.94859483238433 40.0 36.0 41.0 27.0 41.0 22 35.863423127538496 40.0 36.0 41.0 27.0 41.0 23 35.79033883370845 40.0 36.0 41.0 27.0 41.0 24 35.72682516011884 40.0 36.0 41.0 27.0 41.0 25 35.606470310469106 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2180745343700572 1101 2 0.12261105650575388 1101 3 0.29285172515017166 1101 4 0.4409686343187005 1101 5 0.3622289065501789 1101 6 0.47888979028158474 1101 7 0.24019451352968701 1101 8 0.37240164256471076 1101 9 0.3213214514862699 1101 10 0.19322912903849954 1101 11 0.25329148389327116 1101 12 0.3661117985851092 1101 13 0.43252893368619283 1101 14 0.5850372723190986 1101 15 0.5908167700323261 1101 16 0.3421120294461062 1101 17 0.3188482501617571 1101 18 0.1810833098942055 1101 19 0.13787256727367492 1101 20 0.10538947299917112 1101 21 0.17923896506493975 1101 22 0.07330964726304501 1101 23 0.14247764101935445 1101 24 0.30710513255870353 1101 25 -0.018443465867377995 1102 1 -1.2149119803939534 1102 2 -1.0073354675584199 1102 3 -0.7577876610390888 1102 4 -0.9800439209010179 1102 5 -1.1361842531801187 1102 6 -0.9687212304112762 1102 7 -0.9278884904228804 1102 8 -0.9865846396952236 1102 9 -1.183450694526286 1102 10 -1.0530217428517616 1102 11 -1.051579476348536 1102 12 -0.8671362832563219 1102 13 -0.9187265882854376 1102 14 -1.0435200399338669 1102 15 -0.6901992727484227 1102 16 -0.990159535306276 1102 17 -0.9005484017796519 1102 18 -0.9523624004057183 1102 19 -1.0812392671834345 1102 20 -0.8925819290003645 1102 21 -0.9514050712056914 1102 22 -0.9173843773823904 1102 23 -0.8843707663277769 1102 24 -1.0510731980620776 1102 25 -1.01099171046166 1103 1 -0.7385206157672926 1103 2 -0.857563235433247 1103 3 -0.6464586850042302 1103 4 -0.8071582009564722 1103 5 -0.8196859366818003 1103 6 -0.7953997806192419 1103 7 -0.747001186006166 1103 8 -0.6220570104617167 1103 9 -0.7746172621634457 1103 10 -0.7049924301165689 1103 11 -0.949678109741285 1103 12 -1.0351101394654734 1103 13 -0.9870173389291281 1103 14 -1.103096193627664 1103 15 -1.0548259314927293 1103 16 -0.9577174675700917 1103 17 -1.0235430541860708 1103 18 -1.1006491898689816 1103 19 -0.9608391879598202 1103 20 -0.9988802058868771 1103 21 -0.7082480104015758 1103 22 -0.8625220284023953 1103 23 -0.9949075062762844 1103 24 -1.0359018770084063 1103 25 -1.1674583375165213 1104 1 -0.36565868001627067 1104 2 -0.4547107577491083 1104 3 -0.4189615906541313 1104 4 -0.2681660262128034 1104 5 -0.33000275839488324 1104 6 -0.6394757839507363 1104 7 -0.5095495938982424 1104 8 -0.49397078677137785 1104 9 -0.7680789616980661 1104 10 -0.5435935773189087 1104 11 -0.5469479752108697 1104 12 -0.6350056772342185 1104 13 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1107 11 0.16000584823008523 1107 12 0.27909106844384723 1107 13 0.3586984438678158 1107 14 0.4150913908294669 1107 15 0.23301086285933081 1107 16 0.3615304847754004 1107 17 0.3403809964468394 1107 18 0.2726720092154338 1107 19 0.469774970502435 1107 20 0.43036195511254505 1107 21 0.5114991008198473 1107 22 0.5493736722125959 1107 23 0.5187159459175348 1107 24 0.49626767154237683 1107 25 0.2981589923971626 1108 1 0.5759314783633762 1108 2 0.3353111632293384 1108 3 0.7132776914626398 1108 4 0.48593522519615107 1108 5 0.6556990471469035 1108 6 0.6435445626819387 1108 7 0.528442158650563 1108 8 0.6106736485041111 1108 9 0.6416093731604349 1108 10 0.6748970330800717 1108 11 0.5086508639684411 1108 12 0.5221788024364358 1108 13 0.3948763885606823 1108 14 0.31716687187364556 1108 15 0.4104005480472708 1108 16 0.5721755995820601 1108 17 0.5175455263902364 1108 18 0.4530776489039994 1108 19 0.6968350284123659 1108 20 0.7325146644056062 1108 21 0.6280766060096781 1108 22 0.7177008588077527 1108 23 0.5576497908035805 1108 24 0.4511645729559035 1108 25 0.391122215539518 1109 1 0.5572750594913245 1109 2 0.4821811690953126 1109 3 0.48187758069731856 1109 4 0.38645726410820913 1109 5 0.5545912847264987 1109 6 0.45456844276701247 1109 7 0.3302672739764887 1109 8 0.4067125321557441 1109 9 0.5839054486381556 1109 10 0.7793812807006404 1109 11 0.590848779963757 1109 12 0.4517510669754756 1109 13 0.6890172964648897 1109 14 0.4367628297041719 1109 15 0.5160470668593504 1109 16 0.6107293584479336 1109 17 0.3920147759060484 1109 18 0.3422577124575099 1109 19 0.421491453838577 1109 20 0.44316594044610014 1109 21 0.6109910010223985 1109 22 0.5386772321502278 1109 23 0.7416665956720152 1109 24 0.7703522065652066 1109 25 0.635267984446358 1110 1 0.46570299286808137 1110 2 0.30360279204294116 1110 3 0.38571015130899156 1110 4 0.7599050338875131 1110 5 0.6917204624251312 1110 6 0.7848276271482746 1110 7 0.8888470467672036 1110 8 0.664667183348044 1110 9 0.759050520835892 1110 10 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0.178917808688297 2214 13 -0.010627665769789019 2214 14 0.08328324615442995 2214 15 -0.1445854146747223 2214 16 0.09890577755715668 2214 17 -0.029501346142374985 2214 18 0.09262642115883324 2214 19 0.12829612411491098 2214 20 0.19853851665013877 2214 21 0.15691264064026456 2214 22 0.07238244204737754 2214 23 0.12281635696425752 2214 24 -0.039668538699011435 2214 25 0.10033594780215083 2215 1 0.26762939979448674 2215 2 0.13499200888670515 2215 3 0.12680617649592563 2215 4 0.22356697800400127 2215 5 0.10114195002844184 2215 6 0.19018378614079978 2215 7 -0.11793178046617214 2215 8 -0.06821947544771234 2215 9 -0.1313804118677666 2215 10 -0.024979980692350523 2215 11 -0.2560459902268164 2215 12 -0.1787329219117879 2215 13 -0.20811288825997565 2215 14 -0.14360662002044222 2215 15 -0.18204037282481522 2215 16 -0.05138693535720762 2215 17 -0.07878114942416659 2215 18 -0.020842155944301055 2215 19 -0.1515270186476556 2215 20 -0.15768506116231862 2215 21 -0.09852501009034142 2215 22 -0.16852265087360507 2215 23 -0.0015492740945219907 2215 24 -0.03934414280361409 2215 25 -0.2815697598632383 2216 1 0.33819323268960844 2216 2 0.25471739597405474 2216 3 -1.5309501775794452 2216 4 -0.8160532381806362 2216 5 -0.13476807042145822 2216 6 0.17330385625473355 2216 7 0.10944186837986791 2216 8 0.18475160255404433 2216 9 0.2171092171126574 2216 10 0.23357099798132452 2216 11 0.08913659814595576 2216 12 0.26420017770843174 2216 13 0.0012005794583984652 2216 14 0.13563965517675314 2216 15 0.15247321175012019 2216 16 0.33399430916514206 2216 17 0.34046512577302934 2216 18 0.46767084434784323 2216 19 0.19810196177887462 2216 20 -0.013355525667137158 2216 21 0.21980978394997663 2216 22 0.20696084313749452 2216 23 0.3116672026802405 2216 24 0.10519573449700204 2216 25 0.16874867425887885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16223.0 3 18.0 4 269.0 5 1666.0 6 2259.0 7 1658.0 8 1372.0 9 1120.0 10 980.0 11 940.0 12 829.0 13 823.0 14 798.0 15 799.0 16 866.0 17 857.0 18 955.0 19 1208.0 20 1484.0 21 2081.0 22 2439.0 23 2954.0 24 3653.0 25 4179.0 26 5023.0 27 5592.0 28 6512.0 29 7961.0 30 10116.0 31 13005.0 32 16846.0 33 22131.0 34 30090.0 35 41357.0 36 61387.0 37 105949.0 38 239862.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.984011358725844 20.04997059597571 10.296769456204249 28.66924858909419 2 13.382018055361547 20.98976386393833 42.06788789952929 23.560330181170837 3 18.592724233350374 25.316843975947613 29.240532008991245 26.849899781710775 4 11.617967062656893 17.782562907599214 38.96044046272602 31.63902956701787 5 12.144044087577974 39.504858591300625 35.964448223709404 12.386649097411997 6 30.86184941165742 38.90730795410505 18.066171848792862 12.164670785444669 7 27.59302899284592 31.90478972721017 22.243212713745958 18.25896856619795 8 23.78931549932571 40.46617905521088 19.78623858124216 15.958266864221251 9 26.159201557935734 15.781239857189226 20.2963323596235 37.763226225251536 10 14.383311561336178 30.33923746622636 34.71597685947731 20.561474112960155 11 33.50253312864918 22.77040864346985 25.70370995433553 18.02334827354544 12 23.88703450984083 25.15549468648734 32.34013620896409 18.617334594707742 13 29.372323559813516 23.215638929862052 25.528797238814327 21.8832402715101 14 19.57436796157466 24.284068412682956 27.647097004511085 28.494466621231297 15 21.527963715729946 34.337154865353874 22.779738578000632 21.355142840915544 16 20.292994643468553 29.155855430445417 30.27194822939131 20.279201696694724 17 20.01100183201598 30.742493845951635 28.940984420562067 20.30551990147032 18 20.001687604866795 28.208477618140453 32.19252326136131 19.59731151563144 19 23.083601077467303 27.421056047772048 27.777399149709538 21.717943725051114 20 23.060546266486657 28.633392616055666 28.90503297330979 19.401028144147887 21 21.348050194428755 27.76647126414396 27.38264488025915 23.50283366116813 22 22.226459243729522 30.42152594436448 27.452653989137723 19.899360822768276 23 20.441663515945354 29.90112955387409 28.794617864833246 20.86258906534731 24 22.675457314352197 27.483647350716662 29.84693173833814 19.993963596593 25 21.270183865889873 29.12903068759027 29.137793933886176 20.46299151263368 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 226.0 1 226.0 2 241.5 3 257.0 4 257.0 5 257.0 6 413.5 7 570.0 8 570.0 9 570.0 10 940.5 11 1311.0 12 1311.0 13 1311.0 14 2090.0 15 2869.0 16 2869.0 17 2869.0 18 5144.5 19 7420.0 20 7420.0 21 7420.0 22 12175.5 23 16931.0 24 16931.0 25 16931.0 26 25001.5 27 33072.0 28 33072.0 29 33072.0 30 44025.5 31 54979.0 32 54979.0 33 54979.0 34 62221.0 35 69463.0 36 69463.0 37 69463.0 38 73046.5 39 76630.0 40 76630.0 41 76630.0 42 78898.0 43 81166.0 44 81166.0 45 81166.0 46 82194.0 47 83222.0 48 83222.0 49 83222.0 50 77500.0 51 71778.0 52 71778.0 53 71778.0 54 60273.0 55 48768.0 56 48768.0 57 48768.0 58 40501.0 59 32234.0 60 32234.0 61 32234.0 62 25612.5 63 18991.0 64 18991.0 65 18991.0 66 14339.5 67 9688.0 68 9688.0 69 9688.0 70 6983.0 71 4278.0 72 4278.0 73 4278.0 74 2943.5 75 1609.0 76 1609.0 77 1609.0 78 1095.0 79 581.0 80 581.0 81 581.0 82 370.5 83 160.0 84 160.0 85 160.0 86 103.5 87 47.0 88 47.0 89 47.0 90 28.0 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11342596724439807 2 0.09719907636537116 3 0.01720050433176852 4 0.0 5 0.005354873990078879 6 0.35098764971335195 7 0.017687311058139327 8 0.009736134527416144 9 0.009898403436206413 10 0.004218991628546996 11 0.004381260537337265 12 3.245378175805381E-4 13 6.490756351610762E-4 14 1.6226890879026906E-4 15 0.002596302540644305 16 6.490756351610762E-4 17 0.0 18 4.8680672637080714E-4 19 1.6226890879026906E-4 20 8.113445439513452E-4 21 0.015740084152656098 22 0.0 23 0.0 24 0.0 25 0.008275714348303721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 616261.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.02875296173045 #Duplication Level Percentage of deduplicated Percentage of total 1 92.80084358918533 75.19536629828283 2 5.438435236262505 8.813392505149691 3 0.8203504030676838 1.9941591045668199 4 0.30867627468575637 1.000466144266376 5 0.14657234973906166 0.5938287359013391 6 0.0907919675986193 0.44140559480747743 7 0.05955582230180624 0.3378013808908042 8 0.04408498889142831 0.285772133936334 9 0.03576168323226288 0.2607952136510418 >10 0.20698115111195795 3.145193198282746 >50 0.024474870531593546 1.3695222510706624 >100 0.020261863270136706 3.483528217082636 >500 0.0018055125685323072 1.0096217338190412 >1k 0.0014042875533029056 2.069147488292155 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2335 0.37889790202527823 No Hit CTGTAGGACGTGGAATATGGCAAGA 2204 0.357640674973753 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1846 0.29954840562683666 No Hit CTTTAGGACGTGAAATATGGCGAGG 1760 0.28559327947087354 No Hit GTCCTACAGTGTGCATTTCTCATTT 1728 0.28040067438958494 No Hit GTATCAACGCAGAGTACTTTTTTTT 1475 0.23934664046564685 No Hit CTGTAGGACCTGGAATATGGCGAGA 1381 0.2240933630393616 No Hit GGTATCAACGCAGAGTACTTTTTTT 939 0.15237050535406266 No Hit CTGAAGGACCTGGAATATGGCGAGA 932 0.15123462299253077 No Hit TATCAACGCAGAGTACTTTTTTTTT 759 0.1231621017718142 No Hit GTACATGGGAAGCAGTGGTATCAAC 643 0.104338908352143 No Hit GAATATGGCAAGAAAACTGAAAATC 634 0.10287848817303058 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.6226890879026906E-4 0.0 9 0.0 0.0 0.0 1.6226890879026906E-4 0.0 10 0.0 0.0 0.0 1.6226890879026906E-4 0.0 11 0.0 0.0 0.0 1.6226890879026906E-4 0.0 12 0.0 0.0 0.0 1.6226890879026906E-4 0.0 13 0.0 0.0 0.0 1.6226890879026906E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 40 0.0051797377 14.288908 1 TAGGACC 490 0.0 13.984769 4 GGACTAT 55 1.926953E-4 13.843509 6 CCAACGA 50 0.0015092283 13.28654 19 GGACCTG 790 0.0 12.288305 6 TCCAACG 85 3.9771858E-6 12.281674 18 CTGTAGG 1345 0.0 12.252694 1 TGTAGGA 1335 0.0 12.129421 2 GTCATAC 55 0.0030028576 12.123921 1 TCCTAGA 110 3.659443E-8 12.122934 2 GGTAGCA 55 0.0030046902 12.122934 2 CTAGACT 55 0.003023071 12.11307 4 CAAGACC 55 0.003023071 12.11307 4 CCGTGTG 55 0.0030862882 12.079652 9 ATCCCGT 55 0.0030862882 12.079652 10 AGGACCT 845 0.0 11.939002 5 GTCCTAG 80 2.781051E-5 11.907423 1 TAGCACT 80 2.8066652E-5 11.896766 4 GTAGGAC 1350 0.0 11.844855 3 CTCTTAC 65 7.830118E-4 11.724232 1 >>END_MODULE