FastQCFastQC Report
Thu 26 May 2016
SRR1926948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722822
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12490.17279496196850677No Hit
CTGTAGGACGTGGAATATGGCAAGA10080.13945342006745784No Hit
GTCCTACAGTGTGCATTTCTCATTT9590.13267443436973417No Hit
GTCCTAAAGTGTGTATTTCTCATTT8570.11856307638671762No Hit
GTATCAACGCAGAGTACTTTTTTTT8500.11759464985847139No Hit
GTACATGGGAAGCAGTGGTATCAAC7410.10251486534720858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGT250.006086029618.96906510
GCGCCAC350.002195462816.25582313
TAGGACC2500.015.6162354
CGGTTTC500.001519076113.2755913
GTCTTTA603.954236E-412.7164461
TCCTATA1001.3802855E-712.38905052
GTGCTAG853.766152E-612.3424331
GTTTTAG853.766152E-612.3424331
CCACGTC1251.4388206E-912.1410429
CCGGTTT550.003109793212.067883512
GGGCTAT802.7487518E-511.9216681
AGGACCT4400.011.899315
GTCTAGG657.7547826E-411.7382571
GTGTAGG1655.456968E-1211.5604051
GTATTAG751.996769E-411.4448011
CCTAGAC1001.852919E-611.4360473
GTATAAA1803.6379788E-1211.126891
GGACCTA600.00578177911.1060246
GGCGTGC600.00581775711.0967748
CCTGATC600.00581775711.0967748
Produced by FastQC (version 0.11.5)