##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926948_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 722822 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.470045184014875 31.0 28.0 31.0 16.0 33.0 2 28.068177227588535 31.0 28.0 31.0 16.0 34.0 3 28.010344178788138 31.0 28.0 31.0 16.0 34.0 4 31.77920705235867 35.0 32.0 37.0 19.0 37.0 5 31.567655660729752 35.0 32.0 37.0 18.0 37.0 6 31.24801541735033 35.0 32.0 37.0 17.0 37.0 7 31.347681725238026 35.0 32.0 37.0 17.0 37.0 8 31.16774530935694 35.0 32.0 37.0 11.0 37.0 9 33.17814482680383 37.0 33.0 39.0 17.0 39.0 10 33.272854174333375 37.0 33.0 39.0 17.0 39.0 11 33.4539554689813 38.0 34.0 39.0 18.0 39.0 12 33.299088848983565 38.0 34.0 39.0 17.0 39.0 13 33.42204719834206 38.0 34.0 39.0 17.0 39.0 14 34.455311266120844 39.0 34.0 41.0 17.0 41.0 15 34.01903373167944 39.0 33.0 41.0 11.0 41.0 16 34.10555849157884 39.0 33.0 41.0 17.0 41.0 17 34.33703733422613 39.0 34.0 41.0 17.0 41.0 18 34.39733433680768 39.0 34.0 41.0 17.0 41.0 19 33.76874389545421 39.0 33.0 41.0 10.0 41.0 20 34.01786885291261 39.0 34.0 41.0 10.0 41.0 21 34.020960900470655 39.0 34.0 41.0 10.0 41.0 22 34.040762456040355 39.0 34.0 41.0 10.0 41.0 23 34.04378394680848 39.0 34.0 41.0 2.0 41.0 24 32.73991937157419 39.0 32.0 41.0 2.0 41.0 25 33.32226744620391 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6783048134183325 1101 2 -0.5337246790553891 1101 3 -1.1863548434535147 1101 4 -0.5192702748981262 1101 5 -0.4178618134417853 1101 6 -2.3902832809454075 1101 7 -0.7111973901347746 1101 8 -0.22659313929643332 1101 9 -0.40655642278713344 1101 10 -9.485869328290757 1101 11 -4.189124933798066 1101 12 -1.7873975807986646 1101 13 -0.8337689912509987 1101 14 -0.7696111436080386 1101 15 -2.178863981504211 1101 16 -0.6120655651579057 1101 17 -0.5584539008061569 1101 18 -0.2577486251918941 1101 19 -1.048569774017892 1101 20 -0.48226957488962796 1101 21 -0.4581742138499507 1101 22 -0.23829698598109417 1101 23 -0.3734327310387968 1101 24 -0.480014285943426 1101 25 -0.4660431016033755 1102 1 -0.6691142101363496 1102 2 -0.5688936667149278 1102 3 -0.7536513051505267 1102 4 -0.4974238744189563 1102 5 -0.380173645660868 1102 6 -0.9002578248945312 1102 7 -0.2739487576267834 1102 8 0.2804094767752332 1102 9 0.12000771501026009 1102 10 0.0472922427615714 1102 11 -0.17006724809661478 1102 12 -0.2732584621593759 1102 13 -0.330834939158585 1102 14 -0.6853692318498119 1102 15 0.0923838934880834 1102 16 -0.06603151218465797 1102 17 -0.2872923473704674 1102 18 -0.6378937863401504 1102 19 -0.16055878123191292 1102 20 -0.14373228485800382 1102 21 -0.28484757484141454 1102 22 -0.39714714761380776 1102 23 -0.6704141190541932 1102 24 -4.070400002368306 1102 25 -2.1591312112493704 1103 1 -0.20731810267875161 1103 2 -0.279130893478154 1103 3 0.25847894325864473 1103 4 0.24077824313831186 1103 5 0.002840634094539496 1103 6 0.5492411855512565 1103 7 0.5348880058653229 1103 8 0.9195904986341716 1103 9 0.5304251027646387 1103 10 0.600649183397195 1103 11 0.0654794916994419 1103 12 0.11568870540809684 1103 13 0.19363944336846117 1103 14 0.3312783537266597 1103 15 0.5905423464110413 1103 16 0.42783058922024253 1103 17 0.2802005748763037 1103 18 0.17246236442709062 1103 19 -1.0685568642479666 1103 20 -0.0017991485480237657 1103 21 0.23912537438513937 1103 22 0.04582080502959229 1103 23 0.09983624259902513 1103 24 1.6966527235651157 1103 25 1.0281811950092248 1104 1 -0.29505188748247946 1104 2 -0.12728762017930606 1104 3 -1.2152568357701412 1104 4 -0.32231682184711374 1104 5 -0.18396562832608865 1104 6 -1.2564049641402626 1104 7 -0.14564302579992372 1104 8 0.3962068249669457 1104 9 0.37772839808305747 1104 10 0.35365090757094464 1104 11 0.3029013326184469 1104 12 0.31652898044497135 1104 13 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11 -2.5801114878943743 2216 12 -2.25581534189935 2216 13 -2.0643037004442455 2216 14 -2.0096830635580076 2216 15 -2.9007995662446753 2216 16 -2.1447422380784786 2216 17 -2.4144185662220394 2216 18 -2.2140494382000284 2216 19 -1.6015911936551674 2216 20 -2.7264065354900033 2216 21 -5.06386527076052 2216 22 -3.5981312574007305 2216 23 -3.4600493664359213 2216 24 -3.893905780627602 2216 25 -3.265507610671545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42024.0 3 3.0 4 330.0 5 1941.0 6 2399.0 7 2088.0 8 1633.0 9 1556.0 10 1438.0 11 1373.0 12 1276.0 13 1322.0 14 1275.0 15 1423.0 16 1469.0 17 1657.0 18 1787.0 19 2299.0 20 2900.0 21 3866.0 22 4749.0 23 5679.0 24 6510.0 25 7688.0 26 8645.0 27 9459.0 28 10409.0 29 12447.0 30 14739.0 31 18105.0 32 23035.0 33 30309.0 34 41053.0 35 56926.0 36 83516.0 37 137108.0 38 178386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18073739069673 18.597535191252135 12.464767154738913 25.75696026331223 2 14.201627705627706 19.955670995670996 42.48588744588744 23.356813852813854 3 18.917391310371006 24.36977816690194 29.54121983960634 27.171610683120704 4 11.283233313260101 16.57606024738529 39.28774632971323 32.85296010964138 5 12.60523621066615 38.11654166551352 36.67367780145573 12.604544322364596 6 31.696108575745917 37.59440737491455 18.55642855759092 12.153055491748608 7 27.7455043034897 31.616563369961337 22.631276558540506 18.006655768008457 8 24.27484496398335 37.82573521555175 21.373114694042368 16.52630512642252 9 25.831934610333988 15.921918738921493 20.47495236500651 37.771194285738005 10 13.722667943627354 29.897487921234404 35.49558576650165 20.88425836863659 11 34.07069176646396 23.094580851372946 24.57109649031776 18.263630891845335 12 22.59488390048969 25.32340573323057 32.37087639529043 19.710833970989306 13 28.508313742962343 22.449389043321126 25.957944927419923 23.08435228629661 14 19.706712162534266 22.893221696879632 28.35521645821312 29.04484968237298 15 22.66636041747668 31.686967205969648 23.346288965481936 22.30038341107174 16 20.975011035328272 29.256557890847805 28.633598042837832 21.134833030986094 17 20.80191727330969 29.407922866279318 28.541026410937974 21.249133449473014 18 20.652864237059013 28.574017984760413 30.137134144063495 20.635983634117082 19 22.989126072793596 27.765046786393338 27.847383089368794 21.398444051444276 20 22.502220832376278 28.310977415310873 28.698550716616257 20.488251035696596 21 22.25616800950851 27.715397781439865 27.65479348185538 22.373640727196246 22 21.88495396442794 29.056928028426654 28.017080345702656 21.04103766144275 23 21.221153074468585 28.648778044372857 28.350311473459026 21.779757407699524 24 22.364663297892946 27.728180917538843 28.85545636183508 21.051699422733133 25 21.632474032092695 28.17346714283145 28.616101140277205 21.57795768479866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 253.5 3 322.0 4 322.0 5 322.0 6 489.0 7 656.0 8 656.0 9 656.0 10 1084.0 11 1512.0 12 1512.0 13 1512.0 14 2549.5 15 3587.0 16 3587.0 17 3587.0 18 6335.5 19 9084.0 20 9084.0 21 9084.0 22 14223.0 23 19362.0 24 19362.0 25 19362.0 26 27590.0 27 35818.0 28 35818.0 29 35818.0 30 46666.0 31 57514.0 32 57514.0 33 57514.0 34 66630.5 35 75747.0 36 75747.0 37 75747.0 38 82121.0 39 88495.0 40 88495.0 41 88495.0 42 91733.0 43 94971.0 44 94971.0 45 94971.0 46 96427.0 47 97883.0 48 97883.0 49 97883.0 50 91754.5 51 85626.0 52 85626.0 53 85626.0 54 74137.0 55 62648.0 56 62648.0 57 62648.0 58 52448.5 59 42249.0 60 42249.0 61 42249.0 62 33636.0 63 25023.0 64 25023.0 65 25023.0 66 18971.5 67 12920.0 68 12920.0 69 12920.0 70 9300.0 71 5680.0 72 5680.0 73 5680.0 74 3985.0 75 2290.0 76 2290.0 77 2290.0 78 1561.5 79 833.0 80 833.0 81 833.0 82 569.0 83 305.0 84 305.0 85 305.0 86 192.0 87 79.0 88 79.0 89 79.0 90 49.0 91 19.0 92 19.0 93 19.0 94 12.5 95 6.0 96 6.0 97 6.0 98 7.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21429895603620255 2 0.13101427460702636 3 0.13461128742622666 4 0.0646078840987131 5 0.02241215679655572 6 0.021582076915201805 7 0.31044987562636445 8 0.30201073016593294 9 0.019230183918032377 10 0.03776863460160316 11 0.014803091217478161 12 0.01701663756775527 13 0.014249704629908886 14 0.016463250980185994 15 0.013696318042339607 16 0.019783570505601655 17 0.017846717449109185 18 0.013696318042339607 19 0.024349009853048192 20 0.016601597627078312 21 0.013973011336124247 22 0.016601597627078312 23 0.017708370802216867 24 0.014664744570585841 25 0.014249704629908886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 722822.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.6840183786859 #Duplication Level Percentage of deduplicated Percentage of total 1 93.88402385144799 82.32128472855351 2 4.807161077857906 8.430224006003922 3 0.6820777600306607 1.794219565386641 4 0.22349827825999977 0.783889085542179 5 0.10869112066099246 0.47652371108192143 6 0.06130487582117722 0.3225274714924296 7 0.042748607033681066 0.26238587513631584 8 0.031773487966079435 0.22288216822181317 9 0.02031244738913217 0.16029693091662797 >10 0.11647751773106547 1.927694905565831 >50 0.011202513325020232 0.6629636723087731 >100 0.009150747412155744 1.5189775593301142 >500 0.0012621720513602112 0.8038960451404025 >1k 3.155430128400528E-4 0.31223427531954717 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1249 0.17279496196850677 No Hit CTGTAGGACGTGGAATATGGCAAGA 1008 0.13945342006745784 No Hit GTCCTACAGTGTGCATTTCTCATTT 959 0.13267443436973417 No Hit GTCCTAAAGTGTGTATTTCTCATTT 857 0.11856307638671762 No Hit GTATCAACGCAGAGTACTTTTTTTT 850 0.11759464985847139 No Hit GTACATGGGAAGCAGTGGTATCAAC 741 0.10251486534720858 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.3834664689231927E-4 0.0 3 0.0 0.0 0.0 1.3834664689231927E-4 0.0 4 0.0 0.0 0.0 1.3834664689231927E-4 0.0 5 0.0 0.0 0.0 1.3834664689231927E-4 0.0 6 0.0 0.0 0.0 1.3834664689231927E-4 0.0 7 0.0 0.0 0.0 1.3834664689231927E-4 0.0 8 0.0 0.0 0.0 1.3834664689231927E-4 0.0 9 0.0 0.0 0.0 1.3834664689231927E-4 0.0 10 0.0 0.0 0.0 1.3834664689231927E-4 0.0 11 0.0 0.0 0.0 1.3834664689231927E-4 0.0 12 0.0 0.0 0.0 1.3834664689231927E-4 1.3834664689231927E-4 13 0.0 0.0 0.0 1.3834664689231927E-4 1.3834664689231927E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGGT 25 0.0060860296 18.969065 10 GCGCCAC 35 0.0021954628 16.255823 13 TAGGACC 250 0.0 15.616235 4 CGGTTTC 50 0.0015190761 13.27559 13 GTCTTTA 60 3.954236E-4 12.716446 1 TCCTATA 100 1.3802855E-7 12.3890505 2 GTGCTAG 85 3.766152E-6 12.342433 1 GTTTTAG 85 3.766152E-6 12.342433 1 CCACGTC 125 1.4388206E-9 12.141042 9 CCGGTTT 55 0.0031097932 12.0678835 12 GGGCTAT 80 2.7487518E-5 11.921668 1 AGGACCT 440 0.0 11.89931 5 GTCTAGG 65 7.7547826E-4 11.738257 1 GTGTAGG 165 5.456968E-12 11.560405 1 GTATTAG 75 1.996769E-4 11.444801 1 CCTAGAC 100 1.852919E-6 11.436047 3 GTATAAA 180 3.6379788E-12 11.12689 1 GGACCTA 60 0.005781779 11.106024 6 GGCGTGC 60 0.005817757 11.096774 8 CCTGATC 60 0.005817757 11.096774 8 >>END_MODULE