FastQCFastQC Report
Thu 26 May 2016
SRR1926948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722822
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14760.20419965081306327No Hit
GTCCTACAGTGGACATTTCTAAATT12240.1693362957961988No Hit
CTGTAGGACGTGGAATATGGCAAGA12000.16601597627078313No Hit
GTCCTACAGTGTGCATTTCTCATTT9680.13391955419176504No Hit
GGTATCAACGCAGAGTACTTTTTTT9280.12838568831607228No Hit
GTCCTAAAGTGTGTATTTCTCATTT9230.1276939550816107No Hit
CTTTAGGACGTGAAATATGGCGAGG9130.12631048861268748No Hit
GTACATGGGAAGCAGTGGTATCAAC8160.11289086386413252No Hit
CTGTAGGACCTGGAATATGGCGAGA7770.10749534463533206No Hit
TATCAACGCAGAGTACTTTTTTTTT7310.10113139887828537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC350.00218702716.26519813
TAGGACC3100.015.0492674
GTCCTAT1204.9112714E-1113.503971
CTAGGTA500.001473503413.329354
TTAGGAC3100.012.5922433
TAGGACT1301.891749E-1012.4504934
GCCTAAC550.002988936812.1319091
GATTAGG550.002988936812.1319091
TGCGCCA550.003094793512.07567712
GTGCGCC550.003094793512.07567711
ACCTATA802.7639142E-511.9152682
CTGGACT802.7975326E-511.9012064
TGTAGGA7650.011.8373892
GTACAGT1156.8368536E-811.5907396
GTAGGAC7750.011.548013
GTATTAA752.0067615E-411.4386581
GATTAAA1501.6552804E-1011.4386581
GTCCTAA2700.011.2974391
TCCTATA1354.4638E-911.2974392
TATAGGA1354.4638E-911.2974392
Produced by FastQC (version 0.11.5)