##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 722822 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88525390760104 31.0 30.0 33.0 26.0 34.0 2 30.219394263041245 31.0 30.0 34.0 26.0 34.0 3 30.266973058373985 31.0 30.0 34.0 26.0 34.0 4 33.86373685360988 35.0 35.0 37.0 30.0 37.0 5 33.801389553721386 37.0 35.0 37.0 30.0 37.0 6 33.71578479902383 37.0 35.0 37.0 29.0 37.0 7 33.70638276090102 37.0 35.0 37.0 29.0 37.0 8 33.73163655782475 37.0 35.0 37.0 29.0 37.0 9 35.291103480524946 39.0 35.0 39.0 30.0 39.0 10 35.21469739437925 39.0 35.0 39.0 29.0 39.0 11 35.21950217342582 39.0 35.0 39.0 29.0 39.0 12 35.12458668939241 39.0 35.0 39.0 29.0 39.0 13 35.12220159319999 39.0 35.0 39.0 29.0 39.0 14 36.23413371480115 40.0 36.0 41.0 29.0 41.0 15 36.20822968863704 40.0 36.0 41.0 29.0 41.0 16 36.194559656457606 40.0 36.0 41.0 29.0 41.0 17 36.17509151630692 40.0 36.0 41.0 29.0 41.0 18 36.15466463389327 40.0 36.0 41.0 29.0 41.0 19 36.22233551275418 40.0 37.0 41.0 29.0 41.0 20 36.18090484240933 40.0 37.0 41.0 28.0 41.0 21 36.130330842171375 40.0 37.0 41.0 28.0 41.0 22 36.04551604682757 40.0 36.0 41.0 28.0 41.0 23 35.9825268184975 40.0 36.0 41.0 28.0 41.0 24 35.90475940134639 40.0 36.0 41.0 27.0 41.0 25 35.78584907487597 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09847458839990253 1101 2 0.06705736778170035 1101 3 0.2792140671593799 1101 4 0.23954907917852353 1101 5 0.21515377733338426 1101 6 0.1356856810007372 1101 7 0.11474230019403109 1101 8 0.16429319267684406 1101 9 0.1924724283092729 1101 10 0.2997351831199282 1101 11 0.10808522881488614 1101 12 0.31193143574523674 1101 13 0.3881382557286912 1101 14 0.31612873047772183 1101 15 0.2425517052619881 1101 16 0.40005032713030175 1101 17 0.41788630932190785 1101 18 0.3393505229334295 1101 19 0.31324595116666387 1101 20 0.29109258159389384 1101 21 0.4017358649260032 1101 22 0.3380017618637723 1101 23 0.37599779001270406 1101 24 0.1961954696986865 1101 25 0.2282659753085241 1102 1 -0.6451648613698033 1102 2 -0.45567779239763695 1102 3 -0.2977380772266365 1102 4 -0.4861888380056314 1102 5 -0.39422444320938865 1102 6 -0.2625817220416593 1102 7 -0.36427803822170546 1102 8 -0.37571684881035594 1102 9 -0.511300176047321 1102 10 -0.2603543094326355 1102 11 -0.5920715593185406 1102 12 -0.22664461090363375 1102 13 -0.3456451828640894 1102 14 -0.499303449846245 1102 15 -0.41616809320360204 1102 16 -0.5045608294904724 1102 17 -0.5064457996546565 1102 18 -0.5437559961057588 1102 19 -0.6392930476794447 1102 20 -0.5723335033711834 1102 21 -0.49071187970312025 1102 22 -0.3198328002304933 1102 23 -0.4940829823009807 1102 24 -0.5182545827108314 1102 25 -0.38674428638672964 1103 1 -0.5708764085355966 1103 2 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>>Per sequence quality scores pass #Quality Count 2 17298.0 3 24.0 4 326.0 5 2009.0 6 2724.0 7 1923.0 8 1534.0 9 1275.0 10 1152.0 11 1086.0 12 997.0 13 919.0 14 913.0 15 891.0 16 927.0 17 974.0 18 1063.0 19 1354.0 20 1681.0 21 2243.0 22 2795.0 23 3408.0 24 4025.0 25 4741.0 26 5852.0 27 6405.0 28 7326.0 29 8850.0 30 11522.0 31 14848.0 32 19271.0 33 25012.0 34 34098.0 35 47976.0 36 70403.0 37 122098.0 38 292879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.706197239669535 19.535189712125096 10.322915728897598 28.435697319307774 2 13.951546109035103 20.93320554122819 40.527364038525995 24.58788431121071 3 18.352691135814563 24.610975970865052 29.467169222339372 27.569163670981006 4 11.59676932910177 17.35973725204822 38.49260260479067 32.55089081405934 5 12.481892705198714 38.784545674656705 35.78762408771748 12.945937532427104 6 31.32088568057194 38.41007843409454 17.926841118900537 12.342194766432984 7 28.097856094142003 31.923897084769592 21.719967951423435 18.25827886966497 8 24.682426028543954 38.6342347577638 19.891109589135862 16.79222962455638 9 25.92281020231398 15.964706435803846 19.978194034352697 38.13428932752948 10 13.785570399229655 30.405842898095166 34.75315166742299 21.055435035252188 11 33.999465950172876 23.27129039443592 24.302310941518776 18.42693271387243 12 22.740547771987178 25.518144929781865 31.946725252103224 19.794582046127733 13 28.51367840867272 22.580926318935056 25.71062148424638 23.194773788145838 14 19.934645044008068 22.965266690831214 27.97867248091508 29.121415784245634 15 22.31035184055318 31.92671821626759 23.30966146881775 22.453268474361476 16 21.305837170178858 29.36459624579423 28.156128253940143 21.17343833008677 17 20.783292129033327 29.614109163956222 28.169906519041366 21.43269218796908 18 20.667581604221255 28.681355472608594 29.96715632427527 20.68390659889488 19 22.48571219074208 27.936731048851787 28.00120085387905 21.576355906527084 20 22.494075263863465 28.217377289134042 28.740746274644895 20.547801172357598 21 22.013169491830293 27.583014980677454 27.76994483424677 22.63387069324548 22 21.950078995935378 29.015857292666798 27.906870571177965 21.12719314021986 23 21.21435152776202 28.691296059057414 28.25536577469972 21.838986638480844 24 21.911095554777738 27.7399245456344 29.051729266305216 21.29725063328265 25 21.658566011004716 28.29833656391078 28.476260444034917 21.566836981049583 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 241.0 1 241.0 2 241.5 3 242.0 4 242.0 5 242.0 6 411.0 7 580.0 8 580.0 9 580.0 10 1003.5 11 1427.0 12 1427.0 13 1427.0 14 2309.0 15 3191.0 16 3191.0 17 3191.0 18 5880.0 19 8569.0 20 8569.0 21 8569.0 22 13594.0 23 18619.0 24 18619.0 25 18619.0 26 26716.0 27 34813.0 28 34813.0 29 34813.0 30 45749.0 31 56685.0 32 56685.0 33 56685.0 34 65720.0 35 74755.0 36 74755.0 37 74755.0 38 80983.0 39 87211.0 40 87211.0 41 87211.0 42 91004.0 43 94797.0 44 94797.0 45 94797.0 46 96797.0 47 98797.0 48 98797.0 49 98797.0 50 93357.0 51 87917.0 52 87917.0 53 87917.0 54 75934.5 55 63952.0 56 63952.0 57 63952.0 58 53335.5 59 42719.0 60 42719.0 61 42719.0 62 34227.5 63 25736.0 64 25736.0 65 25736.0 66 19459.0 67 13182.0 68 13182.0 69 13182.0 70 9540.5 71 5899.0 72 5899.0 73 5899.0 74 4099.5 75 2300.0 76 2300.0 77 2300.0 78 1558.5 79 817.0 80 817.0 81 817.0 82 545.0 83 273.0 84 273.0 85 273.0 86 175.5 87 78.0 88 78.0 89 78.0 90 48.0 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11164574404210165 2 0.11289086386413252 3 0.016048211039509035 4 0.0 5 0.006502292403939006 6 0.3419929111178132 7 0.023380583324801956 8 0.010652691810708584 9 0.011621118338954819 10 0.004150399406769578 11 0.005948905816369729 12 4.1503994067695785E-4 13 5.533865875692771E-4 14 0.0 15 0.003181972878523343 16 8.300798813539157E-4 17 1.3834664689231927E-4 18 5.533865875692771E-4 19 4.1503994067695785E-4 20 6.917332344615963E-4 21 0.012589544867201053 22 0.0 23 0.0 24 1.3834664689231927E-4 25 0.00567221252258509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 722822.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.12137780164113 #Duplication Level Percentage of deduplicated Percentage of total 1 92.18894395523782 78.47249927548118 2 6.193435323573966 10.54387496135938 3 0.8717061061727773 2.2260247438659144 4 0.26897802865092146 0.9158312158854295 5 0.12446045126830875 0.5297122546886227 6 0.08359808613855763 0.42695905642165816 7 0.044907868285809165 0.26758337358358897 8 0.04035511182105258 0.2748066175637832 9 0.025463817635966033 0.19507637171368414 >10 0.13230817802204362 2.123764439450707 >50 0.013978446810297482 0.8084059444774527 >100 0.009751747719354524 1.5875322078356842 >500 0.0016252912793909898 1.0883761088529347 >1k 4.8758738381729695E-4 0.5395534288199371 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1476 0.20419965081306327 No Hit GTCCTACAGTGGACATTTCTAAATT 1224 0.1693362957961988 No Hit CTGTAGGACGTGGAATATGGCAAGA 1200 0.16601597627078313 No Hit GTCCTACAGTGTGCATTTCTCATTT 968 0.13391955419176504 No Hit GGTATCAACGCAGAGTACTTTTTTT 928 0.12838568831607228 No Hit GTCCTAAAGTGTGTATTTCTCATTT 923 0.1276939550816107 No Hit CTTTAGGACGTGAAATATGGCGAGG 913 0.12631048861268748 No Hit GTACATGGGAAGCAGTGGTATCAAC 816 0.11289086386413252 No Hit CTGTAGGACCTGGAATATGGCGAGA 777 0.10749534463533206 No Hit TATCAACGCAGAGTACTTTTTTTTT 731 0.10113139887828537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 35 0.002187027 16.265198 13 TAGGACC 310 0.0 15.049267 4 GTCCTAT 120 4.9112714E-11 13.50397 1 CTAGGTA 50 0.0014735034 13.32935 4 TTAGGAC 310 0.0 12.592243 3 TAGGACT 130 1.891749E-10 12.450493 4 GCCTAAC 55 0.0029889368 12.131909 1 GATTAGG 55 0.0029889368 12.131909 1 TGCGCCA 55 0.0030947935 12.075677 12 GTGCGCC 55 0.0030947935 12.075677 11 ACCTATA 80 2.7639142E-5 11.915268 2 CTGGACT 80 2.7975326E-5 11.901206 4 TGTAGGA 765 0.0 11.837389 2 GTACAGT 115 6.8368536E-8 11.590739 6 GTAGGAC 775 0.0 11.54801 3 GTATTAA 75 2.0067615E-4 11.438658 1 GATTAAA 150 1.6552804E-10 11.438658 1 GTCCTAA 270 0.0 11.297439 1 TCCTATA 135 4.4638E-9 11.297439 2 TATAGGA 135 4.4638E-9 11.297439 2 >>END_MODULE