FastQCFastQC Report
Thu 26 May 2016
SRR1926947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708680
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12710.1793475193317153No Hit
GTATCAACGCAGAGTACTTTTTTTT10930.15423040018061748No Hit
GTCCTACAGTGTGCATTTCTCATTT10300.1453406332900604No Hit
CTGTAGGACGTGGAATATGGCAAGA10090.1423773776598747No Hit
GTCCTAAAGTGTGTATTTCTCATTT7800.11006378054975448No Hit
GTACATGGGAAGCAGTGGTATCAAC7470.10540723598803409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAC400.00514234414.3049363
TAGGACC2650.013.6519044
ATAGGAC751.418618E-512.7154993
CCAGTAC1001.3677709E-712.3976113
GTCATAC701.04043116E-412.2744421
TCTACAC1353.45608E-1012.0090823
GCGCCAC802.9169252E-511.85263813
GGTCTAT657.6902355E-411.7498931
AGGACCT4400.011.4628895
GTCCTAC6750.011.4561451
AGGCGTG752.0392697E-411.4188257
CGTGCGC752.1059663E-411.37933410
CTGTAGG7450.011.2767441
TCTGGAC1104.7124922E-711.27055553
TGTAGGA7700.011.1474952
GACCCTA600.005953243511.06246211
CGCCACC600.005953243511.06246214
TGGCGAG5200.010.94089618
GGACGTG4350.010.9375726
CATTTCT6950.010.914659514
Produced by FastQC (version 0.11.5)