FastQCFastQC Report
Thu 26 May 2016
SRR1926947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708680
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17640.24891347293559857No Hit
GTCCTACAGTGGACATTTCTAAATT12810.18075859344132753No Hit
CTGTAGGACGTGGAATATGGCAAGA11930.16834114127673985No Hit
GTCCTACAGTGTGCATTTCTCATTT11420.16114466331771743No Hit
GGTATCAACGCAGAGTACTTTTTTT11370.16043912626291132No Hit
GTCCTAAAGTGTGTATTTCTCATTT9140.12897217361855845No Hit
GTACATGGGAAGCAGTGGTATCAAC9120.128689958796636No Hit
TATCAACGCAGAGTACTTTTTTTTT8990.1268555624541401No Hit
CTGTAGGACCTGGAATATGGCGAGA8250.11641361404300954No Hit
CTTTAGGACGTGAAATATGGCGAGG7790.10992267313879324No Hit
CCCATGTACTCTGCGTTGATACCAC7380.10413726928938306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT400.00531884814.23237219
TTATAAC551.9176747E-413.851933
GTTTAGG655.2612384E-513.201871
TAGGACC4650.012.9024014
GTAGGAC7250.012.4786763
TGTAGGA7950.012.472162
ATAGCAC1001.3931094E-712.380163
CCAACGA550.003094161212.07595319
GTATAAA1052.586312E-711.8048461
CTAACCT657.8515813E-411.7208624
CTTATAC657.8515813E-411.7208623
TGGCGAG5800.011.61492418
TTAAGAC1156.8137524E-811.5934613
CTGTAGG8150.011.5820071
GGCGAGA3700.011.53976219
GTCCTAA3400.011.4977061
CTAGGAC1501.6734703E-1011.427843
GGTCTAC855.1194445E-511.2172751
CCTAATA600.00571617511.1230082
GGCGAGG2150.011.03284619