FastQCFastQC Report
Thu 26 May 2016
SRR1926946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638063
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12710.19919663105367338No Hit
CTGTAGGACGTGGAATATGGCAAGA10900.1708295262380047No Hit
GTATCAACGCAGAGTACTTTTTTTT10600.16612779615805962No Hit
GTCCTACAGTGTGCATTTCTCATTT9960.1560974386541768No Hit
GTCCTAAAGTGTGTATTTCTCATTT9200.1441863891183159No Hit
GTACATGGGAAGCAGTGGTATCAAC7750.12146136039858133No Hit
CTTTAGGACGTGAAATATGGCGAGG7160.11221462457468934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG702.5454938E-816.2591718
CCAACGA456.83865E-414.75369119
CGGTTTC400.00533197614.22677413
GCCGGTT400.00533197614.22677411
TAGGACC2800.013.9394054
GTCCTAC6450.013.8997671
GTGTAGG903.4420736E-813.7765421
GCAGCAC903.4779077E-813.765693
TGTAGGA7750.013.1587142
TCCTACA7050.012.978172
GTAGGAC7200.012.7067913
CTGTAGG7750.012.3065891
AGGACCT4900.012.2337025
TGGCGAG5850.011.99750718
GGCGAGG1900.011.98044119
GTAGAAC2400.011.9126173
CAGGACT802.8004415E-511.8994914
CCTACAG7500.011.6902483
GGACCTG5050.011.6809886
GGCGAGA4150.011.65566919
Produced by FastQC (version 0.11.5)