##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926946_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 638063 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.244649822979863 31.0 27.0 31.0 16.0 33.0 2 27.825409089698038 31.0 28.0 31.0 16.0 34.0 3 27.752065234937614 31.0 28.0 31.0 16.0 34.0 4 31.4846480676673 35.0 32.0 37.0 17.0 37.0 5 31.24419845689219 35.0 32.0 37.0 17.0 37.0 6 30.93577750159467 35.0 32.0 37.0 11.0 37.0 7 31.06328842136278 35.0 32.0 37.0 12.0 37.0 8 30.877919578474227 35.0 32.0 37.0 11.0 37.0 9 32.8476200625957 37.0 32.0 39.0 17.0 39.0 10 32.945259323922556 37.0 33.0 39.0 17.0 39.0 11 33.132239292985176 37.0 33.0 39.0 17.0 39.0 12 32.97322678168143 37.0 33.0 39.0 16.0 39.0 13 33.09481665603553 38.0 33.0 39.0 17.0 39.0 14 34.08279433222111 39.0 34.0 40.0 17.0 41.0 15 33.634470263908106 39.0 33.0 40.0 10.0 41.0 16 33.72909101452365 39.0 33.0 40.0 11.0 41.0 17 33.96159940319373 39.0 33.0 40.0 13.0 41.0 18 34.0138324898952 39.0 34.0 41.0 11.0 41.0 19 33.36364120784311 39.0 33.0 40.0 10.0 41.0 20 33.63097687845871 39.0 33.0 40.0 10.0 41.0 21 33.6387237623871 39.0 33.0 40.0 2.0 41.0 22 33.670540369838086 39.0 34.0 41.0 2.0 41.0 23 33.64959102784521 39.0 34.0 41.0 2.0 41.0 24 32.31492344799808 38.0 32.0 40.0 2.0 41.0 25 32.90883972272331 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.3386117003047282 1101 2 -0.3944430030228787 1101 3 -0.8133856307265965 1101 4 -0.418566198221594 1101 5 -0.5797784044195993 1101 6 -2.455323589473231 1101 7 -0.4473891008036155 1101 8 -0.023681962278121915 1101 9 -0.03526983860495392 1101 10 -9.291691105781123 1101 11 -3.965399351553671 1101 12 -1.710432941948195 1101 13 -0.882671745809759 1101 14 -0.5750690292916403 1101 15 -1.8786743439573783 1101 16 -0.452739421728225 1101 17 -0.3056398016786659 1101 18 -0.12428389967571718 1101 19 -0.6248675930217331 1101 20 -0.0722717885666313 1101 21 -0.11729990308991489 1101 22 -0.11040414073278981 1101 23 -0.2658671425407917 1101 24 -0.38983854938130946 1101 25 -0.3137620586178187 1102 1 -0.695529441066089 1102 2 -0.6579219992974465 1102 3 -0.7572408099326964 1102 4 -0.5312305240041475 1102 5 -0.3739804341374082 1102 6 -1.4058984620806037 1102 7 2.6323508470582624E-4 1102 8 0.5339102100337882 1102 9 0.004776408129941956 1102 10 0.11566233917241675 1102 11 -0.2765713637148508 1102 12 -0.10661758631936635 1102 13 -0.3127802971736102 1102 14 -0.590732969684737 1102 15 0.021110480262208853 1102 16 -0.07623854389667883 1102 17 -0.22779969548246015 1102 18 -0.5161682400174286 1102 19 -0.09896299832298183 1102 20 -0.17597687998957667 1102 21 -0.23320578242019252 1102 22 -0.30811107346091404 1102 23 -0.6448013265856716 1102 24 -3.900598408928264 1102 25 -2.403055725384405 1103 1 -0.29245693406340934 1103 2 -0.2872124955988191 1103 3 0.2711970071391008 1103 4 0.07591047484246971 1103 5 -0.2480362647896328 1103 6 0.4592891143418285 1103 7 0.37390172337310545 1103 8 0.638440922095743 1103 9 0.16298927224760007 1103 10 0.10571441482737498 1103 11 -0.11560078797750606 1103 12 -0.316910441418095 1103 13 -0.07429786240909664 1103 14 0.024774388548941317 1103 15 0.2808567794652461 1103 16 0.13507652899189537 1103 17 -0.3795085227612631 1103 18 -0.1805523510890339 1103 19 -1.8570843723224435 1103 20 -0.7673965377905176 1103 21 -0.3179425423692024 1103 22 -0.1581258159444161 1103 23 -0.38629404640043674 1103 24 1.1606988862596168 1103 25 0.5359179809626653 1104 1 -0.8271463090465936 1104 2 -0.38469144197598126 1104 3 -1.795784993022334 1104 4 -0.8300501891874497 1104 5 -1.0197358908019538 1104 6 -2.2603029969396857 1104 7 -0.6797738490432863 1104 8 -6.076963022998427E-4 1104 9 -0.27675781522246723 1104 10 -0.069748764875051 1104 11 -0.21741476274006288 1104 12 -0.3757551154568972 1104 13 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8 1.850556962332238 1110 9 1.591138384962889 1110 10 1.67278753416727 1110 11 1.2522546842787747 1110 12 1.2090085546079763 1110 13 1.2181658012697483 1110 14 0.9911814649131614 1110 15 1.6707711062371828 1110 16 1.29961611440595 1110 17 0.8298441028513679 1110 18 1.1034050946435272 1110 19 -1.1076838320711957 1110 20 0.8360339733411024 1110 21 0.7849411856172495 1110 22 0.8923436379466949 1110 23 1.1253453759268623 1110 24 -0.09160624191309807 1110 25 0.17635696670627254 1111 1 0.8144271521114383 1111 2 0.4511907766955545 1111 3 1.0880375741236747 1111 4 0.9623381544175125 1111 5 0.9133874448139636 1111 6 1.2422702359599604 1111 7 1.0218041772825153 1111 8 1.311123263143795 1111 9 0.8368631236803736 1111 10 0.7718216528378008 1111 11 0.6713077511456547 1111 12 0.5687263575705686 1111 13 0.5819264819747687 1111 14 0.6189685777562275 1111 15 -0.3868778873633332 1111 16 -0.20584489878628176 1111 17 0.3894028124672815 1111 18 0.25955193720665903 1111 19 0.9957983458988409 1111 20 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-3.6272059582708494 2216 16 -2.658560341417342 2216 17 -3.0033598065361815 2216 18 -2.581412299934783 2216 19 -2.165732432723807 2216 20 -3.314721391869739 2216 21 -5.557279258659861 2216 22 -4.401562455175792 2216 23 -4.245258131854264 2216 24 -4.239941771531569 2216 25 -3.7710341603017596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39744.0 3 4.0 4 322.0 5 1841.0 6 2266.0 7 1812.0 8 1619.0 9 1468.0 10 1380.0 11 1272.0 12 1260.0 13 1248.0 14 1217.0 15 1321.0 16 1396.0 17 1489.0 18 1719.0 19 2101.0 20 2825.0 21 3549.0 22 4466.0 23 5267.0 24 6158.0 25 7263.0 26 7988.0 27 8761.0 28 9777.0 29 11581.0 30 13802.0 31 16901.0 32 21267.0 33 27709.0 34 37671.0 35 51644.0 36 75143.0 37 120407.0 38 142405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90041740080842 18.781151950854117 13.045267101969541 25.273163546367922 2 13.933016576388846 19.95600133063023 42.996146193581595 23.11483589939933 3 19.09404461517217 24.654621574461142 29.577637178473243 26.673696631893446 4 11.469046700478955 16.762802718784013 39.15324996314523 32.6149006175918 5 12.761294752237317 38.290938333262794 36.15872612782927 12.789040786670617 6 31.5787988613135 37.55705973087872 18.538298992989805 12.325842414817973 7 27.569157847423693 31.731672895404355 22.533781341446932 18.165387915725024 8 24.224009504233532 38.12049381784809 21.425697888576693 16.229798789341682 9 25.832654084895605 16.0447363471064 20.661483478588 37.461126089409994 10 14.063315727340711 30.138856880368614 34.96035908072575 20.837468311564926 11 33.72744614584003 23.27120426726523 24.783023732979295 18.21832585391545 12 22.773528120885324 25.495799109662048 32.207661922377575 19.52301084707505 13 28.51721694859995 22.668250843291908 26.063989867957417 22.750542340150727 14 19.831088706643595 23.215584480726307 28.13119153311765 28.82213527951245 15 22.65611039879118 31.98386151246595 23.381997498346337 21.978030590396532 16 20.910572928912924 29.22439062622463 29.0408339211537 20.824202523708752 17 20.676158424470614 29.64133946331806 28.549259589596165 21.133242522615163 18 20.53616352940623 28.72564371442858 30.565051718165385 20.1731410379998 19 23.118898175958265 27.649688677648122 27.78606855989115 21.44534458650247 20 22.652496610315612 28.371305635889115 28.82822725384621 20.147970499949057 21 22.108007028466545 27.69629626146397 27.778901120259818 22.416795589809663 22 21.834358203480743 29.144454180231893 28.15850976617913 20.862677850108234 23 21.321119382280052 28.661559755407477 28.440699167187862 21.576621695124608 24 22.633074647827716 27.778622472251786 28.88463232539512 20.703670554525377 25 21.718283190224053 28.161178982215578 28.80728681733111 21.313251010229255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 196.0 1 196.0 2 283.0 3 370.0 4 370.0 5 370.0 6 546.0 7 722.0 8 722.0 9 722.0 10 1084.5 11 1447.0 12 1447.0 13 1447.0 14 2456.5 15 3466.0 16 3466.0 17 3466.0 18 5942.5 19 8419.0 20 8419.0 21 8419.0 22 13192.0 23 17965.0 24 17965.0 25 17965.0 26 25520.0 27 33075.0 28 33075.0 29 33075.0 30 43094.0 31 53113.0 32 53113.0 33 53113.0 34 60156.0 35 67199.0 36 67199.0 37 67199.0 38 72183.0 39 77167.0 40 77167.0 41 77167.0 42 79838.0 43 82509.0 44 82509.0 45 82509.0 46 83655.5 47 84802.0 48 84802.0 49 84802.0 50 79714.0 51 74626.0 52 74626.0 53 74626.0 54 64214.0 55 53802.0 56 53802.0 57 53802.0 58 45354.0 59 36906.0 60 36906.0 61 36906.0 62 29502.0 63 22098.0 64 22098.0 65 22098.0 66 16780.0 67 11462.0 68 11462.0 69 11462.0 70 8397.5 71 5333.0 72 5333.0 73 5333.0 74 3757.5 75 2182.0 76 2182.0 77 2182.0 78 1497.0 79 812.0 80 812.0 81 812.0 82 532.5 83 253.0 84 253.0 85 253.0 86 174.0 87 95.0 88 95.0 89 95.0 90 64.5 91 34.0 92 34.0 93 34.0 94 20.0 95 6.0 96 6.0 97 6.0 98 5.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1982562850376844 2 0.12083446305458867 3 0.12396894977455203 4 0.06660784279922202 5 0.021001061023754708 6 0.02115778535975288 7 0.2766184530367691 8 0.26878223623686065 9 0.016142606607811454 10 0.033225559231611926 11 0.014105190239835252 12 0.016142606607811454 13 0.013635017231840744 14 0.014888811919826097 15 0.013321568559844404 16 0.017709849967793147 17 0.016612779615805963 18 0.013478292895842573 19 0.021784682703745555 20 0.015358984927820606 21 0.013478292895842573 22 0.015045536255824268 23 0.016299330943809625 24 0.014732087583827928 25 0.013948465903837083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 638063.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.66846978619918 #Duplication Level Percentage of deduplicated Percentage of total 1 94.12258768932296 81.5746064735097 2 4.455396044882743 7.722847150029427 3 0.7018478091796182 1.824842269331815 4 0.2544647544951776 0.8821628354647155 5 0.12645028985294593 0.5479626562788088 6 0.07580620401082576 0.3942004621151222 7 0.04310191076913066 0.2614903655855298 8 0.03349999007938007 0.23227143024261793 9 0.022989133550270706 0.17931897238612815 >10 0.1364914729138653 2.2296073910165535 >50 0.014316739571201888 0.8473607083771496 >100 0.011054522829891883 1.874692770945914 >500 0.0014497734851129068 0.8913272010791978 >1k 5.436650569173401E-4 0.5373093136373455 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1271 0.19919663105367338 No Hit CTGTAGGACGTGGAATATGGCAAGA 1090 0.1708295262380047 No Hit GTATCAACGCAGAGTACTTTTTTTT 1060 0.16612779615805962 No Hit GTCCTACAGTGTGCATTTCTCATTT 996 0.1560974386541768 No Hit GTCCTAAAGTGTGTATTTCTCATTT 920 0.1441863891183159 No Hit GTACATGGGAAGCAGTGGTATCAAC 775 0.12146136039858133 No Hit CTTTAGGACGTGAAATATGGCGAGG 716 0.11221462457468934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5672433599816946E-4 2 0.0 0.0 0.0 0.0 3.134486719963389E-4 3 0.0 0.0 0.0 0.0 3.134486719963389E-4 4 0.0 0.0 0.0 0.0 3.134486719963389E-4 5 0.0 0.0 0.0 0.0 3.134486719963389E-4 6 0.0 0.0 0.0 0.0 3.134486719963389E-4 7 0.0 0.0 0.0 0.0 3.134486719963389E-4 8 0.0 0.0 0.0 0.0 3.134486719963389E-4 9 0.0 0.0 0.0 0.0 3.134486719963389E-4 10 0.0 0.0 0.0 0.0 3.134486719963389E-4 11 0.0 0.0 0.0 0.0 3.134486719963389E-4 12 0.0 0.0 0.0 0.0 3.134486719963389E-4 13 0.0 0.0 0.0 0.0 4.701730079945084E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 70 2.5454938E-8 16.25917 18 CCAACGA 45 6.83865E-4 14.753691 19 CGGTTTC 40 0.005331976 14.226774 13 GCCGGTT 40 0.005331976 14.226774 11 TAGGACC 280 0.0 13.939405 4 GTCCTAC 645 0.0 13.899767 1 GTGTAGG 90 3.4420736E-8 13.776542 1 GCAGCAC 90 3.4779077E-8 13.76569 3 TGTAGGA 775 0.0 13.158714 2 TCCTACA 705 0.0 12.97817 2 GTAGGAC 720 0.0 12.706791 3 CTGTAGG 775 0.0 12.306589 1 AGGACCT 490 0.0 12.233702 5 TGGCGAG 585 0.0 11.997507 18 GGCGAGG 190 0.0 11.980441 19 GTAGAAC 240 0.0 11.912617 3 CAGGACT 80 2.8004415E-5 11.899491 4 CCTACAG 750 0.0 11.690248 3 GGACCTG 505 0.0 11.680988 6 GGCGAGA 415 0.0 11.655669 19 >>END_MODULE