FastQCFastQC Report
Thu 26 May 2016
SRR1926946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638063
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17300.27113310127683315No Hit
CTGTAGGACGTGGAATATGGCAAGA13900.21784682703745556No Hit
GTCCTACAGTGGACATTTCTAAATT13170.20640595050958918No Hit
GGTATCAACGCAGAGTACTTTTTTT10780.16894883420602666No Hit
GTCCTACAGTGTGCATTTCTCATTT10250.1606424443981237No Hit
GTCCTAAAGTGTGTATTTCTCATTT9320.14606708115029393No Hit
GTACATGGGAAGCAGTGGTATCAAC9130.14308931876632872No Hit
TATCAACGCAGAGTACTTTTTTTTT8950.14026828071836167No Hit
CTTTAGGACGTGAAATATGGCGAGG8660.13572327497441475No Hit
CTGTAGGACCTGGAATATGGCGAGA7830.12271515508656668No Hit
CCCATGTACTCTGCGTTGATACCAC7230.11331169492667652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA651.9368781E-716.05576519
TAGGACC2900.015.1107274
CGTTTCC653.4136647E-614.59500614
TCCAACG801.3051977E-714.23124618
CTAGGAC1204.9112714E-1113.4956313
CCGTTTC751.4966663E-512.64900513
AGGACCT4700.012.5666465
ATCCCGT853.994859E-612.27697510
GACCGTG550.003097233812.0740497
CCCGTTT802.9012876E-511.85844212
TCCCGTT802.903616E-511.85751211
TTTAGAA1950.011.7339482
TTAGGCT657.828086E-411.7247124
GTCCTAC6500.011.4405991
GTAGGAC7250.011.4315943
TAGGACA2100.011.3408674
GTATTGG600.005715892711.1228041
TGGACTG1201.2221062E-711.1140495
TTAGGAC3350.011.0903523
GGACTGT951.3167453E-511.0296166
Produced by FastQC (version 0.11.5)