##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926946_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 638063 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.82111484289169 31.0 30.0 33.0 25.0 34.0 2 30.1334680117794 31.0 30.0 34.0 26.0 34.0 3 30.208647108514363 31.0 30.0 34.0 26.0 34.0 4 33.80182050988695 35.0 35.0 37.0 28.0 37.0 5 33.790542626668525 37.0 35.0 37.0 30.0 37.0 6 33.74868469101013 37.0 35.0 37.0 29.0 37.0 7 33.73345108555111 37.0 35.0 37.0 29.0 37.0 8 33.75361994035072 37.0 35.0 37.0 29.0 37.0 9 35.27778761658332 39.0 35.0 39.0 29.0 39.0 10 35.15841068985351 39.0 35.0 39.0 29.0 39.0 11 35.19814501075913 39.0 35.0 39.0 29.0 39.0 12 35.09862819188701 39.0 35.0 39.0 29.0 39.0 13 35.11299667901132 39.0 35.0 39.0 29.0 39.0 14 36.201917365526604 40.0 36.0 41.0 29.0 41.0 15 36.18555221036167 40.0 36.0 41.0 29.0 41.0 16 36.1727055165399 40.0 36.0 41.0 29.0 41.0 17 36.14772209013844 40.0 36.0 41.0 29.0 41.0 18 36.118272020161015 40.0 36.0 41.0 29.0 41.0 19 36.18535630494168 40.0 37.0 41.0 29.0 41.0 20 36.14668927676421 40.0 37.0 41.0 28.0 41.0 21 36.08905860393096 40.0 36.0 41.0 28.0 41.0 22 35.99998276032304 40.0 36.0 41.0 27.0 41.0 23 35.9299802684061 40.0 36.0 41.0 27.0 41.0 24 35.86591919606685 40.0 36.0 41.0 27.0 41.0 25 35.7428858905782 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4530615936183082 1101 2 0.43509787407307954 1101 3 0.7795187828903494 1101 4 0.6464455589631797 1101 5 0.46505769848153733 1101 6 0.49234631249164806 1101 7 0.45856313431200846 1101 8 0.4659379442024729 1101 9 0.6025156630053701 1101 10 0.5670710730620883 1101 11 0.5850539424699903 1101 12 0.7400463836555744 1101 13 0.6354421960640622 1101 14 0.8031008532933726 1101 15 0.7255489732289462 1101 16 0.8063380600116474 1101 17 0.9027081180821952 1101 18 0.752953251409707 1101 19 0.6731829273983081 1101 20 0.9511264624192322 1101 21 0.8301178861690772 1101 22 0.6123773658094862 1101 23 0.5888032809911365 1101 24 0.6904723513300652 1101 25 0.8997738349849698 1102 1 -0.7335793090494427 1102 2 -0.6481483897902365 1102 3 -0.49407454558992825 1102 4 -0.6058528609400398 1102 5 -0.8103747941318318 1102 6 -0.5753548393946204 1102 7 -0.6420898867131299 1102 8 -0.7928789101631324 1102 9 -0.6252473652993444 1102 10 -0.6746762399169697 1102 11 -0.6434852173809915 1102 12 -0.6827943648563917 1102 13 -0.8037797454423838 1102 14 -0.7701499067802828 1102 15 -0.5096413663419881 1102 16 -0.6469944882690868 1102 17 -0.6499714091734035 1102 18 -0.7082487354870537 1102 19 -0.7332702049615634 1102 20 -0.5366759612522429 1102 21 -0.5413622234528148 1102 22 -0.5557787597418411 1102 23 -0.42409227846589204 1102 24 -0.5591476118438266 1102 25 -0.45442262799623023 1103 1 -0.4056230075049534 1103 2 -0.2655007264738707 1103 3 -0.040677053546534836 1103 4 -0.4334080802128071 1103 5 -0.26267093593718016 1103 6 -0.25672099065250364 1103 7 -0.20279206253024995 1103 8 -0.28942526660169676 1103 9 -0.2632782031060117 1103 10 -0.13697342236821441 1103 11 -0.19049314363388703 1103 12 -0.3300067346672577 1103 13 -0.2263968831458527 1103 14 -0.20778378153854504 1103 15 -0.27900822003584835 1103 16 -0.1964912926189868 1103 17 -0.11830433781111083 1103 18 -0.2955524219751311 1103 19 -0.07520476567891166 1103 20 -0.07114952451050982 1103 21 -0.3017068940450258 1103 22 -0.17450561621075167 1103 23 -0.2947941941215433 1103 24 -0.2701483229518544 1103 25 -0.46281933955521026 1104 1 -0.36563376974026696 1104 2 -0.2708248042848318 1104 3 -0.14505674520849254 1104 4 -0.167998442631081 1104 5 -0.3473137642526183 1104 6 -0.3228151064003413 1104 7 -0.20547831646519654 1104 8 -0.3492234746895164 1104 9 -0.3679958289569001 1104 10 -0.3792926840788695 1104 11 -0.32512009765223837 1104 12 -0.31400436697548173 1104 13 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22 0.2776675212498958 1108 23 0.08950276285641934 1108 24 0.25943090055286433 1108 25 0.29504844638393024 1109 1 0.7032095579407347 1109 2 0.47376899870576494 1109 3 0.6861102040746871 1109 4 0.6459710347852905 1109 5 0.5113450919212639 1109 6 0.5105007560938759 1109 7 0.5239405212101573 1109 8 0.4620482996283499 1109 9 0.4499898860628875 1109 10 0.5919955333816915 1109 11 0.5965920445809516 1109 12 0.6825230468264749 1109 13 0.6125612009131416 1109 14 0.6949103790806959 1109 15 0.6400712820953842 1109 16 0.6599603304592776 1109 17 0.6883477912108376 1109 18 0.6468623130563174 1109 19 0.4708653388533435 1109 20 0.5431903830065181 1109 21 0.4508391213856271 1109 22 0.40243104830400966 1109 23 0.47726829391854864 1109 24 0.4911202793103797 1109 25 0.48345726401920075 1110 1 0.4069704934664031 1110 2 0.09137167393573975 1110 3 0.4508746727711177 1110 4 0.2502035962938507 1110 5 0.35321759194138735 1110 6 0.4657143962255432 1110 7 0.4922325269083032 1110 8 0.443322092193398 1110 9 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15517.0 3 14.0 4 222.0 5 1634.0 6 2244.0 7 1674.0 8 1327.0 9 1194.0 10 988.0 11 909.0 12 844.0 13 826.0 14 784.0 15 804.0 16 881.0 17 816.0 18 1044.0 19 1192.0 20 1492.0 21 1916.0 22 2503.0 23 2999.0 24 3563.0 25 4286.0 26 5151.0 27 5608.0 28 6576.0 29 8035.0 30 10354.0 31 13152.0 32 17147.0 33 22928.0 34 30920.0 35 43145.0 36 63787.0 37 109697.0 38 251890.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.51607103072371 19.75545925523803 10.757690340436616 27.97077937360165 2 13.708679193186294 20.967774830135813 40.817293437147505 24.506252539530387 3 18.50655355860897 24.97108032615749 29.250565070049877 27.271801045183658 4 11.768430390102544 17.671609229809597 38.29982305822466 32.260137321863205 5 12.512009529257801 39.006324104477024 35.55698355105911 12.924682815206062 6 31.138371445734254 38.452901752719654 17.977563427191214 12.431163374354878 7 27.872316412520064 32.05194121187801 21.57105863764045 18.50468373796148 8 24.58452650206032 38.91226898764434 19.844923331562192 16.658281178733148 9 25.98019777150479 16.201809065389362 20.147207954746722 37.67078520835913 10 14.311194422850265 30.399729167189104 34.228533992025476 21.060542417935153 11 33.71873780427597 23.4864763624338 24.3712296111004 18.42355622218983 12 23.06554242547723 25.706046453311597 31.8073535404194 19.421057580791775 13 28.819637086283695 22.668785596293752 25.70424632243464 22.807330994987918 14 20.085822380897152 23.4293846052578 27.848077459557224 28.63671555428783 15 22.34668129456939 32.13509913016222 23.383747355222944 22.13447222004545 16 21.121021344354222 29.35691101634483 28.650082045447068 20.87198559385388 17 20.776350887531304 29.82954634502603 28.31981845024465 21.074284317198018 18 20.68799603796532 28.682905575686146 30.284802587860625 20.344295798487906 19 22.682086251671073 27.811987217563157 28.00350435615292 21.50242217461285 20 22.607925649042794 28.283768640634428 28.858640712791217 20.24966499753156 21 22.014978009473325 27.520916862329585 27.755555625217475 22.708549502979615 22 22.07963790409411 29.15746564210744 27.77797803665155 20.984918417146897 23 21.300414695842697 28.718862548545744 28.421867604512443 21.558855151099117 24 22.213079940632635 27.75863122804873 29.21288090010203 20.81540793121661 25 21.73987320444803 28.22713487504604 28.739645944188013 21.293345976317912 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 256.0 3 280.0 4 280.0 5 280.0 6 448.0 7 616.0 8 616.0 9 616.0 10 994.5 11 1373.0 12 1373.0 13 1373.0 14 2214.0 15 3055.0 16 3055.0 17 3055.0 18 5452.5 19 7850.0 20 7850.0 21 7850.0 22 12584.0 23 17318.0 24 17318.0 25 17318.0 26 24600.0 27 31882.0 28 31882.0 29 31882.0 30 42194.0 31 52506.0 32 52506.0 33 52506.0 34 59552.5 35 66599.0 36 66599.0 37 66599.0 38 71312.0 39 76025.0 40 76025.0 41 76025.0 42 79340.5 43 82656.0 44 82656.0 45 82656.0 46 84222.0 47 85788.0 48 85788.0 49 85788.0 50 80878.0 51 75968.0 52 75968.0 53 75968.0 54 65528.5 55 55089.0 56 55089.0 57 55089.0 58 46169.0 59 37249.0 60 37249.0 61 37249.0 62 29915.5 63 22582.0 64 22582.0 65 22582.0 66 17339.0 67 12096.0 68 12096.0 69 12096.0 70 8807.0 71 5518.0 72 5518.0 73 5518.0 74 3843.5 75 2169.0 76 2169.0 77 2169.0 78 1510.0 79 851.0 80 851.0 81 851.0 82 547.5 83 244.0 84 244.0 85 244.0 86 168.5 87 93.0 88 93.0 89 93.0 90 57.0 91 21.0 92 21.0 93 21.0 94 12.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11049065687870949 2 0.1006170237108248 3 0.013948465903837083 4 0.0 5 0.004388281407948745 6 0.33491990602808813 7 0.01739640129579681 8 0.00877656281589749 9 0.008463114143901152 10 0.004858454415943254 11 0.005015178751941423 12 4.701730079945084E-4 13 7.836216799908472E-4 14 1.5672433599816946E-4 15 0.002037416367976203 16 3.134486719963389E-4 17 1.5672433599816946E-4 18 0.0010970703519871863 19 0.0 20 0.0012537946879853557 21 0.009560184495888337 22 0.0 23 7.836216799908472E-4 24 3.134486719963389E-4 25 0.004388281407948745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 638063.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.79910768970134 #Duplication Level Percentage of deduplicated Percentage of total 1 92.46359280170824 77.48366570567045 2 5.814848010070382 9.745581491902671 3 0.8612452249827821 2.1651474406671967 4 0.30670477149579534 1.0280634470208558 5 0.1387439156966107 0.5813308166375564 6 0.09469223499059996 0.47610748784134754 7 0.05870447011222761 0.34435675489609907 8 0.04348212065345308 0.2915010328972241 9 0.028406821342010304 0.21424196526851053 >10 0.15847837326199196 2.518112120865964 >50 0.015363355489654293 0.891311685378476 >100 0.013489628740226604 2.339496014420309 >500 0.0013114916827107414 0.8942869133780689 >1k 9.367797733648152E-4 1.0267971231552968 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1730 0.27113310127683315 No Hit CTGTAGGACGTGGAATATGGCAAGA 1390 0.21784682703745556 No Hit GTCCTACAGTGGACATTTCTAAATT 1317 0.20640595050958918 No Hit GGTATCAACGCAGAGTACTTTTTTT 1078 0.16894883420602666 No Hit GTCCTACAGTGTGCATTTCTCATTT 1025 0.1606424443981237 No Hit GTCCTAAAGTGTGTATTTCTCATTT 932 0.14606708115029393 No Hit GTACATGGGAAGCAGTGGTATCAAC 913 0.14308931876632872 No Hit TATCAACGCAGAGTACTTTTTTTTT 895 0.14026828071836167 No Hit CTTTAGGACGTGAAATATGGCGAGG 866 0.13572327497441475 No Hit CTGTAGGACCTGGAATATGGCGAGA 783 0.12271515508656668 No Hit CCCATGTACTCTGCGTTGATACCAC 723 0.11331169492667652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.5672433599816946E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 65 1.9368781E-7 16.055765 19 TAGGACC 290 0.0 15.110727 4 CGTTTCC 65 3.4136647E-6 14.595006 14 TCCAACG 80 1.3051977E-7 14.231246 18 CTAGGAC 120 4.9112714E-11 13.495631 3 CCGTTTC 75 1.4966663E-5 12.649005 13 AGGACCT 470 0.0 12.566646 5 ATCCCGT 85 3.994859E-6 12.276975 10 GACCGTG 55 0.0030972338 12.074049 7 CCCGTTT 80 2.9012876E-5 11.858442 12 TCCCGTT 80 2.903616E-5 11.857512 11 TTTAGAA 195 0.0 11.733948 2 TTAGGCT 65 7.828086E-4 11.724712 4 GTCCTAC 650 0.0 11.440599 1 GTAGGAC 725 0.0 11.431594 3 TAGGACA 210 0.0 11.340867 4 GTATTGG 60 0.0057158927 11.122804 1 TGGACTG 120 1.2221062E-7 11.114049 5 TTAGGAC 335 0.0 11.090352 3 GGACTGT 95 1.3167453E-5 11.029616 6 >>END_MODULE