FastQCFastQC Report
Thu 26 May 2016
SRR1926945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674774
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12950.19191610820808153No Hit
GTCCTACAGTGTGCATTTCTCATTT10530.15605224860471803No Hit
CTGTAGGACGTGGAATATGGCAAGA9990.14804956918909146No Hit
GTCCTAAAGTGTGTATTTCTCATTT8680.12863566171784924No Hit
GTATCAACGCAGAGTACTTTTTTTT8460.1253753108448162No Hit
CTGTAGGACCTGGAATATGGCGAGA7010.1038866346361893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGATC500.001488326413.3114478
CTTAGAC603.983911E-412.7049533
AGGACCG604.026685E-412.6888865
TCCAACG751.4999578E-512.64654818
GTCCTAA2950.012.60761451
TCTGGAC1251.3387762E-912.1967553
GTATTAA959.898704E-712.0461421
TAGGACT1209.669748E-911.899374
GTCTAGA751.9979585E-411.4438341
TAAAGTG3600.011.3671265
CGTGGAA2850.011.3153329
TAGGACC3200.011.3044024
TGTAGGA6750.011.1529572
CTGTAGG6850.011.1375521
GTAGGAC6550.011.0562193
CTTACAC951.3122542E-511.0332493
TTAGCCA700.001464234810.8794254
CTAAAGT3650.010.6931334
GTGTAGG909.1332564E-510.5961441
ACTACTC909.413304E-510.564648
Produced by FastQC (version 0.11.5)