##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926945_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 674774 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.159622332810688 31.0 27.0 31.0 16.0 33.0 2 27.73922379937579 31.0 28.0 31.0 16.0 34.0 3 27.65982536375142 31.0 28.0 31.0 15.0 34.0 4 31.390635975897116 35.0 32.0 37.0 17.0 37.0 5 31.174745914928554 35.0 32.0 37.0 16.0 37.0 6 30.8330315038813 35.0 32.0 37.0 11.0 37.0 7 30.93873652511804 35.0 32.0 37.0 11.0 37.0 8 30.76997928195218 35.0 32.0 37.0 10.0 37.0 9 32.74618168453438 37.0 32.0 39.0 17.0 39.0 10 32.844238219018514 37.0 33.0 39.0 15.0 39.0 11 33.02666818816374 37.0 33.0 39.0 17.0 39.0 12 32.868724936052665 37.0 33.0 39.0 11.0 39.0 13 32.977639920921675 38.0 33.0 39.0 13.0 39.0 14 33.977617691256626 39.0 33.0 40.0 11.0 41.0 15 33.53905455752593 39.0 33.0 40.0 10.0 41.0 16 33.624696565072156 39.0 33.0 40.0 10.0 41.0 17 33.84995124293467 39.0 33.0 41.0 10.0 41.0 18 33.901941983538194 39.0 34.0 41.0 10.0 41.0 19 33.230887674984515 39.0 32.0 40.0 8.0 41.0 20 33.5075269645837 39.0 33.0 41.0 2.0 41.0 21 33.52055799423214 39.0 33.0 41.0 2.0 41.0 22 33.537028694051635 39.0 33.0 41.0 2.0 41.0 23 33.53317851606612 39.0 34.0 41.0 2.0 41.0 24 32.18015365144478 38.0 31.0 40.0 2.0 41.0 25 32.78525847172535 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.25261731768361884 1101 2 -0.2692372649585941 1101 3 -1.0685661493064451 1101 4 -0.3817287861873666 1101 5 -0.18887859374147808 1101 6 -2.657728897694682 1101 7 -0.5333202052521138 1101 8 0.039825083695504304 1101 9 -0.0953630761276898 1101 10 -8.958912916502783 1101 11 -3.815398987160112 1101 12 -1.4651129095123636 1101 13 -0.6846274927296321 1101 14 -0.38709545517896515 1101 15 -1.7887095700974562 1101 16 -0.11600739813233929 1101 17 -0.27750175435653546 1101 18 -0.1297426642891395 1101 19 -0.8970315958523969 1101 20 -0.11779128153929719 1101 21 0.18076028631433871 1101 22 0.3690757185005964 1101 23 0.2070672327575025 1101 24 0.19650006006936138 1101 25 0.06561856519409304 1102 1 -0.5040017472832865 1102 2 -0.12016740613303512 1102 3 -0.3779739450491668 1102 4 -0.272313323129449 1102 5 -0.33565975898170564 1102 6 -1.1189466590303283 1102 7 -0.12620301992351912 1102 8 0.5799117243571921 1102 9 0.27730677886420807 1102 10 0.25790821461789193 1102 11 -0.0961549844382148 1102 12 0.039075361412216125 1102 13 -0.09502149688914585 1102 14 -0.2841588734597664 1102 15 0.4871690563030242 1102 16 0.22797328023337116 1102 17 -0.048286408225173716 1102 18 -0.16651798320152977 1102 19 0.09980371980138614 1102 20 0.053680555453581746 1102 21 -0.0955534602233925 1102 22 -0.06701912618834882 1102 23 -0.46434997461462046 1102 24 -3.8967507857313457 1102 25 -2.050954336597183 1103 1 -0.37071942866451124 1103 2 -0.4475575252205104 1103 3 0.1656627664147976 1103 4 -0.029054439395171272 1103 5 -0.5898852121358473 1103 6 0.3714737341018157 1103 7 0.18007657259644816 1103 8 0.37419648211701073 1103 9 0.04214488685597928 1103 10 0.24616685922069337 1103 11 -0.16563702566448057 1103 12 -0.2916195546526339 1103 13 -0.2675548557298413 1103 14 -0.2652148780510899 1103 15 0.21165611929025374 1103 16 0.10651362171311973 1103 17 -0.1497020665541271 1103 18 -0.2239621460022363 1103 19 -1.7558852179766014 1103 20 -0.5373663621417109 1103 21 -0.38818821080926824 1103 22 -0.2928979343570788 1103 23 -0.47726307423678804 1103 24 1.210461419539115 1103 25 0.4086994010277678 1104 1 -0.8379823298075593 1104 2 -0.7230800274922586 1104 3 -1.7682652071850882 1104 4 -0.6626045852653739 1104 5 -0.6550772347098608 1104 6 -2.247101998836154 1104 7 -0.806785544195364 1104 8 0.010979685522240601 1104 9 -0.16929516288336544 1104 10 -0.1197616882947301 1104 11 -0.2825627514285074 1104 12 -0.26092463858778103 1104 13 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2215 7 0.8868047523480413 2215 8 -0.4756657386959091 2215 9 0.26699069475115067 2215 10 0.16531079715976915 2215 11 0.1717973784907798 2215 12 -0.06320086670351088 2215 13 0.44301774295981033 2215 14 0.336291090891649 2215 15 0.26723137530944285 2215 16 0.5781427210347161 2215 17 0.06452067572063669 2215 18 -0.7910979020459834 2215 19 0.4373843692217676 2215 20 -3.1260340798305926 2215 21 -1.5959601181466212 2215 22 -0.7720363343320713 2215 23 -0.4128663773644021 2215 24 -0.2697872529126428 2215 25 -0.21342297904628538 2216 1 -0.04882900430231274 2216 2 -0.9260613832788387 2216 3 -0.20968247289138375 2216 4 -0.37196583241336967 2216 5 -0.1849881663477433 2216 6 -2.0802644507459185 2216 7 -10.24895888860258 2216 8 -10.80503829998625 2216 9 -5.970347181479845 2216 10 -3.30865535040774 2216 11 -2.437801364902363 2216 12 -1.799241217904445 2216 13 -1.7852592989149052 2216 14 -1.837029013124301 2216 15 -2.9964358734828487 2216 16 -1.8537481353139746 2216 17 -2.1502319743772915 2216 18 -1.909748119429345 2216 19 -1.2613116872114318 2216 20 -2.0093718259079303 2216 21 -5.0886256027327725 2216 22 -3.030930687891818 2216 23 -3.1766009316460533 2216 24 -3.53571079565965 2216 25 -2.7795593141152963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44528.0 3 8.0 4 380.0 5 2087.0 6 2352.0 7 2082.0 8 1655.0 9 1506.0 10 1406.0 11 1378.0 12 1314.0 13 1325.0 14 1357.0 15 1401.0 16 1523.0 17 1568.0 18 1891.0 19 2268.0 20 2874.0 21 3732.0 22 4755.0 23 5671.0 24 6503.0 25 7484.0 26 8380.0 27 9137.0 28 10241.0 29 12067.0 30 14361.0 31 17445.0 32 22129.0 33 28789.0 34 39110.0 35 53300.0 36 77995.0 37 125509.0 38 155263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82527146354972 18.051193875384385 12.468688704473339 25.65484595659256 2 14.662300608171968 19.354824351211523 42.12796389247864 23.854911148137873 3 19.648206535850736 23.51407693163693 29.050632441856152 27.787084090656183 4 11.682009435543316 16.02307125303481 38.3555873929751 33.93933191844677 5 13.133392924113659 37.756688768620265 36.036412635024256 13.073505672241815 6 32.84880790736008 36.65796042762358 18.00131038285711 12.491921282159227 7 28.514092030985083 31.16399120910473 21.778566323263032 18.543350436647156 8 24.863930769996568 37.62212055518326 20.757262597640725 16.75668607717945 9 25.994583689702043 15.68877116163106 19.972607166309185 38.34403798235771 10 14.297892402210783 29.56418862822307 34.68492777000261 21.452991199563538 11 35.01340689213566 22.597246288091803 23.91836988792757 18.470976931844962 12 23.22866099948862 24.85751765743465 31.615293969420954 20.298527373655777 13 29.113905288168656 22.041867200666413 25.519930868574697 23.324296642590234 14 20.25788339366127 22.35868885311704 27.51680140399558 29.86662634922611 15 23.10589497805558 31.47679049712521 22.729112040304805 22.6882024845144 16 21.619830839605942 28.63319824613934 28.12639892325526 21.62057199099946 17 21.265447150253692 28.999805822076695 27.939386842444623 21.795360185224986 18 21.17511042599235 28.14232354074645 29.660421545667447 21.02214448759375 19 23.64513106948669 27.275260745563322 27.2079620043759 21.871646180574082 20 23.24326647861771 27.9000721858987 28.00798046978642 20.848680865697172 21 22.60908489931195 27.255587275330612 27.13107974707185 23.004248078285592 22 22.369987178441995 28.55086749327424 27.419309414581 21.659835913702764 23 21.803550597571768 28.135009864417988 27.826107579222914 22.235331958787334 24 23.125780029466956 27.069579369573145 28.268860779006722 21.53577982195318 25 22.235924678651045 27.547075843096337 28.191552435611626 22.02544704264099 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 169.0 2 242.0 3 315.0 4 315.0 5 315.0 6 481.5 7 648.0 8 648.0 9 648.0 10 924.0 11 1200.0 12 1200.0 13 1200.0 14 2141.5 15 3083.0 16 3083.0 17 3083.0 18 5318.5 19 7554.0 20 7554.0 21 7554.0 22 11725.5 23 15897.0 24 15897.0 25 15897.0 26 23090.0 27 30283.0 28 30283.0 29 30283.0 30 40059.0 31 49835.0 32 49835.0 33 49835.0 34 57139.0 35 64443.0 36 64443.0 37 64443.0 38 70604.5 39 76766.0 40 76766.0 41 76766.0 42 80874.5 43 84983.0 44 84983.0 45 84983.0 46 87496.0 47 90009.0 48 90009.0 49 90009.0 50 86853.5 51 83698.0 52 83698.0 53 83698.0 54 74136.0 55 64574.0 56 64574.0 57 64574.0 58 54961.0 59 45348.0 60 45348.0 61 45348.0 62 36930.5 63 28513.0 64 28513.0 65 28513.0 66 22066.0 67 15619.0 68 15619.0 69 15619.0 70 11417.5 71 7216.0 72 7216.0 73 7216.0 74 5088.5 75 2961.0 76 2961.0 77 2961.0 78 2042.0 79 1123.0 80 1123.0 81 1123.0 82 742.0 83 361.0 84 361.0 85 361.0 86 248.5 87 136.0 88 136.0 89 136.0 90 79.5 91 23.0 92 23.0 93 23.0 94 16.5 95 10.0 96 10.0 97 10.0 98 8.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19191610820808153 2 0.11663164259440939 3 0.11841001579788195 4 0.07632184998236446 5 0.025638213683396218 6 0.024008038246879696 7 0.26749696935566575 8 0.2624582452791601 9 0.02148867620862689 10 0.03882781494248445 11 0.017931929801681748 12 0.019117511937330127 13 0.01733913873385756 14 0.01896931417037408 15 0.017042743199945466 16 0.02193326950949503 17 0.020006698539066413 18 0.016894545432989416 19 0.02519362038252808 20 0.018821116403418034 21 0.017190940966901512 22 0.019117511937330127 23 0.018821116403418034 24 0.0177837320347257 25 0.017487336500813605 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 674774.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.3248356130463 #Duplication Level Percentage of deduplicated Percentage of total 1 94.07004622370943 82.14651322597093 2 4.498776860229429 7.857098995586231 3 0.7267734076571324 1.9039610505477773 4 0.24855923930074894 0.8682157884816697 5 0.12597866529992513 0.550053311903347 6 0.07079733632155007 0.37094194536725467 7 0.04986216084336607 0.3047943499270751 8 0.03400853066410605 0.23758314797474442 9 0.025615061494485846 0.20131479308016434 >10 0.1260026985738487 2.045017492769205 >50 0.01102676008678801 0.6387754811003936 >100 0.01119596870610571 1.8459010325548304 >500 0.0010178153347952079 0.6820101391435935 >1k 3.3927177826506924E-4 0.34781924559277966 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1295 0.19191610820808153 No Hit GTCCTACAGTGTGCATTTCTCATTT 1053 0.15605224860471803 No Hit CTGTAGGACGTGGAATATGGCAAGA 999 0.14804956918909146 No Hit GTCCTAAAGTGTGTATTTCTCATTT 868 0.12863566171784924 No Hit GTATCAACGCAGAGTACTTTTTTTT 846 0.1253753108448162 No Hit CTGTAGGACCTGGAATATGGCGAGA 701 0.1038866346361893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.481977669560475E-4 0.0 2 0.0 0.0 0.0 1.481977669560475E-4 0.0 3 0.0 0.0 0.0 1.481977669560475E-4 0.0 4 0.0 0.0 0.0 1.481977669560475E-4 0.0 5 0.0 0.0 0.0 1.481977669560475E-4 0.0 6 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 7 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 8 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 9 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 10 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 11 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 12 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 13 0.0 0.0 0.0 1.481977669560475E-4 1.481977669560475E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGATC 50 0.0014883264 13.311447 8 CTTAGAC 60 3.983911E-4 12.704953 3 AGGACCG 60 4.026685E-4 12.688886 5 TCCAACG 75 1.4999578E-5 12.646548 18 GTCCTAA 295 0.0 12.6076145 1 TCTGGAC 125 1.3387762E-9 12.196755 3 GTATTAA 95 9.898704E-7 12.046142 1 TAGGACT 120 9.669748E-9 11.89937 4 GTCTAGA 75 1.9979585E-4 11.443834 1 TAAAGTG 360 0.0 11.367126 5 CGTGGAA 285 0.0 11.315332 9 TAGGACC 320 0.0 11.304402 4 TGTAGGA 675 0.0 11.152957 2 CTGTAGG 685 0.0 11.137552 1 GTAGGAC 655 0.0 11.056219 3 CTTACAC 95 1.3122542E-5 11.033249 3 TTAGCCA 70 0.0014642348 10.879425 4 CTAAAGT 365 0.0 10.693133 4 GTGTAGG 90 9.1332564E-5 10.596144 1 ACTACTC 90 9.413304E-5 10.56464 8 >>END_MODULE