FastQCFastQC Report
Thu 26 May 2016
SRR1926945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674774
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15520.23000293431578575No Hit
GTCCTACAGTGGACATTTCTAAATT12620.18702558189853197No Hit
CTGTAGGACGTGGAATATGGCAAGA11930.17679993597856467No Hit
GTCCTACAGTGTGCATTTCTCATTT10590.15694143520645432No Hit
GGTATCAACGCAGAGTACTTTTTTT9700.14375183394736607No Hit
CTGTAGGACCTGGAATATGGCGAGA9130.13530456123087137No Hit
GTCCTAAAGTGTGTATTTCTCATTT8440.1250789153109041No Hit
TATCAACGCAGAGTACTTTTTTTTT8160.12092937783613475No Hit
CTTTAGGACGTGAAATATGGCGAGG8120.12033658676831058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC601.4060679E-615.8945471
GCGCCAC602.6003121E-514.2287713
GCTTTAG759.2383016E-713.9872021
TTAGACT707.0509122E-613.6076774
GTCCTAT852.5677764E-713.4636151
GTTCTAA500.001455066513.3514191
TTGTACC500.001468192713.3355244
GTCCTAG801.9100062E-613.1130011
TAGGACC3350.013.0796184
GTGTTAG905.143247E-712.7156381
ATAGCCC603.9907085E-412.702393
GGACTGC603.995724E-412.7004996
GTAGGAC7550.012.49208453
CTTAGAC550.0030013512.1250083
TGTAGGA8150.012.0516552
ACTGTTC1750.011.9268318
GTCTAGA1052.5783993E-711.8073781
TAGGACG6250.011.735264
CTGTAGG8050.011.7283991
TTAGGAC3550.011.5394943
Produced by FastQC (version 0.11.5)