FastQCFastQC Report
Thu 26 May 2016
SRR1926944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809486
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT21500.26560064040638137No Hit
CTGTAGGACGTGGAATATGGCAAGA18180.22458696012037266No Hit
GTCCTAAAGTGTGTATTTCTCATTT17310.21383939932253307No Hit
GTCCTACAGTGTGCATTTCTCATTT15060.1860439834660513No Hit
CTTTAGGACGTGAAATATGGCGAGG13310.1644253266887877No Hit
CTGTAGGACCTGGAATATGGCGAGA9870.12192922422376669No Hit
GTATCAACGCAGAGTACTTTTTTTT9820.12131154831584487No Hit
GTACATGGGAAGCAGTGGTATCAAC8460.10451076362037144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG755.9424565E-815.17706918
GCCGGTT508.827846E-515.17519211
TGGCGAA400.0053293214.22850118
TGCCGGT602.6050735E-514.22674410
CCTACAC1500.013.9770743
CCAACGT551.9835652E-413.79733519
TAGGACC4450.013.688824
GTCTAGG1051.888111E-812.71353151
CCGGTTT604.1449766E-412.64599412
GGCGAGG4250.012.49876319
TCCTACA13200.012.3454512
GTCCTAC12600.012.3351531
CGGTTTC701.1051202E-412.19585813
GGACCTG7900.012.1640676
AGGACCT7800.012.0760565
AATGGCG550.003101127512.07266816
ATAGGAC1209.5278665E-911.9122783
CCTACAG12900.011.8199353
TTAGGAC5750.011.7672593
CTACACT1800.011.6331214
Produced by FastQC (version 0.11.5)