##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926944_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	809486
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.647394766555568	31.0	28.0	31.0	16.0	33.0
2	28.22969143382344	31.0	28.0	31.0	16.0	34.0
3	28.144979653755595	31.0	28.0	33.0	16.0	34.0
4	31.900796307780492	35.0	32.0	37.0	19.0	37.0
5	31.701273400651772	35.0	32.0	37.0	19.0	37.0
6	31.372593225824783	35.0	32.0	37.0	17.0	37.0
7	31.483888541617766	35.0	32.0	37.0	17.0	37.0
8	31.30467605369333	35.0	32.0	37.0	15.0	37.0
9	33.30667608828318	37.0	33.0	39.0	17.0	39.0
10	33.37089955848526	37.0	34.0	39.0	17.0	39.0
11	33.55441354143247	38.0	34.0	39.0	19.0	39.0
12	33.391137091932414	38.0	34.0	39.0	17.0	39.0
13	33.52094662538944	38.0	34.0	39.0	19.0	39.0
14	34.5556093619902	39.0	34.0	41.0	18.0	41.0
15	34.1190002050684	39.0	33.0	41.0	16.0	41.0
16	34.22246091964531	39.0	34.0	41.0	17.0	41.0
17	34.454600820767745	39.0	34.0	41.0	17.0	41.0
18	34.50683520159706	39.0	34.0	41.0	17.0	41.0
19	33.86338367803767	39.0	33.0	41.0	10.0	41.0
20	34.12135602098122	39.0	34.0	41.0	13.0	41.0
21	34.12176862848771	39.0	34.0	41.0	11.0	41.0
22	34.15362217505923	39.0	34.0	41.0	10.0	41.0
23	34.15274754597362	39.0	34.0	41.0	2.0	41.0
24	32.83394647961793	39.0	32.0	41.0	2.0	41.0
25	33.4315800396795	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.00543183853303475
1101	2	-0.059362131711878874
1101	3	-0.7957319610238898
1101	4	-0.039667666550201375
1101	5	0.009780740638589691
1101	6	-1.9289807927399636
1101	7	-0.09107262448700126
1101	8	0.30269555201977383
1101	9	0.348405932980846
1101	10	-9.08440463008652
1101	11	-3.6880285023913615
1101	12	-1.3690200317792502
1101	13	-0.5973089826357594
1101	14	-0.43974329367001985
1101	15	-1.6006102860392986
1101	16	0.04446216972248607
1101	17	0.041683533597002054
1101	18	0.1528458267344206
1101	19	-0.462629322571253
1101	20	0.31941534596467847
1101	21	0.4116481798870595
1101	22	0.45720506349804424
1101	23	0.33523264262868224
1101	24	0.04651505652581989
1101	25	0.17060875553084287
1102	1	-0.42099335539050387
1102	2	-0.15277405347608664
1102	3	-0.4749551446109983
1102	4	-0.26526516112415877
1102	5	-0.18864245302395943
1102	6	-0.8549493993486124
1102	7	-0.03140083194030652
1102	8	0.6392530261070029
1102	9	0.5653070635291542
1102	10	0.3267824666335031
1102	11	0.021097747068132833
1102	12	0.27855997351035455
1102	13	-0.08569284010187062
1102	14	-0.22917709593111368
1102	15	0.45946872220157076
1102	16	0.24043997017000152
1102	17	-0.02230600473794908
1102	18	-0.16362164009009206
1102	19	0.3684412348944406
1102	20	0.09452733028057736
1102	21	0.09488474701677774
1102	22	-0.06874339223089265
1102	23	-0.17345742424705435
1102	24	-3.845698727515522
1102	25	-2.2013722858498284
1103	1	-0.25148301298078124
1103	2	-0.13916920754098427
1103	3	0.19484040011150583
1103	4	-0.0015879711877637703
1103	5	-0.20355783836706465
1103	6	0.5915744713893503
1103	7	0.5277709961926043
1103	8	0.6421342650814985
1103	9	0.35453559822923353
1103	10	0.4004509196405124
1103	11	0.22368877529898867
1103	12	-0.06876357668803479
1103	13	0.2019469090313919
1103	14	0.11592883238154172
1103	15	0.26646049168019204
1103	16	0.35814366027056366
1103	17	-0.06107821573757377
1103	18	0.06647680340089579
1103	19	-1.4585007841709867
1103	20	-0.3454201924938687
1103	21	-0.17071373264621315
1103	22	0.07787981238576691
1103	23	6.898903992720307E-4
1103	24	1.4939466199304547
1103	25	0.6437586846171257
1104	1	-0.09726623692706227
1104	2	0.13516783104166308
1104	3	-1.0891572043933273
1104	4	-0.08329495118375263
1104	5	-0.07678763789338561
1104	6	-1.2482877605401086
1104	7	0.08995089679581625
1104	8	0.554832828326365
1104	9	0.5750458061820609
1104	10	0.5687317453817826
1104	11	0.35025167330617535
1104	12	0.6154537955296391
1104	13	0.32786215619555037
1104	14	0.4142357267467105
1104	15	0.6711962013591872
1104	16	0.2478533284240214
1104	17	0.40022125909617756
1104	18	0.31461921071095134
1104	19	-0.8627514331767472
1104	20	-0.09929796804356528
1104	21	0.07376091141681229
1104	22	0.376860101037515
1104	23	0.5026883945697094
1104	24	1.653310729776372
1104	25	1.0997048781102663
1105	1	0.06879923061017479
1105	2	0.24341349134250834
1105	3	0.8529938339672434
1105	4	0.48601765425247834
1105	5	0.41790095995210663
1105	6	0.11261724513837379
1105	7	0.3783462517042899
1105	8	0.6999641092780706
1105	9	-0.16616712300056946
1105	10	0.21953488292563605
1105	11	0.10111603509641753
1105	12	0.057148965042614464
1105	13	0.0034034673220659784
1105	14	-0.10238758378876156
1105	15	0.459783047687651
1105	16	0.1522567034437401
1105	17	-0.10055332038469089
1105	18	0.06492632584301106
1105	19	-0.7312836282515107
1105	20	0.06042641683166039
1105	21	0.15151504173434205
1105	22	0.08363439162475572
1105	23	-0.13625883993638155
1105	24	-5.107476068049767
1105	25	-2.526401167055205
1106	1	-0.2544562725823276
1106	2	-0.8963466940950333
1106	3	-0.6234282375458164
1106	4	-0.8696715579290277
1106	5	-0.31149067895273674
1106	6	-0.0824004439309185
1106	7	-2.849946600959125
1106	8	-0.506028211787136
1106	9	-0.6421962510895369
1106	10	-0.2121677131470463
1106	11	-0.7310792617544806
1106	12	-0.35776224562004444
1106	13	-0.5579387056947667
1106	14	-0.6297262586829859
1106	15	-0.15429226514558536
1106	16	-0.022563429019207604
1106	17	-0.4994378638161834
1106	18	-0.3969025942637856
1106	19	0.1323094358339887
1106	20	-0.06033356485743724
1106	21	-0.438058197892353
1106	22	-0.7177525531276672
1106	23	-0.8364139004390836
1106	24	-4.454190425964335
1106	25	-2.504606698957712
1107	1	0.011949224388906998
1107	2	-0.3695220543733839
1107	3	0.09777888103715426
1107	4	-0.4101112842820491
1107	5	-0.1583922982135526
1107	6	-0.2387039110141309
1107	7	-2.4830549861650546
1107	8	-2.2945996105056246
1107	9	-0.9186342952293529
1107	10	-0.27202015379287303
1107	11	-0.7336946592295917
1107	12	-0.42877045032762595
1107	13	-0.648494149509105
1107	14	-0.4749750512248241
1107	15	-1.7200213504552195
1107	16	-1.0474583559970512
1107	17	-0.9828619252117434
1107	18	-0.941485162460026
1107	19	-0.0028357595404457925
1107	20	-0.06689458818639338
1107	21	-0.4806862380878414
1107	22	-0.5513159717819462
1107	23	-0.7161447985685356
1107	24	-4.1723668180349485
1107	25	-2.2663684777153286
1108	1	0.5339678104705214
1108	2	0.05408365563515716
1108	3	0.5266362115884498
1108	4	0.45339554924768777
1108	5	0.4329123205797174
1108	6	-3.280866322053601
1108	7	-1.3334410356020214
1108	8	0.20082509475051324
1108	9	0.14916808154548988
1108	10	0.5258853030466
1108	11	0.45032955240728256
1108	12	0.7289250688788158
1108	13	0.5563629317523819
1108	14	0.36447913127948084
1108	15	-0.406319377695219
1108	16	0.4907112844469097
1108	17	0.3731201081155078
1108	18	0.42040542093536004
1108	19	0.4281748753409218
1108	20	-0.9388340311126981
1108	21	0.046804004950644185
1108	22	0.11062224377361218
1108	23	0.2736572131747934
1108	24	-0.8952220512449287
1108	25	-0.1954226327468831
1109	1	0.4421695177989733
1109	2	0.3632886127964525
1109	3	-0.9723558737468743
1109	4	0.2697038013351971
1109	5	0.6402510340191014
1109	6	0.8002501276983871
1109	7	0.8003382556545269
1109	8	1.3065463554990586
1109	9	0.44920695514409203
1109	10	0.7188171498535283
1109	11	0.5228614676317349
1109	12	0.2196280590360189
1109	13	0.3622113869155683
1109	14	0.4051060846778114
1109	15	-1.054800663944846
1109	16	0.0633368072968139
1109	17	0.06089233992655352
1109	18	0.39317213269540474
1109	19	-1.661652370386868
1109	20	-0.3131936274492517
1109	21	0.10702387402436386
1109	22	0.18155318871304615
1109	23	0.3491733768170917
1109	24	-1.0327910518824552
1109	25	-0.2970152063346063
1110	1	0.6792691280591008
1110	2	0.6088241274645689
1110	3	0.959337530887705
1110	4	0.7232250557537583
1110	5	1.054374119308683
1110	6	1.057218011924494
1110	7	1.294119940901819
1110	8	1.4203606701309255
1110	9	1.0959727184602102
1110	10	1.2297461809420795
1110	11	0.7967247263404573
1110	12	0.8543330350171701
1110	13	0.7658556057741492
1110	14	0.5379094926357553
1110	15	1.0986275363028923
1110	16	0.7705158761508599
1110	17	0.549581424188915
1110	18	0.694468244360408
1110	19	-1.276733792786338
1110	20	0.27707979470851996
1110	21	0.4616660082278159
1110	22	0.6560309674979052
1110	23	0.7160672406800472
1110	24	-0.1674990491029149
1110	25	0.12327483263938177
1111	1	0.5362293667781834
1111	2	0.25593085511334834
1111	3	1.0015504685047638
1111	4	0.8358266688657281
1111	5	0.8020744702081544
1111	6	0.6580301842534944
1111	7	0.5261677823939088
1111	8	0.9008927089374268
1111	9	0.4467427682014886
1111	10	0.30327878870875224
1111	11	-0.030682549024056982
1111	12	0.2620723584673641
1111	13	0.11887463541624044
1111	14	0.2266479882855279
1111	15	-0.6707983023028845
1111	16	-0.39897076549458177
1111	17	-0.1307337748616817
1111	18	0.22232287381594062
1111	19	0.8779977337118225
1111	20	0.3612956674763268
1111	21	0.4570813613616167
1111	22	0.6991119967053407
1111	23	0.18056705631742176
1111	24	0.8417373965809247
1111	25	0.5555661304790434
1112	1	0.29707268801077547
1112	2	0.3295824734401691
1112	3	-1.1739771112935529
1112	4	-0.25777590295978214
1112	5	0.05802780394063589
1112	6	0.8702100654128735
1112	7	0.7103121017851173
1112	8	1.0978735509878987
1112	9	-0.13887111340020653
1112	10	0.4561403844673393
1112	11	0.2673366516914797
1112	12	0.32179142245386316
1112	13	0.10997258545820898
1112	14	0.2388472974312208
1112	15	0.6084790010437402
1112	16	0.45675994062107605
1112	17	-0.2923306331485733
1112	18	0.18733966863807439
1112	19	0.844020751599011
1112	20	0.5773513708121953
1112	21	0.4721863152537509
1112	22	0.39378836188794253
1112	23	0.43690161846070197
1112	24	1.5577784372856058
1112	25	0.6580249315401261
1113	1	-0.5169289721717298
1113	2	-0.06072761438407426
1113	3	-4.666437460404527
1113	4	-1.149949117303521
1113	5	-0.026217300441121694
1113	6	0.8333445608739574
1113	7	0.8185577733466651
1113	8	1.152820865302985
1113	9	0.6539398827979142
1113	10	0.6301806837728421
1113	11	0.46516482575659523
1113	12	0.2673179395859435
1113	13	-0.1423727915834192
1113	14	-0.4152049362823007
1113	15	0.25269539194877666
1113	16	0.6391673234113071
1113	17	0.4213971855429577
1113	18	0.2325681209757775
1113	19	1.075503365470695
1113	20	0.40798735068913317
1113	21	0.5800461863975883
1113	22	0.7701310566418229
1113	23	0.5693581863731012
1113	24	1.7667581931028948
1113	25	1.071170504731434
1114	1	0.686364754175731
1114	2	0.22389970385864544
1114	3	0.6076922436595957
1114	4	0.3376034765427036
1114	5	0.9857905504391624
1114	6	1.1701749442507712
1114	7	1.1247891157531704
1114	8	1.0021051188817154
1114	9	0.29041650644927586
1114	10	0.37713905530281977
1114	11	0.12931521477476338
1114	12	-0.3717108077990545
1114	13	0.009016391125840073
1114	14	0.11176072199285869
1114	15	-2.364305054658189
1114	16	-0.14463083374733543
1114	17	0.040194637532707134
1114	18	0.2592493611327953
1114	19	0.8492540412575451
1114	20	0.7949376127769057
1114	21	0.4886069366972876
1114	22	0.6397517933082568
1114	23	0.008603185550398962
1114	24	1.101588835041639
1114	25	0.7893597485553272
1115	1	-3.765503348624449
1115	2	-0.8849472745993516
1115	3	0.005342206998282251
1115	4	0.6979586736320726
1115	5	1.0984498415299981
1115	6	1.5248302350817333
1115	7	1.4853229787419515
1115	8	1.6891799172444877
1115	9	1.299617800268834
1115	10	0.2174929530038341
1115	11	0.5696384840358775
1115	12	0.3482477974003544
1115	13	0.8069498995672291
1115	14	0.7839064762413983
1115	15	1.2531990987438917
1115	16	1.1559884640260734
1115	17	1.0291869618600415
1115	18	1.0105789138647552
1115	19	-1.6533203719293894
1115	20	0.1871753915653187
1115	21	0.43331796200751427
1115	22	0.7211503528434449
1115	23	0.5442574739960548
1115	24	-1.074032783068123
1115	25	-0.09056864586472813
1116	1	0.8533086683232298
1116	2	-0.3310648022855922
1116	3	-0.14441143917546384
1116	4	-0.2523683507884904
1116	5	0.8457566320286958
1116	6	1.1231409517017035
1116	7	0.6779325924829145
1116	8	-1.7994953481914777
1116	9	-0.4203386878865345
1116	10	-0.31894364630783656
1116	11	0.2604895702322949
1116	12	0.44826676527380016
1116	13	-0.04480360034821729
1116	14	0.15344978469558157
1116	15	-0.4929216283426925
1116	16	-0.11356444262914067
1116	17	0.10166480601268546
1116	18	0.15322189278070653
1116	19	0.7904227436466584
1116	20	0.42378818642433913
1116	21	0.37800604332713306
1116	22	0.3413369692947654
1116	23	0.47590342835432153
1116	24	1.5297763315663886
1116	25	0.9300400629029966
1201	1	0.09723554420953562
1201	2	0.2707936147972525
1201	3	0.8471699365772558
1201	4	0.6979651058570084
1201	5	0.43119141016956064
1201	6	1.396490623896863
1201	7	1.0711185677793829
1201	8	1.4004399129220317
1201	9	1.0149532536634993
1201	10	0.907688698472974
1201	11	0.9411187705529045
1201	12	1.040396366358948
1201	13	0.7199424793525466
1201	14	0.7234474212124269
1201	15	1.3272301483800817
1201	16	0.8811819453520329
1201	17	0.8096898589341563
1201	18	0.6553670106465859
1201	19	0.011084724451066563
1201	20	0.820593887556754
1201	21	0.8491407207630601
1201	22	1.0678537089211275
1201	23	1.0379268782176538
1201	24	-0.06073222835304648
1201	25	0.5064781910243426
1202	1	-0.5583767006884237
1202	2	-0.13651931037281528
1202	3	-0.265925301517985
1202	4	-0.14132060842870686
1202	5	-0.28224396398010043
1202	6	0.7094157939648902
1202	7	0.5362546222011453
1202	8	1.0364943346907367
1202	9	0.5608716210104419
1202	10	0.5837091066362419
1202	11	0.2361867977637857
1202	12	0.6560426248623514
1202	13	0.13405812561104824
1202	14	0.3433453803960944
1202	15	0.7609036177678377
1202	16	0.513835006576528
1202	17	0.3088395187981021
1202	18	0.08733979976223338
1202	19	-0.013575139494513166
1202	20	0.43879116646832017
1202	21	0.17141963232768376
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2211	1	0.7280518707401491
2211	2	0.5014032432640256
2211	3	0.25535151690169045
2211	4	0.5214685072175484
2211	5	0.7663143824353895
2211	6	-2.030398439420768
2211	7	0.19750265987775606
2211	8	0.41230804479016214
2211	9	0.642268795264755
2211	10	0.8341565163693616
2211	11	0.6215190845246497
2211	12	0.7823429911627215
2211	13	0.713056032466099
2211	14	0.6692689806054091
2211	15	1.2551239212738565
2211	16	0.44119502750797324
2211	17	0.5287505601377092
2211	18	0.5394617254555882
2211	19	0.6107759855708963
2211	20	0.5117686651601048
2211	21	0.1229816417468399
2211	22	-2.9030018640773037
2211	23	-1.1984284331376571
2211	24	1.0176011049802867
2211	25	0.5893196352943519
2212	1	-0.6326536507322444
2212	2	-0.20018005074258127
2212	3	-3.190620639775986
2212	4	-1.93310204492969
2212	5	-0.6231051550823103
2212	6	0.22845715283973078
2212	7	0.4261694838513286
2212	8	0.01820941336024262
2212	9	0.2543735616640035
2212	10	0.491402858983804
2212	11	0.27962196324101996
2212	12	0.3238413394402073
2212	13	0.14321884511357297
2212	14	0.047343544078749744
2212	15	-0.9363909442287408
2212	16	-0.07254494417678359
2212	17	0.0033848215817897653
2212	18	0.05449475990580055
2212	19	0.7457264338955838
2212	20	-0.4903384608427288
2212	21	-0.5850126952045542
2212	22	0.032389830118070506
2212	23	0.0914252714399737
2212	24	1.3009753381091258
2212	25	0.8468750859786383
2213	1	-0.5408974867806364
2213	2	-0.7113492423456051
2213	3	-0.15727072857769642
2213	4	-7.111275256954784
2213	5	-1.9558078339725427
2213	6	-3.5340649316586905
2213	7	-1.590257093596282
2213	8	-3.4536795126259676
2213	9	-1.2715168899494955
2213	10	-0.6748887769616445
2213	11	-0.5189152430525397
2213	12	-0.5155371181459643
2213	13	-0.46467581240860056
2213	14	-0.6600559801481225
2213	15	-2.8159104411890787
2213	16	-1.3893207001506127
2213	17	-0.7338664524792975
2213	18	-0.6073880913942347
2213	19	0.0857256919491789
2213	20	-1.8395535047334945
2213	21	-0.7198313124946907
2213	22	-0.8970933810259609
2213	23	-0.6815553591217309
2213	24	-1.0474102328881898
2213	25	-0.682675248129101
2214	1	0.6940111730657321
2214	2	0.5534180017613402
2214	3	0.47017262602512844
2214	4	0.5691138560547628
2214	5	0.7428707330615083
2214	6	0.6588502188976584
2214	7	1.0205035543321408
2214	8	-2.4504737083404287
2214	9	-0.8675366609069783
2214	10	-0.3984485820206203
2214	11	0.22846452280729324
2214	12	-1.0118698889803923
2214	13	-0.30023332352674714
2214	14	-0.09223146662177584
2214	15	-1.5406408372234637
2214	16	-0.22190202079256238
2214	17	0.008793046720896314
2214	18	0.02962049110853826
2214	19	0.917122139417053
2214	20	-0.9273346443211139
2214	21	-0.10613732068608073
2214	22	0.294138118033608
2214	23	-0.09056841524856907
2214	24	-1.4941140112987803
2214	25	-0.5958592555822122
2215	1	0.5914169036589492
2215	2	0.030311577066285622
2215	3	0.27223239023464885
2215	4	0.5291641911970686
2215	5	0.9139357880962962
2215	6	1.1507821356734844
2215	7	0.5104439687517619
2215	8	-0.6130411195787531
2215	9	-0.08919257438242312
2215	10	-0.04874427705813389
2215	11	-0.2943308957803481
2215	12	-0.5306365822996639
2215	13	-0.15518379646868397
2215	14	-0.26240284427782257
2215	15	-0.2635795844377
2215	16	-0.05614108188373024
2215	17	-0.5446133473520192
2215	18	-1.3351896728900314
2215	19	-0.029247648439564955
2215	20	-3.555549437366487
2215	21	-2.0295511363748204
2215	22	-1.1443512535320295
2215	23	-1.018849343609773
2215	24	-0.9877317406671153
2215	25	-0.819220120487536
2216	1	-0.1156573280729063
2216	2	-1.0611897720292873
2216	3	-0.42088906743978427
2216	4	-0.6952377242364562
2216	5	-0.4411314061973428
2216	6	-1.7851667571564462
2216	7	-10.198507383491336
2216	8	-10.983727107001666
2216	9	-5.824188634545788
2216	10	-3.3916663551211634
2216	11	-2.241245124560038
2216	12	-1.9251005148861289
2216	13	-1.7727097744273053
2216	14	-1.9708565615082634
2216	15	-3.1371792058660652
2216	16	-1.949756809972051
2216	17	-2.326533621555967
2216	18	-2.1398877381372046
2216	19	-1.6802443294256761
2216	20	-2.451860643216982
2216	21	-4.883468940312053
2216	22	-3.700472761132769
2216	23	-3.6712542719872374
2216	24	-3.4540309424974573
2216	25	-3.0707488422221942
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44780.0
3	4.0
4	372.0
5	2186.0
6	2678.0
7	2183.0
8	1930.0
9	1683.0
10	1534.0
11	1471.0
12	1510.0
13	1487.0
14	1526.0
15	1567.0
16	1604.0
17	1725.0
18	2057.0
19	2523.0
20	3252.0
21	4107.0
22	5250.0
23	6292.0
24	7214.0
25	8432.0
26	9761.0
27	10545.0
28	11723.0
29	13906.0
30	16678.0
31	20440.0
32	25971.0
33	34133.0
34	46020.0
35	64229.0
36	93058.0
37	153269.0
38	202386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84039418624805	18.625424022581523	12.713001708470548	25.82118008269988
2	14.054790826884092	19.844698717243066	42.822881583390334	23.27762887248251
3	18.99790715278774	24.273069558720938	29.56146687314773	27.167556415343586
4	11.529052252394804	16.664791782816753	38.93111841560365	32.875037549184796
5	12.738826744888618	38.17236918355016	36.34898884373444	12.739815227826783
6	31.81103782653714	37.375297469746435	18.43410442763485	12.379560276081579
7	27.850116457703557	31.345458149561424	22.52279597601467	18.281629416720353
8	24.250367940376314	38.11057041908037	21.186180308128385	16.45288133241493
9	26.078866243690886	15.648456467884525	20.493238359414097	37.779438929010496
10	14.258385740996365	29.52047760692337	35.20868115585621	21.012455496224053
11	34.017663426230236	22.65319587425064	25.079075215356404	18.250065484162718
12	23.451506206824973	24.8370608055096	32.27894942985182	19.432483557813608
13	29.04388007882723	22.532849826716006	25.7430207631909	22.68024933126587
14	19.763910476750592	23.121897216792654	27.917079547392117	29.197112759064638
15	22.532766306550293	32.571092506746005	22.815703116505716	22.08043807019798
16	20.94091016922347	28.62033943539459	29.439892060730667	20.998858334651274
17	20.89012830143919	29.49524553218409	28.612555353471453	21.002070812905266
18	20.67099433383661	28.046396809354484	30.931370974318167	20.35123788249074
19	23.60999562592517	27.1249477952933	27.475985834916976	21.789070743864556
20	23.24642365564295	28.065994024805214	28.530072428849962	20.157509890701874
21	22.255581872613085	27.21826230816657	27.36813362640404	23.158022192816308
22	22.253042565021314	29.43164267622166	27.50800024711188	20.807314511645146
23	21.197901029098695	28.861838341679547	28.330794255630142	21.609466373591616
24	23.06854981695255	27.247319772707336	28.95510364451605	20.72902676582406
25	21.807670733244212	28.056172639684096	28.682221305176174	21.453935321895518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	192.0
1	192.0
2	272.0
3	352.0
4	352.0
5	352.0
6	534.0
7	716.0
8	716.0
9	716.0
10	1085.0
11	1454.0
12	1454.0
13	1454.0
14	2586.0
15	3718.0
16	3718.0
17	3718.0
18	6678.5
19	9639.0
20	9639.0
21	9639.0
22	15432.0
23	21225.0
24	21225.0
25	21225.0
26	30669.5
27	40114.0
28	40114.0
29	40114.0
30	53308.0
31	66502.0
32	66502.0
33	66502.0
34	75788.5
35	85075.0
36	85075.0
37	85075.0
38	91087.0
39	97099.0
40	97099.0
41	97099.0
42	100893.0
43	104687.0
44	104687.0
45	104687.0
46	106422.5
47	108158.0
48	108158.0
49	108158.0
50	101551.5
51	94945.0
52	94945.0
53	94945.0
54	82254.0
55	69563.0
56	69563.0
57	69563.0
58	59122.0
59	48681.0
60	48681.0
61	48681.0
62	39201.0
63	29721.0
64	29721.0
65	29721.0
66	22757.5
67	15794.0
68	15794.0
69	15794.0
70	11491.5
71	7189.0
72	7189.0
73	7189.0
74	5085.0
75	2981.0
76	2981.0
77	2981.0
78	2055.5
79	1130.0
80	1130.0
81	1130.0
82	760.0
83	390.0
84	390.0
85	390.0
86	251.5
87	113.0
88	113.0
89	113.0
90	73.5
91	34.0
92	34.0
93	34.0
94	21.0
95	8.0
96	8.0
97	8.0
98	7.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2156924270462985
2	0.12267043531327287
3	0.12575881485288196
4	0.06757374432664678
5	0.020383304961419962
6	0.019765629053498147
7	0.2873428323652293
8	0.2828955658281922
9	0.01741846060339524
10	0.03520752675154357
11	0.016059573605967246
12	0.01667724951388906
13	0.014947756971707974
14	0.01630664396913597
15	0.014577151426954881
16	0.017541995784979603
17	0.017541995784979603
18	0.01482422179012361
19	0.021248051232510504
20	0.017294925421810877
21	0.015441897698045427
22	0.016800784695473425
23	0.016924319877057788
24	0.016430179150720335
25	0.015318362516461063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	809486.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.31316368798068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.24105582761699	78.61448402434016
2	5.065271095808358	8.541380620497746
3	0.8464222227335607	2.1409360624343745
4	0.2919903258377406	0.9847451255065695
5	0.1470551480865246	0.6199342385889693
6	0.08607161648944892	0.43541821739784026
7	0.05910975117228341	0.3488611088301177
8	0.04058210471119517	0.2737284509854216
9	0.02715811633478236	0.20608080371925544
>10	0.16232510895039662	2.4979532243209754
>50	0.016993191952488898	1.0049968887670877
>100	0.013768404548944553	2.4045809368611453
>500	0.001464723838119269	0.8727427035955703
>1k	7.323619190596346E-4	1.054157594154774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	2150	0.26560064040638137	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1818	0.22458696012037266	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1731	0.21383939932253307	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1506	0.1860439834660513	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1331	0.1644253266887877	No Hit
CTGTAGGACCTGGAATATGGCGAGA	987	0.12192922422376669	No Hit
GTATCAACGCAGAGTACTTTTTTTT	982	0.12131154831584487	No Hit
GTACATGGGAAGCAGTGGTATCAAC	846	0.10451076362037144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.235351815843634E-4
11	0.0	0.0	0.0	0.0	1.235351815843634E-4
12	0.0	0.0	0.0	0.0	2.470703631687268E-4
13	0.0	0.0	0.0	0.0	2.470703631687268E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	75	5.9424565E-8	15.177069	18
GCCGGTT	50	8.827846E-5	15.175192	11
TGGCGAA	40	0.00532932	14.228501	18
TGCCGGT	60	2.6050735E-5	14.226744	10
CCTACAC	150	0.0	13.977074	3
CCAACGT	55	1.9835652E-4	13.797335	19
TAGGACC	445	0.0	13.68882	4
GTCTAGG	105	1.888111E-8	12.7135315	1
CCGGTTT	60	4.1449766E-4	12.645994	12
GGCGAGG	425	0.0	12.498763	19
TCCTACA	1320	0.0	12.345451	2
GTCCTAC	1260	0.0	12.335153	1
CGGTTTC	70	1.1051202E-4	12.195858	13
GGACCTG	790	0.0	12.164067	6
AGGACCT	780	0.0	12.076056	5
AATGGCG	55	0.0031011275	12.072668	16
ATAGGAC	120	9.5278665E-9	11.912278	3
CCTACAG	1290	0.0	11.819935	3
TTAGGAC	575	0.0	11.767259	3
CTACACT	180	0.0	11.633121	4
>>END_MODULE