FastQCFastQC Report
Thu 26 May 2016
SRR1926944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809486
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA23370.2887017193626573No Hit
GTCCTACAGTGGACATTTCTAAATT22230.27461870866203986No Hit
CTTTAGGACGTGAAATATGGCGAGG17490.2160630325910516No Hit
GTCCTAAAGTGTGTATTTCTCATTT17000.21000980869341782No Hit
GTATCAACGCAGAGTACTTTTTTTT16540.20432719034053706No Hit
GTCCTACAGTGTGCATTTCTCATTT15540.19197367218210076No Hit
CTGTAGGACCTGGAATATGGCGAGA12750.15750735652006334No Hit
GGTATCAACGCAGAGTACTTTTTTT10320.12748830739506303No Hit
GTACATGGGAAGCAGTGGTATCAAC9100.1124170152417707No Hit
CTGAAGGACCTGGAATATGGCGAGA9010.11130519860751142No Hit
TATCAACGCAGAGTACTTTTTTTTT8550.10562258025463073No Hit
CCCATGTACTCTGCGTTGATACCAC8200.101298848899178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4600.014.6886684
AGGACCT7850.013.6990575
GGACCTG7800.013.5428576
TTAAGGC604.0284664E-412.6888033
GGTCTAC951.0024487E-612.03438951
TGTAGGA12700.012.0013142
AATACTG1052.6526504E-711.7824595
GTAGGAC12450.011.771543
AAGGACC3900.011.7127424
TAAACAG1156.885057E-811.5854285
CTGTAGG12500.011.5851051
GTCCTAC13200.011.5481521
TATACTG1251.7793354E-811.4199225
TCTAGGA1354.5129127E-911.2901252
GTCCTAA5050.011.1308171
CTCTAAG1201.2223063E-711.1150961
GACCTGG8450.011.0063257
GGCGAGG4750.010.99130719
CCAATAC700.001468510510.8761173
CTTAGAC1053.387273E-610.8761163